{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T18:48:42Z","timestamp":1782499722621,"version":"3.54.5"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1013928","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000}}],"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013404","name":"Universidad EAFIT","doi-asserted-by":"publisher","award":["Vicerrector\u00eda de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n"],"award-info":[{"award-number":["Vicerrector\u00eda de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n"]}],"id":[{"id":"10.13039\/100013404","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2404769"],"award-info":[{"award-number":["2404769"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>\n                    Holographic imaging in microscopy enables label-free quantitative information of biological specimens and has found applications across a wide range of biomedical studies, from cell morphology to particle dynamics; yet its widespread adoption is often limited by the lack of accessible and standardized analysis software. We present\n                    <jats:italic>HoloBio<\/jats:italic>\n                    , an open-source, Python-based graphical user interface developed to address this issue. This software offers two primary operational modes: a\n                    <jats:italic>Real-Time<\/jats:italic>\n                    mode that enables live processing of holograms at video frame rates, and an\n                    <jats:italic>Offline<\/jats:italic>\n                    mode designed for post-processing previously recorded holograms.\n                    <jats:italic>HoloBio<\/jats:italic>\n                    is compatible with holograms recorded using both lens-based and lensless systems, supporting off-axis architectures in telecentric and non-telecentric configurations, as well as slightly off-axis and in-line optical setups. The software incorporates tools for cell tracking, phase profiling, thickness estimation, and morphological analysis, including cell counting and object area quantification.\n                    <jats:italic>HoloBio<\/jats:italic>\n                    is designed to be accessible for users without coding expertise, offering a reproducible, high-throughput environment tailored for researchers in biology, biophotonics, and biomedical imaging.\n                  <\/jats:p>","DOI":"10.1371\/journal.pcbi.1013928","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T17:42:12Z","timestamp":1782150132000},"page":"e1013928","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["HoloBio: A holographic microscopy tool for quantitative biological analysis"],"prefix":"10.1371","volume":"22","author":[{"given":"Waira","family":"Mona","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"Gil-Herrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuel","family":"Mazo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"C\u00f3rdoba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Obando-Vasquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"Lopera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3874-3032","authenticated-orcid":true,"given":"Rene","family":"Restrepo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Trujillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0448-376X","authenticated-orcid":true,"given":"Ana","family":"Doblas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1546-4049","authenticated-orcid":true,"given":"Raul","family":"Castaneda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"pcbi.1013928.ref001","first-page":"18005","article-title":"Principles and techniques of digital holographic microscopy","volume":"1","author":"MK Kim","year":"2010","journal-title":"SPIE Rev"},{"key":"pcbi.1013928.ref002","first-page":"219","article-title":"Label-free quantitative in vitro live cell imaging with digital holographic microscopy.","author":"J Wegener","year":"2019","journal-title":"Label-free monitoring of cells in vitro. 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