{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T02:01:21Z","timestamp":1793325681607,"version":"4.5.0"},"reference-count":73,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T00:00:00Z","timestamp":1670457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000154","name":"Division of Integrative Organismal Systems","doi-asserted-by":"publisher","award":["IOS1557806"],"award-info":[{"award-number":["IOS1557806"]}],"id":[{"id":"10.13039\/100000154","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Missouri Life Sciences Fellowship"},{"name":"University of Missouri Life Sciences Fellowship"},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T34 GM136493"],"award-info":[{"award-number":["T34 GM136493"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    A core component of nearly all bacteria, the cell wall is an ideal target for broad spectrum antibiotics. Many bacteria have evolved strategies to sense and respond to antibiotics targeting cell wall synthesis, especially in the soil where antibiotic-producing bacteria compete with one another. Here we show that cell wall stress caused by both chemical and genetic inhibition of the essential, bifunctional penicillin-binding protein PBP1a prevents microcolony formation and activates the canonical host-invasion two-component system ChvG-ChvI in\n                    <jats:italic>Agrobacterium tumefaciens<\/jats:italic>\n                    . Using RNA-seq, we show that depletion of PBP1a for 6 hours results in a downregulation in transcription of flagellum-dependent motility genes and an upregulation in transcription of type VI secretion and succinoglycan biosynthesis genes, a hallmark of the ChvG-ChvI regulon. Depletion of PBP1a for 16 hours, results in differential expression of many additional genes and may promote a stress response, resembling those of sigma factors in other bacteria. Remarkably, the overproduction of succinoglycan causes cell spreading and deletion of the succinoglycan biosynthesis gene\n                    <jats:italic>exoA<\/jats:italic>\n                    restores microcolony formation. Treatment with cefsulodin phenocopies depletion of PBP1a and we correspondingly find that\n                    <jats:italic>chvG<\/jats:italic>\n                    and\n                    <jats:italic>chvI<\/jats:italic>\n                    mutants are hypersensitive to cefsulodin. This hypersensitivity only occurs in response to treatment with \u03b2-lactam antibiotics, suggesting that the ChvG-ChvI pathway may play a key role in resistance to antibiotics targeting cell wall synthesis. Finally, we provide evidence that ChvG-ChvI likely has a conserved role in conferring resistance to cell wall stress within the Alphaproteobacteria that is independent of the ChvG-ChvI repressor ExoR.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1010274","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T13:24:35Z","timestamp":1670505875000},"page":"e1010274","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Activation of ChvG-ChvI regulon by cell wall stress confers resistance to \u03b2-lactam antibiotics and initiates surface spreading in Agrobacterium tumefaciens"],"prefix":"10.1371","volume":"18","author":[{"given":"Michelle A.","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob M.","family":"Bouchier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amara K.","family":"Mason","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7558-7155","authenticated-orcid":true,"given":"Pamela J. 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