{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:59:42Z","timestamp":1790521182999,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1009198","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:00:00Z","timestamp":1605571200000}}],"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T00:00:00Z","timestamp":1604275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004794","name":"Centre National Recherche Scientifique","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Aix Marseille Universit\u00e9"},{"name":"JPIAMR \u201cCombinatorial\u201d grant"},{"name":"ANR-10-LABX-62-IBEID"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>The level of antibiotic resistance exhibited by bacteria can vary as a function of environmental conditions. Here, we report that phenazine-methosulfate (PMS), a redox-cycling compound (RCC) enhances resistance to fluoroquinolone (FQ) norfloxacin. Genetic analysis showed that <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> adapts to PMS stress by making Fe-S clusters with the SUF machinery instead of the ISC one. Based upon phenotypic analysis of <jats:italic>soxR<\/jats:italic>, <jats:italic>acrA<\/jats:italic>, and <jats:italic>micF<\/jats:italic> mutants, we showed that PMS antagonizes fluoroquinolone toxicity by SoxR-mediated up-regulation of the AcrAB drug efflux pump. Subsequently, we showed that despite the fact that SoxR could receive its cluster from either ISC or SUF, only SUF is able to sustain efficient SoxR maturation under exposure to prolonged PMS period or high PMS concentrations. This study furthers the idea that Fe-S cluster homeostasis acts as a sensor of environmental conditions, and because its broad influence on cell metabolism, modifies the antibiotic resistance profile of <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pgen.1009198","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T18:47:15Z","timestamp":1604342835000},"page":"e1009198","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":27,"title":["Oxidative stress antagonizes fluoroquinolone drug sensitivity via the SoxR-SUF Fe-S cluster homeostatic axis"],"prefix":"10.1371","volume":"16","author":[{"given":"Audrey","family":"Gerstel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1840-2097","authenticated-orcid":true,"given":"Jordi","family":"Zamarre\u00f1o Beas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohann","family":"Duverger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5511-6375","authenticated-orcid":true,"given":"Emmanuelle","family":"Bouveret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3458-2574","authenticated-orcid":true,"given":"Fr\u00e9d\u00e9ric","family":"Barras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9541-8084","authenticated-orcid":true,"given":"B\u00e9atrice","family":"Py","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,11,2]]},"reference":[{"key":"pgen.1009198.ref001","doi-asserted-by":"crossref","first-page":"S122","DOI":"10.1038\/nm1145","article-title":"Antibacterial 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