{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T07:34:58Z","timestamp":1789198498922,"version":"build-2803163510"},"reference-count":116,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R01AI127375"],"award-info":[{"award-number":["1R01AI127375"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["FDN-154288"],"award-info":[{"award-number":["FDN-154288"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002784","name":"Canada Excellence Research Chairs, Government of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002784","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007631","name":"Canadian Institute for Advanced Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007631","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosbiology.org"],"crossmark-restriction":false},"short-container-title":["PLoS Biol"],"abstract":"<jats:p>\n                    A small percentage of species in the fungal kingdom can cause devastating infections in humans, with\n                    <jats:italic>\n                      <jats:italic>Candida albicans<\/jats:italic>\n                    <\/jats:italic>\n                    reigning as a leading cause of systemic disease. One of the key virulence phenotypes for pathogenic fungi is the ability to survive at host body temperature; however, a comprehensive understanding of the mechanisms that orchestrate thermal adaptation in fungi remains incomplete. In this study, we expand the largest functional genomics resource in\n                    <jats:italic>\n                      <jats:italic>C. albicans,<\/jats:italic>\n                    <\/jats:italic>\n                    reaching 71.3% coverage of the entire genome, and perform screens under six different temperatures to identify genes important for temperature-dependent fitness. We describe the function of genes involved in translation (\n                    <jats:italic>GAR1<\/jats:italic>\n                    ), splicing (\n                    <jats:italic>\n                      <jats:italic>C1_11680C<\/jats:italic>\n                    <\/jats:italic>\n                    or\n                    <jats:italic>YSF3<\/jats:italic>\n                    ), and cell cycle progression (\n                    <jats:italic>\n                      <jats:italic>C6_00110C<\/jats:italic>\n                    <\/jats:italic>\n                    or\n                    <jats:italic>RHT1<\/jats:italic>\n                    ) in enabling fungal survival at both low and high temperatures. Through experimental evolution, we also show that\n                    <jats:italic>\n                      <jats:italic>C. albicans<\/jats:italic>\n                    <\/jats:italic>\n                    can rapidly overcome deleterious mutations and adapt to extreme temperature environments. Overall, our study highlights the transformative potential of genome-wide functional genomics to uncover critical vulnerabilities in pathogenic fungi.\n                  <\/jats:p>","DOI":"10.1371\/journal.pbio.3003409","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T17:24:41Z","timestamp":1760721881000},"page":"e3003409","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Expansion of the functional genomics GRACE library reveals genes relevant for temperature-dependent fitness in Candida albicans"],"prefix":"10.1371","volume":"23","author":[{"given":"Ci","family":"Fu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily H.","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia","family":"Kupczok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda S.","family":"Archambault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy R. 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