{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T09:52:44Z","timestamp":1790416364018,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1009512","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000}}],"reference-count":103,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T00:00:00Z","timestamp":1618790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-GM107441"],"award-info":[{"award-number":["R01-GM107441"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K02-AG050774"],"award-info":[{"award-number":["K02-AG050774"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    The actin cytoskeleton is a well-known player in most vital cellular processes, but comparably little is understood about how the actin assembly machinery impacts programmed cell death pathways. In the current study, we explored roles for the human Wiskott-Aldrich Syndrome Protein (WASP) family of actin nucleation factors in DNA damage-induced apoptosis. Inactivation of each WASP-family gene revealed that two of them,\n                    <jats:italic>JMY<\/jats:italic>\n                    and\n                    <jats:italic>WHAMM<\/jats:italic>\n                    , are necessary for rapid apoptotic responses. JMY and WHAMM participate in a p53-dependent cell death pathway by enhancing mitochondrial permeabilization, initiator caspase cleavage, and executioner caspase activation. JMY-mediated apoptosis requires actin nucleation via the Arp2\/3 complex, and actin filaments are assembled in cytoplasmic territories containing clusters of cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    and active caspase-3. The loss of JMY additionally results in significant changes in gene expression, including upregulation of the WHAMM-interacting G-protein RhoD. Depletion or deletion of\n                    <jats:italic>RHOD<\/jats:italic>\n                    increases cell death, suggesting that RhoD normally contributes to cell survival. These results give rise to a model in which JMY and WHAMM promote intrinsic cell death responses that can be opposed by RhoD.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1009512","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T16:44:42Z","timestamp":1618850682000},"page":"e1009512","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["The actin nucleation factors JMY and WHAMM enable a rapid Arp2\/3 complex-mediated intrinsic pathway of apoptosis"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9493-5426","authenticated-orcid":true,"given":"Virginia L.","family":"King","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0401-2549","authenticated-orcid":true,"given":"Nathan K.","family":"Leclair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6147-4408","authenticated-orcid":true,"given":"Alyssa M.","family":"Coulter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3178-9804","authenticated-orcid":true,"given":"Kenneth G.","family":"Campellone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,4,19]]},"reference":[{"issue":"4","key":"pgen.1009512.ref001","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/bjc.1972.33","article-title":"Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics","volume":"26","author":"JF Kerr","year":"1972","journal-title":"Br J 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