{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T06:55:00Z","timestamp":1788332100101,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.pgen.1009356","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T00:00:00Z","timestamp":1613606400000}}],"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T00:00:00Z","timestamp":1612483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>We have gained considerable insight into the mechanisms which recognize and repair DNA damage, but how they adapt to extreme environmental challenges remains poorly understood. Cavefish have proven to be fascinating models for exploring the evolution of DNA repair in the complete absence of UV-induced DNA damage and light. We have previously revealed that the Somalian cavefish<jats:italic>Phreatichthys andruzzii<\/jats:italic>, lacks photoreactivation repair via the loss of light, UV and ROS-induced<jats:italic>photolyase<\/jats:italic>gene transcription mediated by D-box enhancer elements. Here, we explore whether other systems repairing UV-induced DNA damage have been similarly affected in this cavefish model. By performing a comparative study using<jats:italic>P<\/jats:italic>.<jats:italic>andruzzii<\/jats:italic>and the surface-dwelling zebrafish, we provide evidence for a conservation of sunlight-regulated Nucleotide Excision Repair (NER). Specifically, the expression of the<jats:italic>ddb2<\/jats:italic>gene which encodes a key NER recognition factor is robustly induced following exposure to light, UV and oxidative stress in both species. As in the case of the<jats:italic>photolyase<\/jats:italic>genes, D-boxes in the<jats:italic>ddb2<\/jats:italic>promoter are sufficient to induce transcription in zebrafish. Interestingly, despite the loss of D-box-regulated photolyase gene expression in<jats:italic>P<\/jats:italic>.<jats:italic>andruzzii<\/jats:italic>, the D-box is required for<jats:italic>ddb2<\/jats:italic>induction by visible light and oxidative stress in cavefish. However, in the cavefish<jats:italic>ddb2<\/jats:italic>gene this D-box-mediated induction requires cooperation with an adjacent, highly conserved E2F element. Furthermore, while in zebrafish UV-induced<jats:italic>ddb2<\/jats:italic>expression results from transcriptional activation accompanied by stabilization of the<jats:italic>ddb2<\/jats:italic>mRNA, in<jats:italic>P<\/jats:italic>.<jats:italic>andruzzii<\/jats:italic>UV induces<jats:italic>ddb2<\/jats:italic>expression exclusively via an increase in mRNA stability. Thus, we reveal plasticity in the transcriptional and post transcriptional mechanisms regulating the repair of sunlight-induced DNA damage under long-term environmental challenges.<\/jats:p>","DOI":"10.1371\/journal.pgen.1009356","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T03:36:34Z","timestamp":1612755394000},"page":"e1009356","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Regulation of ddb2 expression in blind cavefish and zebrafish reveals plasticity in the control of sunlight-induced DNA damage repair"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7431-5089","authenticated-orcid":true,"given":"Haiyu","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Di Mauro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8192-9134","authenticated-orcid":true,"given":"Sebastian","family":"Lungu-Mitea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Geyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Vallone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0252-3107","authenticated-orcid":true,"given":"Cristiano","family":"Bertolucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1794-9891","authenticated-orcid":true,"given":"Nicholas S.","family":"Foulkes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,2,5]]},"reference":[{"key":"pgen.1009356.ref001","first-page":"169","volume-title":"Early Evolution of DNA Repair Mechanisms. 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