{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T11:09:43Z","timestamp":1793444983142,"version":"4.5.0"},"reference-count":73,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2015,2,18]],"date-time":"2015-02-18T00:00:00Z","timestamp":1424217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"DOI":"10.1371\/journal.pgen.1005006","type":"journal-article","created":{"date-parts":[[2015,2,18]],"date-time":"2015-02-18T18:55:24Z","timestamp":1424285724000},"page":"e1005006","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["Regulators of Autophagosome Formation in Drosophila Muscles"],"prefix":"10.1371","volume":"11","author":[{"given":"Jonathan","family":"Zirin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joppe","family":"Nieuwenhuis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Samsonova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Perrimon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,2,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1038\/ncb1007-1102","article-title":"Autophagosome formation: core machinery and adaptations","volume":"9","author":"Z Xie","year":"2007","journal-title":"Nat Cell Biol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.ceb.2009.11.014","article-title":"Mammalian autophagy: core molecular machinery and signaling regulation","volume":"22","author":"Z Yang","year":"2011","journal-title":"Curr Opin Cell Biol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1038\/nrm2708","article-title":"Dynamics and diversity in autophagy mechanisms: lessons from yeast","volume":"10","author":"H Nakatogawa","year":"2009","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1091\/mbc.8.11.2307","article-title":"A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole","volume":"8","author":"SE Rieder","year":"1997","journal-title":"Mol Biol Cell"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.cell.2012.11.001","article-title":"The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes\/lysosomes","volume":"151","author":"E Itakura","year":"2012","journal-title":"Cell"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1091\/mbc.E13-08-0449","article-title":"Interaction of the HOPS complex with Syntaxin 17 mediates autophagosome clearance in Drosophila","volume":"25","author":"S Takats","year":"2014","journal-title":"Mol Biol Cell"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1038\/ncb1740","article-title":"Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking","volume":"10","author":"C Liang","year":"2008","journal-title":"Nat Cell Biol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1091\/mbc.E13-08-0447","article-title":"The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17","volume":"25","author":"P Jiang","year":"2014","journal-title":"Mol Biol Cell"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3690","DOI":"10.1091\/mbc.12.11.3690","article-title":"Autophagosome requires specific early Sec proteins for its formation and NSF\/SNARE for vacuolar fusion","volume":"12","author":"N Ishihara","year":"2001","journal-title":"Mol Biol Cell"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.cell.2012.03.017","article-title":"mTOR signaling in growth control and disease","volume":"149","author":"M Laplante","year":"2012","journal-title":"Cell"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1091\/mbc.E08-12-1248","article-title":"Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy","volume":"20","author":"N Hosokawa","year":"2009","journal-title":"Mol Biol Cell"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1242\/jcs.064576","article-title":"The regulation of autophagy\u2014unanswered questions","volume":"124","author":"Y Chen","year":"2011","journal-title":"J Cell Sci"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/j.cub.2010.04.041","article-title":"DAP1, a novel substrate of mTOR, negatively regulates autophagy","volume":"20","author":"I Koren","year":"2010","journal-title":"Curr Biol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"279","DOI":"10.4161\/auto.7.3.14487","article-title":"Selective autophagy mediated by autophagic adapter proteins","volume":"7","author":"T Johansen","year":"2011","journal-title":"Autophagy"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"219625","DOI":"10.1155\/2012\/219625","article-title":"Autophagy: more than a nonselective pathway","volume":"2012","author":"F Reggiori","year":"2012","journal-title":"Int J Cell Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/nature09204","article-title":"Network organization of the human autophagy system","volume":"466","author":"C Behrends","year":"2010","journal-title":"Nature"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1083\/jcb.78.3.856","article-title":"Isolation and partial characterization of Drosophila myoblasts from primary cultures of embryonic cells","volume":"78","author":"SI Bernstein","year":"1978","journal-title":"J