{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T18:03:54Z","timestamp":1784916234946,"version":"3.55.0"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1014464","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000}}],"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 81930019"],"award-info":[{"award-number":["No. 81930019"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Scientific Project of Beijing\u2019s Municipal Science & Technology Commission","award":["D171100002817005"],"award-info":[{"award-number":["D171100002817005"]}]},{"name":"Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support Programme","award":["ZYLX201823"],"award-info":[{"award-number":["ZYLX201823"]}]},{"name":"Training Fund for Open Projects at Clinical Institutes and Departments of Capital Medical University","award":["CCMU2024ZKYXY005"],"award-info":[{"award-number":["CCMU2024ZKYXY005"]}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>Cathepsin L (CTSL) is a prominent therapeutic target for kidney injury, yet clinically available CTSL inhibitors remain limited. Here, we developed an artificial intelligence (AI)-assisted discovery strategy to identify novel CTSL inhibitors from a natural products library. Through a robust deep learning model and molecular docking, we screened 200 molecules from natural products library for experimental validation. Active candidates were further analyzed by molecular dynamics simulations to characterize binding modes and key CTSL-ligand interaction networks, followed by evaluation of therapeutic efficacy in kidney injury-relevant models. At a concentration of 100 \u00b5M, we found that 43 of them exhibited more than 50% inhibition of CTSL. Notably, nine molecules displayed over 90% inhibition and exhibited concentration-dependent effects. Molecular dynamics simulations indicated that Kuwanon G (KG), Iberverin, and Wighteone stably bind within the CTSL active site. In human renal cells, KG attenuated high glucose and high lipid induced inflammatory and injury responses. Collectively, these findings identify new CTSL inhibitors with therapeutic potential for renal injury and underscore the utility of AI-assisted strategies in accelerating drug discovery.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1014464","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T17:58:02Z","timestamp":1783706282000},"page":"e1014464","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["AI-guided identification of natural CTSL inhibitors with therapeutic potential for renal 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