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Vylkova","year":"2017","journal-title":"PLoS pathogens"},{"issue":"10","key":"pgen.1008634.ref064","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.tibs.2004.08.010","article-title":"The eukaryotic plasma membrane as a nutrient-sensing device","volume":"29","author":"I Holsbeeks","year":"2004","journal-title":"Trends in biochemical sciences"},{"key":"pgen.1008634.ref065","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3389\/fphar.2018.00191","article-title":"Multiple Transceptors for Macro- and Micro-Nutrients Control Diverse Cellular Properties Through the PKA Pathway in Yeast: A Paradigm for the Rapidly Expanding World of Eukaryotic Nutrient Transceptors Up to Those in Human Cells","volume":"9","author":"F Steyfkens","year":"2018","journal-title":"Frontiers in pharmacology"},{"issue":"1","key":"pgen.1008634.ref066","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1432-1033.1970.tb00817.x","article-title":"Mutations affecting the repressibility of arginine biosynthetic enzymes in Saccharomyces cerevisiae","volume":"12","author":"J Bechet","year":"1970","journal-title":"European journal of biochemistry \/ FEBS"},{"issue":"6","key":"pgen.1008634.ref067","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1093\/nar\/20.6.1425","article-title":"Improved method for high efficiency transformation of intact yeast cells","volume":"20","author":"D Gietz","year":"1992","journal-title":"Nucleic acids research"},{"issue":"13","key":"pgen.1008634.ref068","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1002\/yea.320101310","article-title":"New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae","volume":"10","author":"A Wach","year":"1994","journal-title":"Yeast"},{"issue":"4","key":"pgen.1008634.ref069","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/0022-2836(80)90055-8","article-title":"A cis-dominant regulatory mutation linked to the argB-argC gene cluster in Saccharomyces cerevisiae","volume":"139","author":"P Jacobs","year":"1980","journal-title":"Journal of molecular biology"},{"issue":"2","key":"pgen.1008634.ref070","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","article-title":"Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa","volume":"166","author":"H Schagger","year":"1987","journal-title":"Analytical biochemistry"},{"issue":"47","key":"pgen.1008634.ref071","doi-asserted-by":"crossref","first-page":"43939","DOI":"10.1074\/jbc.M102944200","article-title":"The Npr1 kinase controls biosynthetic and endocytic sorting of the yeast Gap1 permease","volume":"276","author":"JO De Craene","year":"2001","journal-title":"The Journal of biological chemistry"},{"issue":"4","key":"pgen.1008634.ref072","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1055\/s-2006-923877","article-title":"Role of AMT1;1 in NH4+ acquisition in Arabidopsis thaliana","volume":"8","author":"M Mayer","year":"2006","journal-title":"Plant biology"}],"updated-by":[{"DOI":"10.1371\/journal.pgen.1008634","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T00:00:00Z","timestamp":1582848000000}}],"container-title":["PLOS Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1008634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,15]],"date-time":"2022-10-15T22:26:05Z","timestamp":1665872765000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1008634"}},"subtitle":[],"editor":[{"given":"Joseph","family":"Heitman","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,2,18]]},"references-count":72,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pgen.1008634","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pgen.1008634","asserted-by":"object"}]},"ISSN":["1553-7404"],"issn-type":[{"value":"1553-7404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,18]]}}}