Cell Biol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1242\/dev.012849","article-title":"RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance","volume":"135","author":"J Bai","year":"2008","journal-title":"Development"},{"key":"ref19","article-title":"Integrating protein-protein interaction networks with phenotypes reveals signs of interactions","author":"A Vinayagam","year":"2013","journal-title":"Nat Methods In Press"},{"key":"ref20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1001708","article-title":"Role of autophagy in glycogen breakdown and its relevance to chloroquine myopathy","volume":"11","author":"J Zirin","year":"2013","journal-title":"PloS Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/0014-4886(81)90090-X","article-title":"Inhibition of lysosomal function in red and white skeletal muscles by chloroquine","volume":"71","author":"WT Stauber","year":"1981","journal-title":"Exp Neurol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"6030","DOI":"10.1167\/iovs.10-5278","article-title":"Induction of lysosomal dilatation, arrested autophagy, and cell death by chloroquine in cultured ARPE-19 cells","volume":"51","author":"YH Yoon","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1038\/nprot.2009.147","article-title":"Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening","volume":"4","author":"J Bai","year":"2009","journal-title":"Nat Protoc"},{"key":"ref24","article-title":"Primary cell cultures from Drosophila gastrula embryos","author":"N Perrimon","year":"2011","journal-title":"J Vis Exp"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"553","DOI":"10.4161\/auto.4615","article-title":"Cytosolic LC3 ratio as a sensitive index of macroautophagy in isolated rat hepatocytes and H4-II-E cells","volume":"3","author":"MR Karim","year":"2007","journal-title":"Autophagy"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"23","DOI":"10.4161\/auto.1.1.1495","article-title":"The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo\/lysosomes","volume":"1","author":"ET Bampton","year":"2005","journal-title":"Autophagy"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e9806","DOI":"10.1371\/journal.pone.0009806","article-title":"Nucleocytoplasmic distribution and dynamics of the autophagosome marker EGFP-LC3","volume":"5","author":"KR Drake","year":"2010","journal-title":"PLoS One"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s00281-010-0223-y","article-title":"Drosophila as a model system to study autophagy","volume":"32","author":"J Zirin","year":"2010","journal-title":"Semin Immunopathol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1080\/00034983.1997.11813196","article-title":"Clinical pharmacology and malaria","volume":"91","author":"AM Breckenridge","year":"1997","journal-title":"Ann Trop Med Parasitol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1177\/096120339600500313","article-title":"Controlled trial with chloroquine diphosphate in systemic lupus erythematosus","volume":"5","author":"IM Meinao","year":"1996","journal-title":"Lupus"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1097\/BOR.0b013e32834456bf","article-title":"Re-evaluation of antimalarials in treating rheumatic diseases: re-appreciation and insights into new mechanisms of action","volume":"23","author":"SJ Katz","year":"2011","journal-title":"Curr Opin Rheumatol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1038\/emboj.2012.32","article-title":"A lysosome-to-nucleus signalling mechanism senses and regulates the lysosome via mTOR and TFEB","volume":"31","author":"C Settembre","year":"2012","journal-title":"EMBO J"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1177\/1087057107300646","article-title":"The use of strictly standardized mean difference for hit selection in primary RNA interference high-throughput screening experiments","volume":"12","author":"XD Zhang","year":"2007","journal-title":"J Biomol Screen"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/nmeth.1351","article-title":"Statistical methods for analysis of high-throughput RNA interference screens","volume":"6","author":"A Birmingham","year":"2009","journal-title":"Nat Methods"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1016\/j.febslet.2009.12.047","article-title":"Mechanisms of cross-talk between the ubiquitin-proteasome and autophagy-lysosome systems","volume":"584","author":"VI Korolchuk","year":"2010","journal-title":"FEBS Lett"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1083\/jcb.201002035","article-title":"Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis","volume":"190","author":"IP Nezis","year":"2010","journal-title":"J Cell Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1093\/genetics\/151.2.675","article-title":"Genetic analysis of hook, a gene required for endocytic trafficking in drosophila","volume":"151","author":"H