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In this study, we investigated the molecular events accompanying seed failure in hybrids of the closely related species pair\n                    <jats:italic>Capsella rubella<\/jats:italic>\n                    and\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>grandiflora<\/jats:italic>\n                    . Mapping of QTL for the underlying cause of hybrid incompatibility in\n                    <jats:italic>Capsella<\/jats:italic>\n                    identified three QTL that were close to pericentromeric regions. We investigated whether there are specific changes in heterochromatin associated with interspecific hybridizations and found a strong reduction of chromatin condensation in the endosperm, connected with a strong loss of CHG and CHH methylation and random loss of a single chromosome. Consistent with reduced DNA methylation in the hybrid endosperm, we found a disproportionate deregulation of genes located close to pericentromeric regions, suggesting that reduced DNA methylation allows access of transcription factors to targets located in heterochromatic regions. Since the identified QTL were also associated with pericentromeric regions, we propose that relaxation of heterochromatin in response to interspecies hybridization exposes and activates loci leading to hybrid seed failure.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1009370","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T13:42:54Z","timestamp":1613137374000},"page":"e1009370","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Hybrid seed incompatibility in Capsella is connected to chromatin condensation defects in the endosperm"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7279-1417","authenticated-orcid":true,"given":"Katarzyna","family":"Dziasek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7285-9437","authenticated-orcid":true,"given":"Lauriane","family":"Simon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6634-8104","authenticated-orcid":true,"given":"Cl\u00e9ment","family":"Lafon-Placette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Laenen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"W\u00e4rdig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8712-9776","authenticated-orcid":true,"given":"Juan","family":"Santos-Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6020-5102","authenticated-orcid":true,"given":"Tanja","family":"Slotte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2619-4857","authenticated-orcid":true,"given":"Claudia","family":"K\u00f6hler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,2,11]]},"reference":[{"key":"pgen.1009370.ref001","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/j.1469-8137.2012.04182.x","article-title":"Endosperm: Food for humankind and fodder for scientific discoveries","volume":"195","author":"J Li","year":"2012","journal-title":"New Phytol"},{"key":"pgen.1009370.ref002","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1046\/j.1365-313X.2002.01491.x","article-title":"Cell cycle progression during endosperm development in Zea mays depends on parental dosage effects","volume":"32","author":"O Leblanc","year":"2002","journal-title":"Plant J"},{"key":"pgen.1009370.ref003","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1093\/genetics\/107.1.103","article-title":"Ploidy barrier to endosperm development in maize","volume":"107","author":"BY Lin","year":"1984","journal-title":"Genetics"},{"key":"pgen.1009370.ref004","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.tplants.2004.08.007","article-title":"More than a yolk: The short life and complex times of the plant endosperm","author":"LM Costa","year":"2004","journal-title":"Trends in Plant Science"},{"key":"pgen.1009370.ref005","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1242\/dev.077057","article-title":"Endosperm cellularization defines an important developmental transition for embryo development","volume":"139","author":"E Hehenberger","year":"2012","journal-title":"Development"},{"key":"pgen.1009370.ref006","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/BF02861548","article-title":"The endosperm in seed development","volume":"132","author":"RA Brink","year":"1947","journal-title":"Bot Rev"},{"key":"pgen.1009370.ref007","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1111\/j.1469-8137.1961.tb06256.x","article-title":"Studies in british primulas. 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