{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T16:08:14Z","timestamp":1788624494312,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.pgen.1010751","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T00:00:00Z","timestamp":1684195200000}}],"reference-count":61,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T00:00:00Z","timestamp":1683158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS 2151032"],"award-info":[{"award-number":["IOS 2151032"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS 2151032"],"award-info":[{"award-number":["IOS 2151032"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS 1732253"],"award-info":[{"award-number":["IOS 1732253"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"John Ingle Innovation"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Methyl salicylate is an important inter- and intra-plant signaling molecule, but is deemed undesirable by humans when it accumulates to high levels in ripe fruits. Balancing the tradeoff between consumer satisfaction and overall plant health is challenging as the mechanisms regulating volatile levels have not yet been fully elucidated. In this study, we investigated the accumulation of methyl salicylate in ripe fruits of tomatoes that belong to the red-fruited clade. We determine the genetic diversity and the interaction of four known loci controlling methyl salicylate levels in ripe fruits. In addition to\n                    <jats:italic>Non-Smoky Glucosyl Transferase 1<\/jats:italic>\n                    (\n                    <jats:italic>NSGT1<\/jats:italic>\n                    ), we uncovered extensive genome structural variation (SV) at the\n                    <jats:italic>Methylesterase<\/jats:italic>\n                    (\n                    <jats:italic>MES)<\/jats:italic>\n                    locus. This locus contains four tandemly duplicated\n                    <jats:italic>Methylesterase<\/jats:italic>\n                    genes and genome sequence investigations at the locus identified nine distinct haplotypes. Based on gene expression and results from biparental crosses, functional and non-functional haplotypes for\n                    <jats:italic>MES<\/jats:italic>\n                    were identified. The combination of the non-functional\n                    <jats:italic>MES<\/jats:italic>\n                    haplotype 2 and the non-functional\n                    <jats:italic>NSGT1<\/jats:italic>\n                    haplotype IV or V in a GWAS panel showed high methyl salicylate levels in ripe fruits, particularly in accessions from Ecuador, demonstrating a strong interaction between these two loci and suggesting an ecological advantage. The genetic variation at the other two known loci,\n                    <jats:italic>Salicylic Acid Methyl Transferase 1<\/jats:italic>\n                    (\n                    <jats:italic>SAMT1<\/jats:italic>\n                    ) and tomato\n                    <jats:italic>UDP Glycosyl Transferase 5<\/jats:italic>\n                    (\n                    <jats:italic>SlUGT5<\/jats:italic>\n                    ), did not explain volatile variation in the red-fruited tomato germplasm, suggesting a minor role in methyl salicylate production in red-fruited tomato. Lastly, we found that most heirloom and modern tomato accessions carried a functional\n                    <jats:italic>MES<\/jats:italic>\n                    and a non-functional\n                    <jats:italic>NSGT1<\/jats:italic>\n                    haplotype, ensuring acceptable levels of methyl salicylate in fruits. Yet, future selection of the functional\n                    <jats:italic>NSGT1<\/jats:italic>\n                    allele could potentially improve flavor in the modern germplasm.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1010751","type":"journal-article","created":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T14:08:43Z","timestamp":1683209323000},"page":"e1010751","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Structural variation underlies functional diversity at methyl salicylate loci in tomato"],"prefix":"10.1371","volume":"19","author":[{"given":"Manoj","family":"Sapkota","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5184-8587","authenticated-orcid":true,"given":"Lara","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2186-9285","authenticated-orcid":true,"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasin","family":"Topcu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Tieman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4963-7427","authenticated-orcid":true,"given":"Esther","family":"van der Knaap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,5,4]]},"reference":[{"key":"pgen.1010751.ref001","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1146\/annurev.ecolsys.38.091206.095601","article-title":"Wake up and smell the roses: the ecology and evolution of floral scent","author":"RA Raguso","year":"2008","journal-title":"Annual review of ecology, evolution, and systematics."},{"issue":"2","key":"pgen.1010751.ref002","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1111\/nph.13243","article-title":"Ecology and evolution of floral volatile-mediated information transfer in plants","volume":"206","author":"FP Schiestl","year":"2015","journal-title":"New Phytologist"},{"issue":"3","key":"pgen.1010751.ref003","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S1369-5266(02)00251-0","article-title":"The formation and function of plant volatiles: perfumes for pollinator attraction and defense","volume":"5","author":"E Pichersky","year":"2002","journal-title":"Current opinion in plant biology"},{"issue":"6","key":"pgen.1010751.ref004","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.actao.2011.06.003","article-title":"When should fig fruit produce volatiles? 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