{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T12:27:23Z","timestamp":1780057643421,"version":"3.54.0"},"reference-count":64,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"DOI":"10.1371\/journal.pgen.1001255","type":"journal-article","created":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T21:36:21Z","timestamp":1293140181000},"page":"e1001255","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":78,"title":["A Young Drosophila Duplicate Gene Plays Essential Roles in Spermatogenesis by Regulating Several Y-Linked Male Fertility Genes"],"prefix":"10.1371","volume":"6","author":[{"given":"Yun","family":"Ding","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoping","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guojie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijing","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"ref1","article-title":"Evolution by gene duplication.","author":"S Ohno","year":"1970"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/S1673-8527(08)60085-5","article-title":"On the origin and evolution of new genes\u2013a genomic and experimental perspective.","volume":"35","author":"Q Zhou","year":"2008","journal-title":"J Genet Genomics"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1038\/nrg1204","article-title":"The origin of new genes: glimpses from the young and old.","volume":"4","author":"M Long","year":"2003","journal-title":"Nat Rev Genet"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1093\/genetics\/154.1.459","article-title":"The probability of duplicate gene preservation by subfunctionalization.","volume":"154","author":"M Lynch","year":"2000","journal-title":"Genetics"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1126\/science.290.5494.1151","article-title":"The evolutionary fate and consequences of duplicate genes.","volume":"290","author":"M Lynch","year":"2000","journal-title":"Science"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.cub.2004.12.071","article-title":"Origin and neofunctionalization of a Drosophila paternal effect gene essential for zygote viability.","volume":"15","author":"B Loppin","year":"2005","journal-title":"Curr Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1093\/molbev\/msl201","article-title":"Drosophila mojoless, a retroposed GSK-3, has functionally diverged to acquire an essential role in male fertility.","volume":"24","author":"R Kalamegham","year":"2007","journal-title":"Mol Biol Evol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"7478","DOI":"10.1073\/pnas.0800693105","article-title":"The evolution of courtship behaviors through the origination of a new gene in Drosophila.","volume":"105","author":"H Dai","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1038\/cr.2010.31","article-title":"A de novo originated gene depresses budding yeast mating pathway and is repressed by the protein encoded by its antisense strand.","volume":"20","author":"D Li","year":"2010","journal-title":"Cell Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"10943","DOI":"10.1073\/pnas.1006503107","article-title":"Adaptive impact of the chimeric gene Quetzalcoatl in Drosophila melanogaster.","volume":"107","author":"RL Rogers","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"16246","DOI":"10.1073\/pnas.0407066101","article-title":"Evolving protein functional diversity in new genes of Drosophila.","volume":"101","author":"J Zhang","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1038\/25126","article-title":"Selective sweep of a newly evolved sperm-specific gene in Drosophila.","volume":"396","author":"DI Nurminsky","year":"1998","journal-title":"Nature"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/ng852","article-title":"Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leaf-eating monkey.","volume":"30","author":"J Zhang","year":"2002","journal-title":"Nat Genet"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"4448","DOI":"10.1073\/pnas.072066399","article-title":"Origin of sphinx, a young chimeric RNA gene in Drosophila melanogaster.","volume":"99","author":"W Wang","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.1371\/journal.pgen.0040003","article-title":"Repetitive element-mediated recombination as a mechanism for new gene origination in Drosophila.","volume":"4","author":"S Yang","year":"2008","journal-title":"PLoS Genet"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1073\/pnas.0236048100","article-title":"Kep1 interacts genetically with dredd\/caspase-8, and kep1 mutants alter the balance of dredd isoforms.","volume":"100","author":"M Di Fruscio","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1093\/oxfordjournals.molbev.a025957","article-title":"Likelihood ratio tests for detecting positive selection and application to primate lysozyme evolution.","volume":"15","author":"Z Yang","year":"1998","journal-title":"Mol Biol Evol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1093\/molbev\/msg236","article-title":"Temporal patterns of fruit fly (Drosophila) evolution revealed by mutation clocks.","volume":"21","author":"K Tamura","year":"2004","journal-title":"Mol Biol Evol"},{"key":"ref19","article-title":"Molecular Evoltuion.","author":"W Li","year":"1997"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1093\/genetics\/147.1.199","article-title":"Polymorphism and divergence at a Drosophila pseudogene locus.","volume":"147","author":"JK Pritchard","year":"1997","journal-title":"Genetics"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1101\/gr.6249707","article-title":"Evolutionary rate analyses of orthologs and paralogs from 12 Drosophila genomes.","volume":"17","author":"A