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How, exactly, coronin proteins exert their function is debated. On the one hand, coronins are widely considered as F-actin-binding and regulatory proteins. On the other hand, coronin proteins were also shown to act as regulators of cAMP\/Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling. Here, we demonstrate that endogenously expressed mammalian coronin 1, 2 and 3 did not depend on F-actin for their subcellular localization and did not impact actin-related processes that were previously reported to be coronin-dependent. We also show that the fusion of GFP or FLAG-tags to coronin proteins resulted in their increased colocalization with actin and phenocopied loss-of-function mutation in mice. Together these results suggest that inclusion of tags on coronin proteins causes increased colocalization with F-actin and can disrupt their in vivo function. Furthermore, we show that the ubiquitously expressed coronin 2 and coronin 3 regulated cAMP production regardless of the presence of F-actin. The analysis presented here may contribute to a re-evaluation of the function of coronin proteins in signaling, independently of actin modulation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pbio.3003904","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T17:42:42Z","timestamp":1787074962000},"page":"e3003904","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reassessment of the roles of coronin proteins as actin effectors and in signaling"],"prefix":"10.1371","volume":"24","author":[{"given":"Roko","family":"Gvozdenica 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