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However, little is known about what genes or proteins connecting somatic and germ cells are required for this regulation. Our results show that deficiency for adhesion molecule IGSF11, which is expressed in both Sertoli cells and germ cells, leads to male infertility in mice. Combining a new meiotic fluorescent reporter system with testicular cell transplantation, we demonstrated that IGSF11 is required in both somatic cells and spermatogenic cells for primary spermatocyte development. In the absence of IGSF11, spermatocytes proceed through pachytene, but the pericentric heterochromatin of nonhomologous chromosomes remains inappropriately clustered from late pachytene onward, resulting in undissolved interchromosomal interactions. Hi-C analysis reveals elevated levels of interchromosomal interactions occurring mostly at the chromosome ends. Collectively, our data elucidates that IGSF11 in somatic cells and germ cells is required for pericentric heterochromatin dissociation during diplotene in mouse primary spermatocytes.<\/jats:p>","DOI":"10.1371\/journal.pgen.1009778","type":"journal-article","created":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T18:02:13Z","timestamp":1631037733000},"page":"e1009778","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2259-5335","authenticated-orcid":true,"given":"Bo","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengzhen","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9070-8496","authenticated-orcid":true,"given":"An","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2108-3207","authenticated-orcid":true,"given":"Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3597-3350","authenticated-orcid":true,"given":"Lin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7731-2147","authenticated-orcid":true,"given":"Xuerui","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6926-7203","authenticated-orcid":true,"given":"Kehkooi","family":"Kee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,9,7]]},"reference":[{"issue":"7","key":"pgen.1009778.ref001","doi-asserted-by":"crossref","first-page":"e1003629","DOI":"10.1371\/journal.pgen.1003629","article-title":"Gata4 is required for formation of the genital ridge in mice","volume":"9","author":"YC Hu","year":"2013","journal-title":"PLoS Genet"},{"issue":"7628","key":"pgen.1009778.ref002","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/nature20104","article-title":"Reconstitution in vitro of the entire cycle of the mouse female germ line","volume":"539","author":"O Hikabe","year":"2016","journal-title":"Nature"},{"issue":"3","key":"pgen.1009778.ref003","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.stem.2016.01.017","article-title":"Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro","volume":"18","author":"Q Zhou","year":"2016","journal-title":"Cell Stem Cell"},{"issue":"5","key":"pgen.1009778.ref004","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/S0006-291X(02)02025-9","article-title":"Molecular cloning of a novel immunoglobulin superfamily gene preferentially expressed by brain and testis","volume":"296","author":"S Suzu","year":"2002","journal-title":"Biochem Biophys Res Commun"},{"key":"pgen.1009778.ref005","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/978-1-4614-8090-7_2","article-title":"The IgCAMs CAR, BT-IgSF, and CLMP: structure, function, and diseases","volume":"8","author":"J Schreiber","year":"2014","journal-title":"Adv Neurobiol."},{"issue":"3","key":"pgen.1009778.ref006","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1002\/jcp.20361","article-title":"BT-IgSF, a novel immunoglobulin superfamily protein, functions as a cell adhesion molecule","volume":"204","author":"H Harada","year":"2005","journal-title":"J Cell Physiol"},{"issue":"1","key":"pgen.1009778.ref007","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/nn.4176","article-title":"Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity","volume":"19","author":"S Jang","year":"2016","journal-title":"Nat Neurosci"},{"issue":"52","key":"pgen.1009778.ref008","doi-asserted-by":"crossref","first-page":"21490","DOI":"10.1074\/jbc.RA117.000113","article-title":"The cell adhesion molecule BT-IgSF is essential for a functional blood-testis barrier and male fertility in mice","volume":"292","author":"L Pelz","year":"2017","journal-title":"J Biol Chem"},{"issue":"2","key":"pgen.1009778.ref009","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1159\/000460811","article-title":"Nuclear Architecture of Mouse Spermatocytes: Chromosome Topology, Heterochromatin, and Nucleolus","volume":"151","author":"S. 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