Heger","year":"2007","journal-title":"Genome Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"30122","DOI":"10.1074\/jbc.273.46.30122","article-title":"The identification of two Drosophila K homology domain proteins. Kep1 and SAM are members of the Sam68 family of GSG domain proteins.","volume":"273","author":"M Di Fruscio","year":"1998","journal-title":"J Biol Chem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e1000731","DOI":"10.1371\/journal.pgen.1000731","article-title":"Stage-specific expression profiling of Drosophila spermatogenesis suggests that meiotic sex chromosome inactivation drives genomic relocation of testis-expressed genes.","volume":"5","author":"MD Vibranovski","year":"2009","journal-title":"PLoS Genet"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1038\/nrm1172","article-title":"Nuclear speckles: a model for nuclear organelles.","volume":"4","author":"AI Lamond","year":"2003","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/0955-0674(93)90009-F","article-title":"Nuclear organization of pre-mRNA processing.","volume":"5","author":"DL Spector","year":"1993","journal-title":"Curr Opin Cell Biol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.tree.2007.05.002","article-title":"Protein subcellular relocalization: a new perspective on the origin of novel genes.","volume":"22","author":"SA Byun-McKay","year":"2007","journal-title":"Trends Ecol Evol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1534\/genetics.104.030882","article-title":"Gene deletions by ends-in targeting in Drosophila melanogaster.","volume":"168","author":"HB Xie","year":"2004","journal-title":"Genetics"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1093\/genetics\/118.3.461","article-title":"A stable genomic source of P element transposase in Drosophila melanogaster.","volume":"118","author":"HM Robertson","year":"1988","journal-title":"Genetics"},{"key":"ref29","first-page":"225","article-title":"Spermatogensis.","author":"DL Lindsley","year":"1980"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1242\/dev.125.10.1833","article-title":"Genetic dissection of sperm individualization in Drosophila melanogaster.","volume":"125","author":"JJ Fabrizio","year":"1998","journal-title":"Development"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0925-4773(97)00031-2","article-title":"The Drosophila don juan (dj) gene encodes a novel sperm specific protein component characterized by an unusual domain of a repetitive amino acid motif.","volume":"64","author":"A Santel","year":"1997","journal-title":"Mech Dev"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nrg2484","article-title":"RNA-Seq: a revolutionary tool for transcriptomics.","volume":"10","author":"Z Wang","year":"2009","journal-title":"Nat Rev Genet"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"7405","DOI":"10.1073\/pnas.79.23.7405","article-title":"Structural genes on the Y chromosome of Drosophila melanogaster.","volume":"79","author":"LS Goldstein","year":"1982","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"11132","DOI":"10.1073\/pnas.90.23.11132","article-title":"A fertility region on the Y chromosome of Drosophila melanogaster encodes a dynein microtubule motor.","volume":"90","author":"J Gepner","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"13239","DOI":"10.1073\/pnas.230438397","article-title":"Y chromosomal fertility factors kl-2 and kl-3 of Drosophila melanogaster encode dynein heavy chain polypeptides.","volume":"97","author":"AB Carvalho","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1093\/genetics\/107.4.591","article-title":"Cytogenetic analysis of a segment of the Y chromosome of Drosophila melanogaster.","volume":"107","author":"RW Hardy","year":"1984","journal-title":"Genetics"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1007\/BF00328522","article-title":"Analysis of spermatogenesis in Drosophila melanogaster bearing deletions for Y-chromosome fertility genes.","volume":"83","author":"RW Hardy","year":"1981","journal-title":"Chromosoma"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1093\/genetics\/150.2.735","article-title":"Y-Linked male sterile mutations induced by P element in Drosophila melanogaster.","volume":"150","author":"P Zhang","year":"1998","journal-title":"Genetics"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1093\/genetics\/155.1.179","article-title":"Genetic analysis of a Y-chromosome region that induces triplosterile phenotypes and is essential for spermatid individualization in Drosophila melanogaster.","volume":"155","author":"B Timakov","year":"2000","journal-title":"Genetics"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1128\/MMBR.63.2.405-445.1999","article-title":"Formation of mRNA 3\u2032 ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.","volume":"63","author":"J Zhao","year":"1999","journal-title":"Microbiol Mol Biol Rev"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/0968-0004(91)90088-D","article-title":"RNA recognition: towards identifying determinants of specificity.","volume":"16","author":"DJ Kenan","year":"1991","journal-title":"Trends Biochem Sci"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1038\/nsmb961","article-title":"Building specificity with nonspecific RNA-binding proteins.","volume":"12","author":"R Singh","year":"2005","journal-title":"Nat Struct Mol Biol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"e357","DOI":"10.1371\/journal.pbio.0030357","article-title":"Emergence of young human genes after a burst of retroposition in primates.","volume":"3","author":"AC Marques","year":"2005","journal-title":"PLoS