{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T18:12:05Z","timestamp":1781115125732,"version":"3.54.1"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1014342","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000}}],"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC 2047\u2014390685813"],"award-info":[{"award-number":["EXC 2047\u2014390685813"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC 2151\u2014390873048"],"award-info":[{"award-number":["EXC 2151\u2014390873048"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["GA number 945700"],"award-info":[{"award-number":["GA number 945700"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014604","name":"Mathematical Institute of the University of Bonn","doi-asserted-by":"publisher","award":["Schlegel Professorship"],"award-info":[{"award-number":["Schlegel Professorship"]}],"id":[{"id":"10.13039\/501100014604","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Institute for Experimental Epileptology and Cognition Research"}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>\n                    RNA-binding proteins (RBP) are important regulators of RNA metabolism. In neurodegenerative disorders such as Huntington\u2019s Disease (HD), disrupted RBP-RNA interactions contribute to neuronal dysfunction. One such RBP, Midline 1 (MID1), has been shown to aberrantly associate with mutant huntingtin (\n                    <jats:italic>Htt<\/jats:italic>\n                    ) RNA, enhancing its translation, yet the mechanism driving this effect remains unknown. Here, we develop a computational model to understand the role of MID1. Based on previously published data, our model predicts that MID1 increases the stability of the\n                    <jats:italic>Htt<\/jats:italic>\n                    RNA. We experimentally validate this prediction, showing that overexpression of MID1 significantly prolongs the half-life of mutant\n                    <jats:italic>Htt<\/jats:italic>\n                    RNA. Furthermore, we evaluate model refinements, including clustering of MID1-bound RNA, which allow capturing all key observations in the data. Together, we provide a data-driven framework that underlines the importance of RBP-RNA interaction in post-transcriptional regulation. This framework also shows how individual molecular reactions jointly determine RNA stability and protein levels in HD.\n                  <\/jats:p>","DOI":"10.1371\/journal.pcbi.1014342","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T17:41:01Z","timestamp":1780422061000},"page":"e1014342","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Data-driven model reveals increased stability of CAG-expanded huntingtin RNA due to MID1 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