{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T18:03:56Z","timestamp":1784311436024,"version":"3.55.0"},"reference-count":72,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["214244\/Z\/18\/Z"],"award-info":[{"award-number":["214244\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/Z533890\/1"],"award-info":[{"award-number":["EP\/Z533890\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["493410640"],"award-info":[{"award-number":["493410640"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/Y029577\/1"],"award-info":[{"award-number":["EP\/Y029577\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosbiology.org"],"crossmark-restriction":false},"short-container-title":["PLoS Biol"],"abstract":"<jats:p>The molecular mechanisms underpinning neuronal morphogenesis remain to be fully understood. A powerful step in uncovering the molecular basis of biological processes is the execution of discovery screens in model systems. Here, we employed zebrafish to identify molecules that regulate axon diameter in the central nervous system. Axon diameter can vary by &gt;100-fold, with larger axons exhibiting faster conduction velocity. Axon diameter influences myelination, can be dynamically regulated, and is altered in disease. However, gaining insights into axon diameter has been challenging due to the small size and complexity of axons in vivo. Therefore, we developed an automated high-resolution live imaging and analysis screening platform to identify pharmacological modulators of the diameter of the large Mauthner axon in zebrafish. We screened 880 compounds and identified 33 hits. Validating this pipeline, we confirmed that compounds affecting beta-2 adrenoceptor and dopamine signaling increase axon diameter. This represents the first subcellular in vivo imaging-based screen to detect changes in axon diameter, providing entry points to study the biology of this key feature of neuronal morphology.<\/jats:p>","DOI":"10.1371\/journal.pbio.3003895","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:27:12Z","timestamp":1784309232000},"page":"e3003895","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["A high-resolution phenotypic screen identifies regulators of CNS axon diameter in zebrafish"],"prefix":"10.1371","volume":"24","author":[{"given":"Maria A.","family":"Eichel-Vogel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Y. H.","family":"Soong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melody N.","family":"Sequeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya L. A.","family":"Ibenfeldt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katy L. H.","family":"Marshall-Phelps","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenea M.","family":"Bin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1166-4454","authenticated-orcid":true,"given":"David A.","family":"Lyons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,7,17]]},"reference":[{"issue":"1","key":"pbio.3003895.ref001","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0896-6273(90)90444-K","article-title":"The unc-5, unc-6, and unc-40 genes guide circumferential migrations of pioneer axons and mesodermal cells on the epidermis in C. elegans","volume":"4","author":"EM Hedgecock","year":"1990","journal-title":"Neuron"},{"key":"pbio.3003895.ref002","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1242\/dev.123.1.427","article-title":"Zebrafish mutations affecting retinotectal axon pathfinding","volume":"123","author":"RO Karlstrom","year":"1996","journal-title":"Development"},{"issue":"1","key":"pbio.3003895.ref003","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0166-2236(96)40005-4","article-title":"Genetic analysis of axon guidance and mapping in the zebrafish","volume":"20","author":"RO Karlstrom","year":"1997","journal-title":"Trends Neurosci"},{"key":"pbio.3003895.ref004","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1126\/science.1072165","article-title":"Molecular mechanisms of axon guidance","volume":"298","author":"BJ Dickson","year":"2002","journal-title":"Science"},{"issue":"1","key":"pbio.3003895.ref005","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.ydbio.2006.06.021","article-title":"A genetic screen identifies genes essential for development of myelinated axons in zebrafish","volume":"298","author":"H-M Pogoda","year":"2006","journal-title":"Dev Biol"},{"key":"pbio.3003895.ref006","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1749-8104-2-9","article-title":"Drosophila as a genetic and cellular model for studies on axonal growth","volume":"2","author":"N S\u00e1nchez-Soriano","year":"2007","journal-title":"Neural Dev"},{"issue":"3","key":"pbio.3003895.ref007","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1073\/pnas.0510527104","article-title":"Axon guidance genes identified in a large-scale