{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T13:12:16Z","timestamp":1784121136509,"version":"3.55.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1009728","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T00:00:00Z","timestamp":1630022400000}}],"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T00:00:00Z","timestamp":1629158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R35GM133780"],"award-info":[{"award-number":["R35GM133780"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Robertson Foundation"},{"DOI":"10.13039\/100011331","name":"Monique Weill-Caulier Trust","doi-asserted-by":"publisher","award":["Monique Weill-Caulier Career Scientist Award"],"award-info":[{"award-number":["Monique Weill-Caulier Career Scientist Award"]}],"id":[{"id":"10.13039\/100011331","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000879","name":"Alfred P. Sloan Foundation","doi-asserted-by":"publisher","award":["Alfred P. Sloan Research Fellowship (FG-2018-10627)"],"award-info":[{"award-number":["Alfred P. Sloan Research Fellowship (FG-2018-10627)"]}],"id":[{"id":"10.13039\/100000879","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001447","name":"Rita Allen Foundation","doi-asserted-by":"publisher","award":["Rita Allen Foundation Scholar Program"],"award-info":[{"award-number":["Rita Allen Foundation Scholar Program"]}],"id":[{"id":"10.13039\/100001447","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007204","name":"Vallee Foundation","doi-asserted-by":"publisher","award":["Vallee Scholar Program (VS-2020-35)"],"award-info":[{"award-number":["Vallee Scholar Program (VS-2020-35)"]}],"id":[{"id":"10.13039\/100007204","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","award":["PJT-165884"],"award-info":[{"award-number":["PJT-165884"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Dosage compensation equalizes X-linked expression between XY males and XX females. In male fruit flies, expression levels of the X-chromosome are increased approximately two-fold to compensate for their single X chromosome. In testis, dosage compensation is thought to cease during meiosis; however, the timing and degree of the resulting transcriptional suppression is difficult to separate from global meiotic downregulation of each chromosome. To address this, we analyzed testis single-cell RNA-sequencing (scRNA-seq) data from two\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    strains. We found evidence that the X chromosome is equally transcriptionally active as autosomes in somatic and pre-meiotic cells, and less transcriptionally active than autosomes in meiotic and post-meiotic cells. In cells experiencing dosage compensation, close proximity to MSL (male-specific lethal) chromatin entry sites (CES) correlates with increased X chromosome transcription. We found low or undetectable levels of germline expression of most\n                    <jats:italic>msl<\/jats:italic>\n                    genes,\n                    <jats:italic>mle<\/jats:italic>\n                    ,\n                    <jats:italic>roX1<\/jats:italic>\n                    and\n                    <jats:italic>roX2<\/jats:italic>\n                    via scRNA-seq and RNA-FISH, and no evidence of germline nuclear\n                    <jats:italic>roX1\/2<\/jats:italic>\n                    localization. Our results suggest that, although dosage compensation occurs in somatic and pre-meiotic germ cells in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    testis, there might be non-canonical factors involved in the dosage compensation mechanism. The single-cell expression patterns and enrichment statistics of detected genes can be explored interactively in our database:\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/zhao.labapps.rockefeller.edu\/gene-expr\/\" xlink:type=\"simple\">https:\/\/zhao.labapps.rockefeller.edu\/gene-expr\/<\/jats:ext-link>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1009728","type":"journal-article","created":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T14:33:37Z","timestamp":1629210817000},"page":"e1009728","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":46,"title":["Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2973-6946","authenticated-orcid":true,"given":"Evan","family":"Witt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9187-1703","authenticated-orcid":true,"given":"Zhantao","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6182-7074","authenticated-orcid":true,"given":"Henry M.","family":"Krause","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6776-1996","authenticated-orcid":true,"given":"Li","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,8,17]]},"reference":[{"key":"pgen.1009728.ref001","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1186\/gb-2010-11-8-216","article-title":"Dosage compensation and the global re-balancing of aneuploid genomes","volume":"11","author":"M Prestel","year":"2010","journal-title":"Genome Biol"},{"key":"pgen.1009728.ref002","first-page":"165","article-title":"Evidence of the precision of genetic adaptation","volume":"XLIII","author":"H Muller","year":"1950","journal-title":"Harvey Lecture 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