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Here, we identify the non-vesicular cholesterol transport protein GRAMD1C as a key host factor required for the replication of multiple coronaviruses across \u03b1, \u03b2, and \u03b4 genera, including TGEV, PEDV, HCoV-229E, SARS-CoV-2, MHV, and PDCoV. Mechanistically, GRAMD1C is recruited to the replication factory through its interaction with the viral nonstructural proteins 3 and 4 (nsp3 and nsp4). Transmission electron microscopy (TEM) analysis revealed that GRAMD1C facilitates the formation of replication double-membrane vesicles (DMVs). Further domain rescue and inhibitor experiments demonstrated that GRAMD1C\u2019s cholesterol transport activity is essential for viral replication. Moreover, GRAMD1C-deficient and inhibitor-treated mice exhibited reduced viral replication, underscoring its critical role in vivo. 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