{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:48:39Z","timestamp":1767181719165,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.pgen.1010805","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:00:00Z","timestamp":1688515200000}}],"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T00:00:00Z","timestamp":1687392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018835","name":"Albert-Heim-Stiftung","doi-asserted-by":"publisher","award":["138"],"award-info":[{"award-number":["138"]}],"id":[{"id":"10.13039\/100018835","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Pelger-Hu\u00ebt anomaly (PHA) in humans is an autosomal dominant hematological phenotype without major clinical consequences. PHA involves a characteristic hyposegmentation of granulocytes (HG). Human PHA is caused by heterozygous loss of function variants in the\n                    <jats:italic>LBR<\/jats:italic>\n                    gene encoding lamin receptor B. Bi-allelic variants and complete deficiency of\n                    <jats:italic>LBR<\/jats:italic>\n                    cause the much more severe Greenberg skeletal dysplasia which is lethal in utero and characterized by massive skeletal malformation and gross fetal hydrops. HG phenotypes have also been described in domestic animals and homology to human PHA has been claimed in the literature. We studied a litter of Australian Shepherd Dogs with four stillborn puppies in which both parents had an HG phenotype. Linkage analysis excluded\n                    <jats:italic>LBR<\/jats:italic>\n                    as responsible gene for the stillborn puppies. We then investigated the HG phenotype in Australian Shepherd Dogs independently of the prenatal lethality. Genome-wide association mapped the HG locus to chromosome 27 and established an autosomal recessive mode of inheritance. Whole genome sequencing identified a splice site variant in\n                    <jats:italic>LMBR1L<\/jats:italic>\n                    , c.191+1G&gt;A, as most likely causal variant for the HG phenotype. The mutant allele abrogates the expression of the longer X2 isoform but does not affect transcripts encoding the shorter X1 isoform of the LMBR1L protein. The homozygous mutant\n                    <jats:italic>LMBR1L<\/jats:italic>\n                    genotype associated with HG is common in Australian Shepherd Dogs and was found in 39 of 300 genotyped dogs (13%). Our results point to a previously unsuspected function of\n                    <jats:italic>LMBR1L<\/jats:italic>\n                    in the myeloid lineage of leukocytes.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1010805","type":"journal-article","created":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T13:34:44Z","timestamp":1687527284000},"page":"e1010805","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Autosomal recessive hyposegmentation of granulocytes in Australian Shepherd Dogs indicates a role for LMBR1L in myeloid leukocytes"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6666-0130","authenticated-orcid":true,"given":"Bianca","family":"Lourdes Frehner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3275-5929","authenticated-orcid":true,"given":"Matthias","family":"Christen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris M.","family":"Reichler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vidhya","family":"Jagannathan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilisa","family":"Novacco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Riond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laureen M.","family":"Peters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Su\u00e1rez S\u00e1nchez-Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldona","family":"Pie\u0144kowska-Schelling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Schelling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Kipar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0553-4880","authenticated-orcid":true,"given":"Tosso","family":"Leeb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0939-2859","authenticated-orcid":true,"given":"Orsolya","family":"Balogh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,6,22]]},"reference":[{"key":"pgen.1010805.ref001","first-page":"1178","article-title":"Demonstratie van een paar zeldzaam voorkomende typen van bloedlichaampjes en bespreking der pati\u00ebnten","volume":"72","author":"K. 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Undritz","year":"1939","journal-title":"Schweiz Med Wochenschr"},{"key":"pgen.1010805.ref012","first-page":"551","article-title":"Die Pelger-Anomalie der Leukocyten und die pathologische Anatomie des neugeborenen homozygoten Pelger-Kaninchens","volume":"29","author":"H. Klein","year":"1949","journal-title":"Z Mensch Vererb Konstitutionsl"},{"key":"pgen.1010805.ref013","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1093\/oxfordjournals.jhered.a106108","article-title":"The Pelger-anomaly in man and rabbit; a mendelian character of the nuclei of the leucocytes","volume":"41","author":"H. 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