{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:21:40Z","timestamp":1788826900942,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.pgen.1010737","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T00:00:00Z","timestamp":1683504000000}}],"reference-count":129,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T00:00:00Z","timestamp":1682467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000294","name":"Medical Research Scotland","doi-asserted-by":"crossref","award":["422 FRG"],"award-info":[{"award-number":["422 FRG"]}],"id":[{"id":"10.13039\/501100000294","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Royal Academy of Engineering Research Chair Scheme","award":["RCSRF2021\\11\\15"],"award-info":[{"award-number":["RCSRF2021\\11\\15"]}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>Diphtheria is a respiratory disease caused by <jats:italic>Corynebacterium diphtheriae<\/jats:italic>. While the toxin-based vaccine has helped control outbreaks of the disease since the mid-20<jats:sup>th<\/jats:sup> century there has been an increase in cases in recent years, including systemic infections caused by non-toxigenic <jats:italic>C<\/jats:italic>. <jats:italic>diphtheriae<\/jats:italic> strains. Here we describe the first study of gene essentiality in <jats:italic>C<\/jats:italic>. <jats:italic>diphtheriae<\/jats:italic>, providing the most-dense <jats:underline>Tra<\/jats:underline>nsposon <jats:underline>D<\/jats:underline>irected <jats:underline>I<\/jats:underline>nsertion <jats:underline>S<\/jats:underline>equencing (TraDIS) library in the phylum Actinobacteriota. This high-density library has allowed the identification of conserved genes across the genus and phylum with essential function and enabled the elucidation of essential domains within the resulting proteins including those involved in cell envelope biogenesis. Validation of these data through protein mass spectrometry identified hypothetical and uncharacterized proteins in the proteome which are also represented in the vaccine. These data are an important benchmark and useful resource for the <jats:italic>Corynebacterium<\/jats:italic>, <jats:italic>Mycobacterium<\/jats:italic>, <jats:italic>Nocardia<\/jats:italic> and <jats:italic>Rhodococcus<\/jats:italic> research community. It enables the identification of novel antimicrobial and vaccine targets and provides a basis for future studies of Actinobacterial biology.<\/jats:p>","DOI":"10.1371\/journal.pgen.1010737","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T17:51:34Z","timestamp":1682531494000},"page":"e1010737","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["A multiomic approach to defining the essential genome of the globally important pathogen Corynebacterium diphtheriae"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4846-6566","authenticated-orcid":true,"given":"Emily C. 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