Biol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"3220","DOI":"10.1073\/pnas.0511307103","article-title":"Evolutionary fate of retroposed gene copies in the human genome.","volume":"103","author":"N Vinckenbosch","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1038\/nature06323","article-title":"Constraint and turnover in sex-biased gene expression in the genus Drosophila.","volume":"450","author":"Y Zhang","year":"2007","journal-title":"Nature"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/nrg2487","article-title":"RNA-based gene duplication: mechanistic and evolutionary insights.","volume":"10","author":"H Kaessmann","year":"2009","journal-title":"Nat Rev Genet"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1101\/gr.088609.108","article-title":"General gene movement off the X chromosome in the Drosophila genus.","volume":"19","author":"MD Vibranovski","year":"2009","journal-title":"Genome Res"},{"key":"ref48","first-page":"1177","article-title":"The cytology of testis.","author":"DM de Krester","year":"1994"},{"key":"ref49","first-page":"67","article-title":"Motility of human sperm without outer dynein arms.","volume":"15","author":"P Jouannet","year":"1983","journal-title":"J Submicrosc Cytol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1530\/jrf.0.0970487","article-title":"Pregnancy after subzonal insemination with spermatozoa lacking outer dynein arms.","volume":"97","author":"JP Wolf","year":"1993","journal-title":"J Reprod Fertil"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1530\/jrf.0.1100071","article-title":"Investigation of the association between the presence of cytoplasmic residues on the human sperm midpiece and defective sperm function.","volume":"110","author":"J Keating","year":"1997","journal-title":"J Reprod Fertil"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1093\/bib\/5.2.150","article-title":"MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment.","volume":"5","author":"S Kumar","year":"2004","journal-title":"Brief Bioinform"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1093\/oxfordjournals.molbev.a026236","article-title":"Estimating synonymous and nonsynonymous substitution rates under realistic evolutionary models.","volume":"17","author":"Z Yang","year":"2000","journal-title":"Mol Biol Evol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1126\/science.6289436","article-title":"Genetic transformation of Drosophila with transposable element vectors.","volume":"218","author":"GM Rubin","year":"1982","journal-title":"Science"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"726, 728, 730, 732","DOI":"10.2144\/00294bm10","article-title":"GFP and beta-galactosidase transformation vectors for promoter\/enhancer analysis in Drosophila.","volume":"29","author":"S Barolo","year":"2000","journal-title":"Biotechniques"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1006\/meth.2001.1183","article-title":"The tandem affinity purification (TAP) method: a general procedure of protein complex purification.","volume":"24","author":"O Puig","year":"2001","journal-title":"Methods"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"2853","DOI":"10.1242\/dev.00510","article-title":"Requirement for two nearly identical TGIF-related homeobox genes in Drosophila spermatogenesis.","volume":"130","author":"Z Wang","year":"2003","journal-title":"Development"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/BF02524646","article-title":"Association of RB97D, an RRM protein required for male fertility, with a Y chromosome lampbrush loop in Drosophila spermatocytes.","volume":"105","author":"VM Heatwole","year":"1996","journal-title":"Chromosoma"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"5116","DOI":"10.1073\/pnas.091062498","article-title":"Significance analysis of microarrays applied to the ionizing radiation response.","volume":"98","author":"VG Tusher","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.1093\/bioinformatics\/btp336","article-title":"SOAP2: an improved ultrafast tool for short read alignment.","volume":"25","author":"R Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1093\/bioinformatics\/btp612","article-title":"DEGseq: an R package for identifying differentially expressed genes from RNA-seq data.","volume":"26","author":"L Wang","year":"2010","journal-title":"Bioinformatics"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/nmeth.1226","article-title":"Mapping and quantifying mammalian transcriptomes by RNA-Seq.","volume":"5","author":"A Mortazavi","year":"2008","journal-title":"Nat Methods"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1242\/jcs.6.1.177","article-title":"Development, organization, and degeneration of the Drosophila sperm flagellum.","volume":"6","author":"BI Kiefer","year":"1970","journal-title":"J Cell Sci"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/463308a","article-title":"How cilia beat.","volume":"463","author":"TJ Mitchison","year":"2010","journal-title":"Nature"}],"container-title":["PLoS Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pgen.1001255","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T06:33:41Z","timestamp":1637044421000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1001255"}},"subtitle":[],"editor":[{"given":"Jianzhi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2010,12,23]]},"references-count":64,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2010,12,23]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pgen.1001255","relation":{},"ISSN":["1553-7404"],"issn-type":[{"value":"1553-7404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,23]]}}}