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Yet this powerful approach remains largely confined to solanaceous species, leaving most economically important crop families without a comparable rapid assay platform. Here, we show that an engineered\n                    <jats:italic>Rhizobium rhizogenes<\/jats:italic>\n                    strain, AS109, mediates efficient transient expression across diverse dicot species spanning multiple taxonomic families, consistently outperforming commonly used laboratory agrobacterial strains. Leveraging the broad host range of AS109, we establish a suite of functional assays in faba bean (\n                    <jats:italic>Vicia faba<\/jats:italic>\n                    ), including protein localisation, RNA interference-mediated gene silencing, cell-surface elicitor recognition screens, nucleotide-binding leucine-rich repeat receptor (NLR) activation, and infection cell biology at the host-pathogen interface. We further demonstrate that both singleton NLRs and sensor-helper NLR pairs from Solanaceae retain effector recognition and cell death activity when transferred into faba bean, establishing a rapid platform for evaluating cross-family transferability of disease-resistance genes. AS109 thus provides an accessible and versatile chassis for functional genomics in non-model crops, bridging the widening gap between hypothesis generation and experimental validation across diverse plant species.\n                  <\/jats:p>","DOI":"10.1371\/journal.pbio.3004010","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:22:21Z","timestamp":1792084941000},"page":"e3004010","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["A broad-host-range Rhizobium rhizogenes strain enables transient expression across diverse crop species"],"prefix":"10.1371","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6377-4244","authenticated-orcid":true,"given":"Freddie","family":"King","sequence":"first","affiliation":[{"name":"Imperial College London","place":["London, United Kingdom"],"department":["Department of Life 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