RNAi screen using the RNAi-hypersensitive Caenorhabditis elegans strain nre-1(hd20) lin-15b(hd126)","volume":"104","author":"C Schmitz","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"pbio.3003895.ref008","article-title":"Identification of neural outgrowth genes using genome-wide RNAi","volume":"4","author":"KJ Sepp","year":"2008","journal-title":"PLoS Genet"},{"issue":"3","key":"pbio.3003895.ref009","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1002\/dneu.20910","article-title":"Behavioral screening for neuroactive drugs in zebrafish","volume":"72","author":"J Rihel","year":"2012","journal-title":"Dev Neurobiol"},{"issue":"2","key":"pbio.3003895.ref010","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1523\/JNEUROSCI.4254-11.2012","article-title":"Why do axons differ in caliber?","volume":"32","author":"JA Perge","year":"2012","journal-title":"J Neurosci"},{"key":"pbio.3003895.ref011","article-title":"From the Physiological Laboratory, University of Cambridge","author":"WAH Rushton","year":"1951","journal-title":"J Physiol"},{"issue":"1","key":"pbio.3003895.ref012","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1113\/jphysiol.1954.sp005152","article-title":"A note on conduction velocity","volume":"125","author":"AL Hodgkin","year":"1954","journal-title":"J Physiol"},{"issue":"2","key":"pbio.3003895.ref013","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1002\/mus.880030207","article-title":"Determinants of conduction velocity in myelinated nerve fibers","volume":"3","author":"SG Waxman","year":"1980","journal-title":"Muscle Nerve"},{"issue":"6248","key":"pbio.3003895.ref014","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/342430a0","article-title":"Target size regulates calibre and myelination of sympathetic axons","volume":"342","author":"JT Voyvodic","year":"1989","journal-title":"Nature"},{"issue":"3","key":"pbio.3003895.ref015","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0092-8674(92)90183-D","article-title":"Local modulation of neurofilament phosphorylation, axonal caliber, and slow axonal transport by myelinating Schwann cells","volume":"68","author":"SM de Waegh","year":"1992","journal-title":"Cell"},{"issue":"6","key":"pbio.3003895.ref016","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1523\/JNEUROSCI.18-06-01953.1998","article-title":"Myelin-associated glycoprotein is a myelin signal that modulates the caliber of myelinated axons","volume":"18","author":"X Yin","year":"1998","journal-title":"J Neurosci"},{"issue":"1","key":"pbio.3003895.ref017","doi-asserted-by":"crossref","DOI":"10.1016\/j.cub.2006.11.042","article-title":"Rapid conduction and the evolution of giant axons and myelinated fibers","volume":"17","author":"DK Hartline","year":"2007","journal-title":"Curr Biol"},{"issue":"3","key":"pbio.3003895.ref018","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2018.09.052","article-title":"Initiation of CNS Myelination In The Optic Nerve Is Dependent On Axon Caliber","volume":"25","author":"SR Mayoral","year":"2018","journal-title":"Cell Rep"},{"key":"pbio.3003895.ref019","doi-asserted-by":"crossref","first-page":"4514","DOI":"10.1038\/s41467-020-18172-7","article-title":"CMTM6 expressed on the adaxonal Schwann cell surface restricts axonal diameters in peripheral nerves","volume":"11","author":"MA Eichel","year":"2020","journal-title":"Nat Commun"},{"issue":"1","key":"pbio.3003895.ref020","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1038\/s41467-024-45908-6","article-title":"Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons","volume":"15","author":"JM Bin","year":"2024","journal-title":"Nat Commun"},{"issue":"4","key":"pbio.3003895.ref021","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1038\/nn.3045","article-title":"Plasticity in gray and white: neuroimaging changes in brain structure during learning","volume":"15","author":"RJ Zatorre","year":"2012","journal-title":"Nat Neurosci"},{"key":"pbio.3003895.ref022","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/j.neuron.2013.10.002","article-title":"Scaling brain size, keeping timing: evolutionary preservation of brain rhythms","volume":"80","author":"G Buzs\u00e1ki","year":"2013","journal-title":"Neuron"},{"key":"pbio.3003895.ref023","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.neuroscience.2013.06.047","article-title":"Regulation of conduction time along axons","volume":"276","author":"AH Seidl","year":"2014","journal-title":"Neuroscience"},{"issue":"6","key":"pbio.3003895.ref024","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1073\/pnas.1607541114","article-title":"Superresolution imaging reveals activity-dependent plasticity of axon morphology linked to changes in action potential conduction velocity","volume":"114","author":"R