{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T01:54:28Z","timestamp":1783821268208,"version":"3.55.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1010899","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000}}],"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270846"],"award-info":[{"award-number":["32270846"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01-AR081081"],"award-info":[{"award-number":["R01-AR081081"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01-DK117981"],"award-info":[{"award-number":["R01-DK117981"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>Tissues are subject to multiple mechanical inputs at the cellular level that influence their overall shape and function. In the small intestine, actomyosin contractility can be induced by many physiological and pathological inputs. However, we have little understanding of how contractility impacts the intestinal epithelium on a cellular and tissue level. In this study, we probed the cell and tissue-level effects of contractility by using mouse models to genetically increase the level of myosin activity in the two distinct morphologic compartments of the intestinal epithelium, the crypts and villi. We found that increased contractility in the villar compartment caused shape changes in the cells that expressed the transgene and their immediate neighbors. While there were no discernable effects on villar architecture or cell polarity, even low levels of transgene induction in the villi caused non-cell autonomous hyperproliferation of the transit amplifying cells in the crypt, driving increased cell flux through the crypt-villar axis. In contrast, induction of increased contractility in the proliferating cells of the crypts resulted in nuclear deformations, DNA damage, and apoptosis. This study reveals the complex and diverse responses of different intestinal epithelial cells to contractility and provides important insight into mechanical regulation of intestinal physiology.<\/jats:p>","DOI":"10.1371\/journal.pgen.1010899","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T13:40:39Z","timestamp":1711114839000},"page":"e1010899","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Compartment specific responses to contractility in the small intestinal epithelium"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8912-6851","authenticated-orcid":true,"given":"Taylor","family":"Hinnant","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxiu","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3901-7013","authenticated-orcid":true,"given":"Terry","family":"Lechler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,3,22]]},"reference":[{"issue":"2","key":"pgen.1010899.ref001","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.devcel.2020.11.025","article-title":"Mechanics of Development.","volume":"56","author":"K Goodwin","year":"2021","journal-title":"Developmental Cell"},{"key":"pgen.1010899.ref002","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.semcdb.2021.06.006","article-title":"Feeling the force: Multiscale force sensing and transduction at the cell-cell interface.","volume":"120","author":"A Inman","year":"2021","journal-title":"Seminars in Cell & Developmental Biology."},{"issue":"1","key":"pgen.1010899.ref003","article-title":"Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets.","volume":"8","author":"X Di","year":"2023","journal-title":"Signal Transduction and Targeted Therapy"},{"issue":"1","key":"pgen.1010899.ref004","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1146\/annurev.cellbio.22.010305.104315","article-title":"Of Extracellular Matrix, Scaffolds, and Signaling: Tissue Architecture Regulates Development, Homeostasis, and Cancer","volume":"22","author":"CM Nelson","year":"2006","journal-title":"Annual Review of Cell and Developmental Biology"},{"issue":"9","key":"pgen.1010899.ref005","doi-asserted-by":"crossref","first-page":"2204594","DOI":"10.1002\/advs.202204594","article-title":"Static and Dynamic: Evolving Biomaterial Mechanical Properties to Control Cellular Mechanotransduction.","volume":"10","author":"W Xie","year":"2023","journal-title":"Advanced Science"},{"issue":"5","key":"pgen.1010899.ref006","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1007\/s12551-022-00997-9","article-title":"Molecular determinants of intrinsic cellular stiffness in health and disease.","volume":"14","author":"Z Su","year":"2022","journal-title":"Biophysical Reviews"},{"issue":"4","key":"pgen.1010899.ref007","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/aja.1001410403","article-title":"Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine I","volume":"141","author":"H Cheng","year":"1974","journal-title":"Columnar cell. 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