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For this reason, there is significant interest in identifying and characterizing the scaffolds required for receptor localization at different synapses. Here we analyze the role of two\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>elegans<\/jats:italic>\n                    post-synaptic scaffolding proteins (LIN-2\/CASK and FRM-3\/FARP) at cholinergic neuromuscular junctions. Constitutive knockouts or muscle specific inactivation of\n                    <jats:italic>lin-2<\/jats:italic>\n                    and\n                    <jats:italic>frm-3<\/jats:italic>\n                    dramatically reduced spontaneous and evoked post-synaptic currents. These synaptic defects resulted from the decreased abundance of two classes of post-synaptic ionotropic acetylcholine receptors (ACR-16\/CHRNA7 and levamisole-activated AChRs). LIN-2\u2019s AChR scaffolding function is mediated by its SH3 and PDZ domains, which interact with AChRs and FRM-3\/FARP, respectively. Thus, our findings show that post-synaptic LIN-2\/FRM-3 complexes promote cholinergic synaptic transmission by recruiting AChRs to post-synaptic elements.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1010211","type":"journal-article","created":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T13:27:25Z","timestamp":1666618045000},"page":"e1010211","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["CASK and FARP localize two classes of post-synaptic ACh receptors thereby promoting cholinergic transmission"],"prefix":"10.1371","volume":"18","author":[{"given":"Lei","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haowen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7791-3770","authenticated-orcid":true,"given":"Kang-Ying","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Nurrish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5705-2782","authenticated-orcid":true,"given":"Xian-Ting","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan-Xin","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiafan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7418-7179","authenticated-orcid":true,"given":"Joshua 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