Ch\u00e9reau","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"34","key":"pbio.3003895.ref025","doi-asserted-by":"crossref","first-page":"8239","DOI":"10.1523\/JNEUROSCI.3728-16.2017","article-title":"Sound-evoked activity influences myelination of brainstem axons in the trapezoid body","volume":"37","author":"JL Sinclair","year":"2017","journal-title":"J Neurosci"},{"issue":"52","key":"pbio.3003895.ref026","doi-asserted-by":"crossref","first-page":"33649","DOI":"10.1073\/pnas.2012533117","article-title":"Axon morphology is modulated by the local environment and impacts the noninvasive investigation of its structure-function relationship","volume":"117","author":"M Andersson","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"8","key":"pbio.3003895.ref027","article-title":"The axonal radial contractility: structural basis underlying a new form of neural plasticity","volume":"43","author":"X Pan","year":"2021","journal-title":"Bioessays"},{"issue":"12","key":"pbio.3003895.ref028","doi-asserted-by":"crossref","first-page":"6099","DOI":"10.1111\/ejn.15840","article-title":"Remodelling of myelinated axons and oligodendrocyte differentiation is stimulated by environmental enrichment in the young adult brain","volume":"56","author":"M Nicholson","year":"2022","journal-title":"Eur J Neurosci"},{"issue":"5","key":"pbio.3003895.ref029","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1002\/acn3.760","article-title":"Corpus callosum axon diameter relates to cognitive impairment in multiple sclerosis","volume":"6","author":"SY Huang","year":"2019","journal-title":"Ann Clin Transl Neurol"},{"key":"pbio.3003895.ref030","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1093\/brain\/123.2.308","article-title":"Axonal changes in chronic demyelinated cervical spinal cord plaques","author":"G Lovas","year":"2000","journal-title":"Brain"},{"key":"pbio.3003895.ref031","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.expneurol.2015.03.009","article-title":"Molecular regulators of nerve conduction - Lessons from inherited neuropathies and rodent genetic models","volume":"267","author":"J Li","year":"2015","journal-title":"Exp Neurol"},{"issue":"4","key":"pbio.3003895.ref032","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1002\/acn3.540","article-title":"Axonal abnormalities in vanishing white matter","volume":"5","author":"MD Klok","year":"2018","journal-title":"Ann Clin Transl Neurol"},{"issue":"1","key":"pbio.3003895.ref033","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1038\/s41398-020-00943-x","article-title":"Reduced axonal caliber and structural changes in a rat model of Fragile X syndrome with a deletion of a K-Homology domain of Fmr1","volume":"10","author":"CEM Golden","year":"2020","journal-title":"Transl Psychiatry"},{"key":"pbio.3003895.ref034","doi-asserted-by":"crossref","first-page":"147157","DOI":"10.1016\/j.brainres.2020.147157","article-title":"Atypical myelinogenesis and reduced axon caliber in the Scn1a variant model of Dravet syndrome: an electron microscopy pilot study of the developing and mature mouse corpus callosum","volume":"1751","author":"K Richards","year":"2021","journal-title":"Brain Res"},{"key":"pbio.3003895.ref035","doi-asserted-by":"crossref","first-page":"7391","DOI":"10.3390\/ijms23137391","article-title":"Long-term changes in axon calibers after injury: observations on the mouse corticospinal tract","volume":"23","author":"AS Alexandris","year":"2022","journal-title":"Int J Mol Sci"},{"key":"pbio.3003895.ref036","article-title":"Myelinated peripheral axons are more vulnerable to mechanical trauma in a model of enlarged axonal diameters","author":"VI Gargareta","year":"2024","journal-title":"Glia"},{"issue":"4","key":"pbio.3003895.ref037","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/ar.1091670402","article-title":"Axon caliber related to neurofilaments and microtubules in sciatic nerve fibers of rats and mice","volume":"167","author":"RL Friede","year":"1970","journal-title":"Anat Rec"},{"issue":"5","key":"pbio.3003895.ref038","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1083\/jcb.200308159","article-title":"NF-M is an essential target for the myelin-directed \u201coutside-in\u201d signaling cascade that mediates radial axonal growth","volume":"163","author":"ML Garcia","year":"2003","journal-title":"J Cell Biol"},{"issue":"18","key":"pbio.3003895.ref039","doi-asserted-by":"crossref","first-page":"6209","DOI":"10.1523\/JNEUROSCI.0647-12.2012","article-title":"Expansion of neurofilament medium C terminus increases axonal diameter independent of increases in conduction velocity or myelin thickness","volume":"32","author":"DM Barry","year":"2012","journal-title":"J Neurosci"},{"key":"pbio.3003895.ref040","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.celrep.2016.03.047","article-title":"The actin-binding protein \u03b1-adducin is required