Kramer","year":"1999","journal-title":"Genetics"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"5720","DOI":"10.1093\/emboj\/19.21.5720","article-title":"LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing","volume":"19","author":"Y Kabeya","year":"2000","journal-title":"EMBO J"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.nbd.2008.06.003","article-title":"Neuroprotection of rapamycin in lactacystin-induced neurodegeneration via autophagy enhancement","volume":"32","author":"T Pan","year":"2008","journal-title":"Neurobiol Dis"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1038\/nature05853","article-title":"HDAC6 rescues neurodegeneration and provides an essential link between autophagy and the UPS","volume":"447","author":"UB Pandey","year":"2007","journal-title":"Nature"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"8842","DOI":"10.1073\/pnas.90.19.8842","article-title":"Identification of an essential gene, l(3)73Ai, with a dominant temperature-sensitive lethal allele, encoding a Drosophila proteasome subunit","volume":"90","author":"KJ Saville","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"11382","DOI":"10.1073\/pnas.96.20.11382","article-title":"Dominant-negative mutation in the beta2 and beta6 proteasome subunit genes affect alternative cell fate decisions in the Drosophila sense organ lineage","volume":"96","author":"F Schweisguth","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1002\/neu.10298","article-title":"Tyramine and octopamine have opposite effects on the locomotion of Drosophila larvae","volume":"58","author":"S Saraswati","year":"2004","journal-title":"J Neurobiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"E261","DOI":"10.1371\/journal.pbio.0020261","article-title":"The Rab5 effector Rabankyrin-5 regulates and coordinates different endocytic mechanisms","volume":"2","author":"C Schnatwinkel","year":"2004","journal-title":"PLoS Biol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1111\/j.1365-2443.2006.00997.x","article-title":"Screening for target Rabs of TBC (Tre-2\/Bub2\/Cdc16) domain-containing proteins based on their Rab-binding activity","volume":"11","author":"T Itoh","year":"2006","journal-title":"Genes Cells"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"e1000111","DOI":"10.1371\/journal.pgen.1000111","article-title":"Identification of neural outgrowth genes using genome-wide RNAi","volume":"4","author":"KJ Sepp","year":"2008","journal-title":"PLoS Genet"},{"key":"ref47","first-page":"96","article-title":"Progress and problems in muscle glycogenoses","volume":"30","author":"S DiMauro","year":"2011","journal-title":"Acta Myol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1084\/jem.20130252","article-title":"Phosphofructokinase deficiency impairs ATP generation, autophagy, and redox balance in rheumatoid arthritis T cells","volume":"210","author":"Z Yang","year":"2013","journal-title":"J Exp Med"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1146\/annurev-immunol-032712-095956","article-title":"Metabolic regulation of T lymphocytes","volume":"31","author":"NJ MacIver","year":"2013","journal-title":"Annu Rev Immunol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.yjmcc.2013.09.014","article-title":"Myocardial glycophagy\u2014A specific glycogen handling response to metabolic stress is accentuated in the female heart","volume":"65C","author":"ME Reichelt","year":"2013","journal-title":"J Mol Cell Cardiol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1042\/BC20070019","article-title":"Proteasome function is required to maintain muscle cellular architecture","volume":"99","author":"KF Haas","year":"2007","journal-title":"Biol Cell"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"325","DOI":"10.3390\/cells1030325","article-title":"Autophagy in skeletal muscle homeostasis and in muscular dystrophies","volume":"1","author":"P Grumati","year":"2012","journal-title":"Cells"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.cub.2009.11.022","article-title":"Chaperone-assisted selective autophagy is essential for muscle maintenance","volume":"20","author":"V Arndt","year":"2010","journal-title":"Curr Biol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.ceb.2010.04.007","article-title":"Rab GEFs and GAPs","volume":"22","author":"F Barr","year":"2010","journal-title":"Curr Opin Cell Biol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1128\/MCB.06717-11","article-title":"Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers","volume":"32","author":"D Popovic","year":"2012","journal-title":"Mol Cell Biol"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/nrm3364","article-title":"Illuminating the functional and structural repertoire of human TBC\/RABGAPs","volume":"13","author":"MA Frasa","year":"2012","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"4655","DOI":"10.1074\/jbc.272.8.4655","article-title":"Isolation