{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:08:56Z","timestamp":1785434936385,"version":"3.56.0"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1014573","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000}}],"reference-count":71,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"crossref","award":["ZR2024MH032"],"award-info":[{"award-number":["ZR2024MH032"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Postdoctoral Innovation Program of Shandong Province","award":["SDCX-ZG-202503112"],"award-info":[{"award-number":["SDCX-ZG-202503112"]}]},{"name":"Major Basic Research Project of the Shandong Provincial Natural Science Foundation","award":["ZR2024ZD13"],"award-info":[{"award-number":["ZR2024ZD13"]}]},{"name":"Major Basic Research Project of the Shandong Provincial Natural Science Foundation","award":["ZR2023ZD16"],"award-info":[{"award-number":["ZR2023ZD16"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82372413"],"award-info":[{"award-number":["82372413"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82220108005"],"award-info":[{"award-number":["82220108005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Taishan Scholars Program of Shandong Province-Pandeng Taishan Scholars","award":["tspd20210320"],"award-info":[{"award-number":["tspd20210320"]}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>\n                    m\n                    <jats:sup>1<\/jats:sup>\n                    A (N1-methyladenosine) is an important epigenetic mechanism that regulates the onset and progression of many diseases, including spinal cord injury (SCI). To investigate the overall changes in m\n                    <jats:sup>1<\/jats:sup>\n                    A following SCI, we analyzed transcriptomic sequencing data from SCI samples and assigned m\n                    <jats:sup>1<\/jats:sup>\n                    A scores based on the levels of m\n                    <jats:sup>1<\/jats:sup>\n                    A regulatory factors. In this study, the m\n                    <jats:sup>1<\/jats:sup>\n                    A score is an inferred proxy calculated from the expression of m\n                    <jats:sup>1<\/jats:sup>\n                    A regulator genes (writers\/erasers\/readers). It does not directly measure RNA m\n                    <jats:sup>1<\/jats:sup>\n                    A modification levels. Our results show that the m\n                    <jats:sup>1<\/jats:sup>\n                    A score increased within the first day after SCI and then decreased, falling below baseline by day 3 and day 7. Further analysis revealed that microglia and neurons are the two cell types with the most significant changes in the m\n                    <jats:sup>1<\/jats:sup>\n                    A score. In microglia, m\n                    <jats:sup>1<\/jats:sup>\n                    A score decreased at all time points, whereas in neurons, m\n                    <jats:sup>1<\/jats:sup>\n                    A score increased at all time points. Additionally, pseudotime and functional enrichment analyses suggested that the m\n                    <jats:sup>1<\/jats:sup>\n                    A score is associated with microglial phenotypic transition and neuronal energy metabolism, which was further validated by both in vivo and in vitro experiments. In summary, our study unveils the characteristic changes of m\n                    <jats:sup>1<\/jats:sup>\n                    A at both the bulk and single-cell levels following SCI, and suggests potential links to neuronal function and supports the rationale for further studies exploring m\n                    <jats:sup>1<\/jats:sup>\n                    A-related regulators as therapeutic targets in SCI.\n                  <\/jats:p>","DOI":"10.1371\/journal.pcbi.1014573","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T17:46:12Z","timestamp":1784915172000},"page":"e1014573","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cell-type-specific m1A dynamics are associated with microglial phenotypic transition and neuronal metabolic adaptation during spinal cord injury"],"prefix":"10.1371","volume":"22","author":[{"given":"Chi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaolong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruizhi","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enlin","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqing","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0053-8970","authenticated-orcid":true,"given":"Hengxing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"issue":"5","key":"pcbi.1014573.ref001","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/j.suc.2017.06.008","article-title":"Trauma: 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