for maintaining axon diameter","volume":"15","author":"SC Leite","year":"2016","journal-title":"Cell Reports"},{"key":"pbio.3003895.ref041","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.55471","article-title":"The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction","volume":"9","author":"AR Costa","year":"2020","journal-title":"Elife"},{"key":"pbio.3003895.ref042","article-title":"Neurofilaments are spaced randomly in the radial dimension of axons","author":"RL Price","year":"1988","journal-title":"J Neurocytol"},{"key":"pbio.3003895.ref043","doi-asserted-by":"crossref","first-page":"319","DOI":"10.3389\/fnmol.2018.00319","article-title":"The regulation of axon diameter: from axonal circumferential contractility to activity-dependent axon swelling","volume":"11","author":"AR Costa","year":"2018","journal-title":"Front Mol Neurosci"},{"key":"pbio.3003895.ref044","author":"JM Bin","year":"2025"},{"issue":"2","key":"pbio.3003895.ref045","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/cne.902050203","article-title":"Brain neurons which project to the spinal cord in young larvae of the zebrafish","volume":"205","author":"CB Kimmel","year":"1982","journal-title":"J Comp Neurol"},{"key":"pbio.3003895.ref046","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/cne.902510202","article-title":"Segmental homologies among reticulospinal neurons in the hindbrain of the zebrafish larva","volume":"251","author":"WK Metcalfe","year":"1986","journal-title":"J Comp Neurol"},{"issue":"9","key":"pbio.3003895.ref047","doi-asserted-by":"crossref","DOI":"10.1016\/j.cub.2009.02.025","article-title":"Mauthner cells","volume":"19","author":"KT Sillar","year":"2009","journal-title":"Curr Biol"},{"key":"pbio.3003895.ref048","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.35136","article-title":"An automated high-resolution in vivo screen in zebrafish to identify chemical regulators of myelination","volume":"7","author":"JJ Early","year":"2018","journal-title":"Elife"},{"issue":"4","key":"pbio.3003895.ref049","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1073\/pnas.0704963105","article-title":"Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish","volume":"105","author":"K Asakawa","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"pbio.3003895.ref050","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1523\/JNEUROSCI.4814-11.2012","article-title":"Arrest of myelination and reduced axon growth when Schwann cells lack mTOR","volume":"32","author":"DL Sherman","year":"2012","journal-title":"J Neurosci"},{"issue":"44","key":"pbio.3003895.ref051","doi-asserted-by":"crossref","first-page":"14861","DOI":"10.1523\/JNEUROSCI.4968-14.2015","article-title":"Fbxw7 limits myelination by inhibiting mTOR signaling","volume":"35","author":"CA Kearns","year":"2015","journal-title":"J Neurosci"},{"issue":"1","key":"pbio.3003895.ref052","doi-asserted-by":"crossref","DOI":"10.1002\/dvg.23340","article-title":"Creation of zebrafish knock-in reporter lines in the nefma gene by Cas9-mediated homologous recombination","volume":"58","author":"A Eschstruth","year":"2020","journal-title":"Genesis"},{"issue":"6","key":"pbio.3003895.ref053","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1242\/dmm.018689","article-title":"Phenotype-driven chemical screening in zebrafish for compounds that inhibit collective cell migration identifies multiple pathways potentially involved in metastatic invasion","volume":"8","author":"VE Gallardo","year":"2015","journal-title":"Dis Model Mech"},{"issue":"1","key":"pbio.3003895.ref054","doi-asserted-by":"crossref","first-page":"9955","DOI":"10.1038\/s41467-024-54375-y","article-title":"Deep phenotypic profiling of neuroactive drugs in larval zebrafish","volume":"15","author":"L Gendelev","year":"2024","journal-title":"Nat Commun"},{"issue":"7","key":"pbio.3003895.ref055","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1177\/1087057111405851","article-title":"Illustration of SSMD, z score, SSMD*, z* score, and t statistic for hit selection in RNAi high-throughput screens","volume":"16","author":"XD Zhang","year":"2011","journal-title":"J Biomol Screen"},{"key":"pbio.3003895.ref056","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.1038\/ncomms3410","article-title":"Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment","volume":"4","author":"SC Baraban","year":"2013","journal-title":"Nat Commun"},{"issue":"10","key":"pbio.3003895.ref057","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1002\/glia.23166","article-title":"Drug discovery for remyelination and treatment of MS","volume":"65","author":"KLH Cole","year":"2017","journal-title":"Glia"},{"issue":"7","key":"pbio.3003895.ref058","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.202216267","article-title":"A multilevel screening pipeline in zebrafish