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In\n                    <jats:italic>D<\/jats:italic>\n                    .\n                    <jats:italic>melanogaster<\/jats:italic>\n                    , the\n                    <jats:italic>flamenco<\/jats:italic>\n                    locus is a master suppressor of TEs, preventing the mobilization of certain endogenous retrovirus-like TEs from somatic ovarian support cells to the germline. It is transcribed by Pol II as a long (100s of kb), single-stranded, primary transcript, and metabolized into ~24\u201332 nt Piwi-interacting RNAs (piRNAs) that target active TEs via antisense complementarity.\n                    <jats:italic>flamenco<\/jats:italic>\n                    is thought to operate as a trap, owing to its high content of recent horizontally transferred TEs that are enriched in antisense orientation. Using newly-generated long read genome data, which is critical for accurate assembly of repetitive sequences, we find that\n                    <jats:italic>flamenco<\/jats:italic>\n                    has undergone radical transformations in sequence content and even copy number across\n                    <jats:italic>simulans<\/jats:italic>\n                    clade Drosophilid species.\n                    <jats:italic>Drosophila simulans flamenco<\/jats:italic>\n                    has duplicated and diverged, and neither copy exhibits synteny with\n                    <jats:italic>D<\/jats:italic>\n                    .\n                    <jats:italic>melanogaster<\/jats:italic>\n                    beyond the core promoter. Moreover,\n                    <jats:italic>flamenco<\/jats:italic>\n                    organization is highly variable across\n                    <jats:italic>D<\/jats:italic>\n                    .\n                    <jats:italic>simulans<\/jats:italic>\n                    individuals. Next, we find that\n                    <jats:italic>D<\/jats:italic>\n                    .\n                    <jats:italic>simulans<\/jats:italic>\n                    and\n                    <jats:italic>D<\/jats:italic>\n                    .\n                    <jats:italic>mauritiana flamenco<\/jats:italic>\n                    display signatures of a dual-stranded cluster, with ping-pong signals in the testis and\/or embryo. This is accompanied by increased copy numbers of germline TEs, consistent with these regions operating as functional dual-stranded clusters. Overall, the physical and functional diversity of\n                    <jats:italic>flamenco<\/jats:italic>\n                    orthologs is testament to the extremely dynamic consequences of TE arms races on genome organization, not only amongst highly related species, but even amongst individuals.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1010914","type":"journal-article","created":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T13:54:43Z","timestamp":1693317283000},"page":"e1010914","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Rapid evolutionary diversification of the flamenco locus across simulans clade Drosophila species"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2401-0644","authenticated-orcid":true,"given":"Sarah","family":"Signor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Vedanayagam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard Y.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Wierzbicki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Kofler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric 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