{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T18:11:06Z","timestamp":1788977466140,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.pbio.3003969","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000}}],"reference-count":93,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["PO 523\/10-1 project number 538832008"],"award-info":[{"award-number":["PO 523\/10-1 project number 538832008"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["INST 186\/1230-1 FUGG"],"award-info":[{"award-number":["INST 186\/1230-1 FUGG"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["INST 186\/1465-1"],"award-info":[{"award-number":["INST 186\/1465-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosbiology.org"],"crossmark-restriction":false},"short-container-title":["PLoS Biol"],"abstract":"<jats:p>\n                    Hyphal fusion and sexual development in filamentous fungi rely on coordinated signaling of numerous conserved nodes such as the striatin-interacting phosphatase and kinase (STRIPAK) complex or the pheromone response (PR) MAP kinase cascade (MIK2, MEK2, MAK2, HAM5). Here, we used the homothallic ascomycete\n                    <jats:italic>Sordaria macrospora<\/jats:italic>\n                    (Sm) to screen for putative protein interactors of the SmSTRIPAK complex. Using the STRIPAK complex interactor 1 (SCI1) subunit of the complex as bait, we enriched and identified canonical SmSTRIPAK components and a determinant of communication (DOC) protein. The DOC proteins were previously described in the closely related and heterothallic species\n                    <jats:italic>Neurospora crassa<\/jats:italic>\n                    , functioning in allorecognition of germlings and hyphal fusions. We generated \u0394Smdoc1, \u0394Smdoc2 single-deletion strains and the double deletion mutant \u0394Smdoc1\u0394Smdoc2 in\n                    <jats:italic>S. macrospora<\/jats:italic>\n                    . Deletion phenotypes were paradoxical: single knockouts (\u0394Smdoc1 or \u0394Smdoc2) were nearly sterile, and sexual development was impaired, yet the double mutant (\u0394Smdoc1\u0394Smdoc2) exhibited wild-type fertility and development, demonstrating non-redundant and mutually antagonistic roles. Using gene tagging at the native locus, we performed TurboID-based proximity mapping with SmDOC1 and SmDOC2 as bait proteins. This proximity mapping demonstrated close ties of SmDOC1\/2 to components of the PR MAP kinase pathway and revealed mutual SmDOC1 \u2013 SmDOC2 proximity. Yeast two-hybrid experiments with SmDOC1 confirmed the direct interaction with the MAP kinases MEK2 and MAK2. Fluorescence microscopy revealed that SmDOC1-TagRFP-T localized to structures near septal pores. Our results demonstrate that the DOC system is not restricted to heterothallic\n                    <jats:italic>N. crassa<\/jats:italic>\n                    but also plays an essential role in the development of fruiting bodies in the homothallic fungus\n                    <jats:italic>S. macrospora<\/jats:italic>\n                    . These findings suggest the DOC1\/2 proteins as a novel system that integrates STRIPAK and PR pathways, providing a possible mechanistic explanation for their non-additive deletion strain phenotypes.\n                  <\/jats:p>","DOI":"10.1371\/journal.pbio.3003969","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T17:46:46Z","timestamp":1787852806000},"page":"e3003969","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["STRIPAK-associated greenbeard proteins DOC1 and DOC2 regulate MAK2 signaling and sexual development in Sordaria macrospora"],"prefix":"10.1371","volume":"24","author":[{"given":"Lucas S.","family":"Hollstein","sequence":"first","affiliation":[{"name":"Institute of Microbiology and Genetics, University of G\u00f6ttingen","place":["G\u00f6ttingen, Germany"],"department":["Department of Genetics of Eukaryotic 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