identifies therapeutic drugs for GAN","volume":"15","author":"L Lescouz\u00e8res","year":"2023","journal-title":"EMBO Mol Med"},{"issue":"12","key":"pbio.3003895.ref059","doi-asserted-by":"crossref","DOI":"10.1242\/dev.201323","article-title":"Fast, precise and cloning-free knock-in of reporter sequences in vivo with high efficiency","volume":"150","author":"Y Zhang","year":"2023","journal-title":"Development"},{"issue":"14","key":"pbio.3003895.ref060","doi-asserted-by":"crossref","first-page":"5512","DOI":"10.1523\/JNEUROSCI.5548-10.2011","article-title":"Dopamine D2 receptor activity modulates Akt signaling and alters GABAergic neuron development and motor behavior in zebrafish larvae","volume":"31","author":"BR Souza","year":"2011","journal-title":"J Neurosci"},{"issue":"4","key":"pbio.3003895.ref061","doi-asserted-by":"crossref","DOI":"10.1111\/gbb.12460","article-title":"Domperidone upregulates dopamine receptor expression and stimulates locomotor activity in larval zebrafish (Danio rerio)","volume":"17","author":"EC Shontz","year":"2018","journal-title":"Genes Brain Behav"},{"issue":"3","key":"pbio.3003895.ref062","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1007\/s12264-019-00431-3","article-title":"Dopamine D2 receptor-mediated modulation of rat retinal ganglion cell excitability","volume":"36","author":"N Yin","year":"2020","journal-title":"Neurosci Bull"},{"issue":"4","key":"pbio.3003895.ref063","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/j.neuron.2012.05.035","article-title":"Visual input modulates audiomotor function via hypothalamic dopaminergic neurons through a cooperative mechanism","volume":"75","author":"Y Mu","year":"2012","journal-title":"Neuron"},{"issue":"9","key":"pbio.3003895.ref064","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1002\/cne.22278","article-title":"Regional distribution and cellular localization of beta2-adrenoceptors in the adult zebrafish brain (Danio rerio)","volume":"518","author":"K Ampatzis","year":"2010","journal-title":"J Comp Neurol"},{"issue":"5","key":"pbio.3003895.ref065","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1101\/lm.030411.113","article-title":"Activation of \u03b22-adrenoceptor enhances synaptic potentiation and behavioral memory via cAMP-PKA signaling in the medial prefrontal cortex of rats","volume":"20","author":"H-C Zhou","year":"2013","journal-title":"Learn Mem"},{"key":"pbio.3003895.ref066","article-title":"Lack of \u03b22-AR increases anxiety-like behaviors and rewarding properties of cocaine","volume":"11","author":"H Zhu","year":"2017","journal-title":"Front Behav Neurosci"},{"key":"pbio.3003895.ref067","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1242\/jeb.052803","article-title":"In vivo and in vitro assessment of cardiac beta-adrenergic receptors in larval zebrafish (Danio rerio)","volume":"214","author":"SL Steele","year":"2011","journal-title":"J Exp Biol"},{"key":"pbio.3003895.ref068","doi-asserted-by":"crossref","first-page":"109039","DOI":"10.1016\/j.biopha.2019.109039","article-title":"Detailed insight on \u03b2-adrenoceptors as therapeutic targets","volume":"117","author":"BK Velmurugan","year":"2019","journal-title":"Biomed Pharmacother"},{"issue":"8","key":"pbio.3003895.ref069","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.tig.2022.04.007","article-title":"Conditional mutagenesis strategies in zebrafish","volume":"38","author":"M Kalvaityt\u0117","year":"2022","journal-title":"Trends Genet"},{"key":"pbio.3003895.ref070","article-title":"Cre\/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration","volume":"11","author":"F Liu","year":"2022","journal-title":"Elife"},{"issue":"17","key":"pbio.3003895.ref071","doi-asserted-by":"crossref","first-page":"3757","DOI":"10.1242\/dev.126.17.3757","article-title":"nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate","volume":"126","author":"JA Lister","year":"1999","journal-title":"Development"},{"issue":"3","key":"pbio.3003895.ref072","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/gtc.12032","article-title":"Glycinergic transmission and postsynaptic activation of CaMKII are required for glycine receptor clustering in vivo","volume":"18","author":"I Yamanaka","year":"2013","journal-title":"Genes Cells"}],"container-title":["PLOS Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pbio.3003895","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:27:22Z","timestamp":1784309242000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pbio.3003895"}},"subtitle":[],"editor":[{"given":"Cody J.","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,7,17]]},"references-count":72,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pbio.3003895","relation":{},"ISSN":["1545-7885"],"issn-type":[{"value":"1545-7885","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,17]]}}}