and characterization of a GTPase activating protein specific for the Rab3 subfamily of small G proteins","volume":"272","author":"K Fukui","year":"1997","journal-title":"J Biol Chem"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"24781","DOI":"10.1074\/jbc.273.38.24781","article-title":"Molecular cloning and characterization of the noncatalytic subunit of the Rab3 subfamily-specific GTPase-activating protein","volume":"273","author":"F Nagano","year":"1998","journal-title":"J Biol Chem"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1042\/BST20120169","article-title":"RAB3GAP1, RAB3GAP2 and RAB18: disease genes in Micro and Martsolf syndromes","volume":"40","author":"MT Handley","year":"2012","journal-title":"Biochem Soc Trans"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/j.neuron.2011.01.025","article-title":"Rab3-GAP controls the progression of synaptic homeostasis at a late stage of vesicle release","volume":"69","author":"M Muller","year":"2011","journal-title":"Neuron"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"e83324","DOI":"10.1371\/journal.pone.0083324","article-title":"Rab39a interacts with phosphatidylinositol 3-kinase and negatively regulates autophagy induced by lipopolysaccharide stimulation in macrophages","volume":"8","author":"S Seto","year":"2013","journal-title":"PLoS One"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1006\/dbio.1996.9987","article-title":"Wingless signaling induces nautilus expression in the ventral mesoderm of the Drosophila embryo","volume":"176","author":"G Ranganayakulu","year":"1996","journal-title":"Dev Biol"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1083\/jcb.200712051","article-title":"The class III PI(3)K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila","volume":"181","author":"G Juhasz","year":"2008","journal-title":"J Cell Biol"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"3663","DOI":"10.1242\/jcs.02502","article-title":"Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules","volume":"118","author":"S Pulipparacharuvil","year":"2005","journal-title":"J Cell Sci"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.neuron.2009.11.002","article-title":"Rab3 dynamically controls protein composition at active zones","volume":"64","author":"ER Graf","year":"2009","journal-title":"Neuron"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1534\/genetics.106.066761","article-title":"Thirty-one flavors of Drosophila rab proteins","volume":"176","author":"J Zhang","year":"2007","journal-title":"Genetics"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.jneumeth.2011.08.037","article-title":"Mudher A Increased throughput assays of locomotor dysfunction in Drosophila larvae","volume":"203","author":"C Sinadinos","journal-title":"J Neurosci Methods"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0074-7742(06)75015-2","article-title":"Selected methods for the anatomical study of Drosophila embryonic and larval neuromuscular junctions","volume":"75","author":"V Budnik","year":"2006","journal-title":"Int Rev Neurobiol"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"CA Schneider","year":"2012","journal-title":"Nat Methods"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"2178","DOI":"10.1101\/gr.1224503","article-title":"OrthoMCL: identification of ortholog groups for eukaryotic genomes","volume":"13","author":"L Li","year":"2003","journal-title":"Genome Res"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/nmeth.2733","article-title":"Integrating protein-protein interaction networks with phenotypes reveals signs of interactions","volume":"11","author":"A Vinayagam","year":"2014","journal-title":"Nat Methods"},{"key":"ref72","doi-asserted-by":"crossref","first-page":"e49386","DOI":"10.1371\/journal.pone.0049386","article-title":"GUItars: a GUI tool for analysis of high-throughput RNA interference screening data","volume":"7","author":"AN Goktug","year":"2012","journal-title":"PLoS One"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1177\/1087057111405851","article-title":"Illustration of SSMD, z score, SSMD*, z* score, and t statistic for hit selection in RNAi high-throughput screens","volume":"16","author":"XD Zhang","year":"2011","journal-title":"J Biomol Screen"}],"container-title":["PLOS Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pgen.1005006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T03:44:15Z","timestamp":1651290255000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1005006"}},"subtitle":[],"editor":[{"given":"Eric","family":"Baehrecke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,2,18]]},"references-count":73,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,2,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pgen.1005006","relation":{},"ISSN":["1553-7404"],"issn-type":[{"value":"1553-7404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,18]]}}}