{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T18:16:26Z","timestamp":1784225786704,"version":"3.55.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1010358","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000}}],"reference-count":74,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2022,9,9]],"date-time":"2022-09-09T00:00:00Z","timestamp":1662681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R15GM119117-01"],"award-info":[{"award-number":["R15GM119117-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009496","name":"Oklahoma Agricultural Experiment Station","doi-asserted-by":"publisher","award":["OKL02961"],"award-info":[{"award-number":["OKL02961"]}],"id":[{"id":"10.13039\/100009496","id-type":"DOI","asserted-by":"publisher"}]},{"name":"USDA National Institute of Food and Agriculture -Hatch Project","award":["9377"],"award-info":[{"award-number":["9377"]}]},{"name":"CASNR\/OAES Scholarship"},{"name":"Niblack Research Scholarship"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Stu2 in\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>cerevisiae<\/jats:italic>\n                    is a member of the XMAP215\/Dis1\/CKAP5\/ch-TOG family of MAPs and has multiple functions in controlling microtubules, including microtubule polymerization, microtubule depolymerization, linking chromosomes to the kinetochore, and assembly of \u03b3-TuSCs at the SPB. Whereas phosphorylation has been shown to be critical for Stu2 localization at the kinetochore, other regulatory mechanisms that control Stu2 function are still poorly understood. Here, we show that a novel form of Stu2 regulation occurs through the acetylation of three lysine residues at K252, K469, and K870, which are located in three distinct domains of Stu2. Alteration of acetylation through acetyl-mimetic and acetyl-blocking mutations did not impact the essential function of Stu2. Instead, these mutations lead to a decrease in chromosome stability, as well as changes in resistance to the microtubule depolymerization drug, benomyl. In agreement with our\n                    <jats:italic>in silico<\/jats:italic>\n                    modeling, several acetylation-mimetic mutants displayed increased interactions with \u03b3-tubulin. Taken together, these data suggest that Stu2 acetylation can govern multiple Stu2 functions, including chromosome stability and interactions at the SPB.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1010358","type":"journal-article","created":{"date-parts":[[2022,9,9]],"date-time":"2022-09-09T17:03:54Z","timestamp":1662743034000},"page":"e1010358","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["The TOG protein Stu2 is regulated by acetylation"],"prefix":"10.1371","volume":"18","author":[{"given":"Matt A.","family":"Greenlee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1039-2936","authenticated-orcid":true,"given":"Braden","family":"Witt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3213-9659","authenticated-orcid":true,"given":"Jeremy A.","family":"Sabo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7031-4249","authenticated-orcid":true,"given":"Savannah C.","family":"Morris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7052-7567","authenticated-orcid":true,"given":"Rita 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