{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:57:40Z","timestamp":1790776660746,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1009748","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000}}],"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T00:00:00Z","timestamp":1634688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","award":["CNRS-UMR 3525"],"award-info":[{"award-number":["CNRS-UMR 3525"]}],"id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la recherche m\u00e9dicale","doi-asserted-by":"crossref","award":["DBF20160635736"],"award-info":[{"award-number":["DBF20160635736"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Agence nationale de la recherche","award":["ANR-17-CE13-0010-01 UNIBAC"],"award-info":[{"award-number":["ANR-17-CE13-0010-01 UNIBAC"]}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["PTR 245-19"],"award-info":[{"award-number":["PTR 245-19"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Pasteur - Paris University (PPU) International PhD Program, which has received funding from the European Union's Horizon 2020 research and innovation programme","award":["665807"],"award-info":[{"award-number":["665807"]}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Antibiotic resistance has become a major global issue. Understanding the molecular mechanisms underlying microbial adaptation to antibiotics is of keen importance to fight Antimicrobial Resistance (AMR). Aminoglycosides are a class of antibiotics that target the small subunit of the bacterial ribosome, disrupting translational fidelity and increasing the levels of misfolded proteins in the cell. In this work, we investigated the role of VchM, a DNA methyltransferase, in the response of the human pathogen\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    to aminoglycosides. VchM is a\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    specific orphan m5C DNA methyltransferase that generates cytosine methylation at 5\u2019-R\n                    <jats:underline>\n                      <jats:bold>C<\/jats:bold>\n                    <\/jats:underline>\n                    CGGY-3\u2019 motifs. We show that deletion of\n                    <jats:italic>vchM<\/jats:italic>\n                    , although causing a growth defect in absence of stress, allows\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    cells to cope with aminoglycoside stress at both sub-lethal and lethal concentrations of these antibiotics. Through transcriptomic and genetic approaches, we show that\n                    <jats:italic>groESL-2<\/jats:italic>\n                    (a specific set of chaperonin-encoding genes located on the second chromosome of\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    ), are upregulated in cells lacking\n                    <jats:italic>vchM<\/jats:italic>\n                    and are needed for the tolerance of\n                    <jats:italic>vchM<\/jats:italic>\n                    mutant to lethal aminoglycoside treatment, likely by fighting aminoglycoside-induced misfolded proteins. Interestingly, preventing VchM methylation of the four RCCGGY sites located in\n                    <jats:italic>groESL-2<\/jats:italic>\n                    region, leads to a higher expression of these genes in WT cells, showing that the expression of these chaperonins is modulated in\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    by DNA methylation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1009748","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T23:55:24Z","timestamp":1634774124000},"page":"e1009748","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Deficiency in cytosine DNA methylation leads to high chaperonin expression and tolerance to aminoglycosides in Vibrio cholerae"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0098-5140","authenticated-orcid":true,"given":"Andr\u00e9","family":"Carvalho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6482-6002","authenticated-orcid":true,"given":"Didier","family":"Mazel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3477-2685","authenticated-orcid":true,"given":"Zeynep","family":"Baharoglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,10,20]]},"reference":[{"key":"pgen.1009748.ref001","article-title":"Detection of antimicrobial activity in urine for epidemiologic studies of antibiotic use","author":"YC Liu","year":"1999","journal-title":"J Clin 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