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Here we study the neutralising epitopes on WIV-1 Spike of three mAbs that confer broad sarbecovirus and SARS-CoV-2 variant neutralisation, including XEC, JN.1, and XFG. mAb V1WT_06 binds a highly conserved RBD site V epitope that is mediated by the heavy chain alone. V1WT_06 contact residues are highly conserved in circulating viruses suggesting that the epitope is evolutionarily and functionally constrained. mAbs V1WT_41 and VA14_26 bind overlapping RBD class 4 epitopes with differing angles of approach that impact on the degree of ACE2 competition. We show that neutralisation by these mAbs is maintained when virus entry is via Japanese horseshoe bat and Halcyon horseshoe bat ACE2s. These mAbs are ideal candidates for therapeutic antibody development and inform the rational design of pan-betacoronavirus vaccines.<\/jats:p>","DOI":"10.1371\/journal.pbio.3003882","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:24:43Z","timestamp":1790875483000},"page":"e3003882","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting of conserved spike epitopes enables broad antibody neutralisation of diverse bat sarbecoviruses"],"prefix":"10.1371","volume":"24","author":[{"given":"Ayush","family":"Upadhyay","sequence":"first","affiliation":[{"name":"King\u2019s College London","place":["London, United Kingdom"],"department":["Department of Infectious Diseases, School of Immunology & Microbial Sciences"]},{"name":"The Francis Crick Institute","place":["London, United Kingdom"],"department":["Chromatin Structure and Mobile DNA Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Seow","sequence":"additional","affiliation":[{"name":"King\u2019s College London","place":["London, United Kingdom"],"department":["Department of Infectious Diseases, School of Immunology & Microbial Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilmaz","family":"Alguel","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute","place":["London, United Kingdom"],"department":["Chromatin Structure and Mobile DNA Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Newman","sequence":"additional","affiliation":[{"name":"The Pirbright Institute","place":["Woking, Surrey, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazia","family":"Thakur","sequence":"additional","affiliation":[{"name":"The Pirbright Institute","place":["Woking, Surrey, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail L.","family":"Hay","sequence":"additional","affiliation":[{"name":"The Pirbright Institute","place":["Woking, Surrey, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry C. H.","family":"Tam","sequence":"additional","affiliation":[{"name":"King\u2019s College London","place":["London, United Kingdom"],"department":["Department of Infectious Diseases, School of Immunology & Microbial Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Nans","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute","place":["London, United Kingdom"],"department":["Structural Biology Science Technology Platform"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"Orton","sequence":"additional","affiliation":[{"name":"The University of Glasgow","place":["Glasgow, United Kingdom"],"department":["MRC-University of Glasgow Centre for Virus Research"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalan","family":"Bailey","sequence":"additional","affiliation":[{"name":"The Pirbright Institute","place":["Woking, Surrey, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Cherepanov","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute","place":["London, United Kingdom"],"department":["Chromatin Structure and Mobile DNA Laboratory"]},{"name":"South Kensington Campus, Imperial College London","place":["London, United Kingdom"],"department":["Department of Infectious Disease"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5507-1725","authenticated-orcid":true,"given":"Katie J.","family":"Doores","sequence":"additional","affiliation":[{"name":"King\u2019s College London","place":["London, United Kingdom"],"department":["Department of Infectious Diseases, School of Immunology & Microbial 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