{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T10:14:52Z","timestamp":1767176092224,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011151","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000}}],"reference-count":84,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T00:00:00Z","timestamp":1725840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UM-PUHSC Joint Institute","award":["BMU20110177 and BMU20160530"],"award-info":[{"award-number":["BMU20110177 and BMU20160530"]}]},{"name":"National High Level Hospital Clinical Research Funding","award":["2022CR71"],"award-info":[{"award-number":["2022CR71"]}]},{"name":"National Key Research and Development Program of China","award":["2021YFC2500500 and 2021YFC2500503"],"award-info":[{"award-number":["2021YFC2500500 and 2021YFC2500503"]}]},{"name":"Key Laboratory of Molecular Cardiovascular Sciences"},{"name":"National Health Commission Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL139672, R01HL122684, R01HL086694, R35HL161016"],"award-info":[{"award-number":["R01HL139672, R01HL122684, R01HL086694, R35HL161016"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Michigan A. Alfred Taubman Institute"},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR002240"],"award-info":[{"award-number":["UL1TR002240"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Michigan Medical School Office of Research"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Genetic studies of blood pressure (BP) traits to date have been performed on conventional measures by brachial cuff sphygmomanometer for systolic BP (SBP) and diastolic BP, integrating several physiologic occurrences. Genetic associations with central SBP (cSBP) have not been well-studied. Genetic discovery studies of BP have been most often performed in European-ancestry samples. Here, we investigated genetic associations with cSBP in a Chinese population and functionally validated the impact of a novel associated coiled-coil domain containing 93 (\n                    <jats:italic>CCDC93<\/jats:italic>\n                    ) gene on BP regulation. An exome-wide association study (EWAS) was performed using a mixed linear model of non-invasive cSBP and peripheral BP traits in a Han Chinese population (N = 5,954) from Beijing, China genotyped with a customized Illumina ExomeChip array. We identified four SNP-trait associations with three SNPs, including two novel associations (rs2165468-SBP and rs33975708-cSBP). rs33975708 is a coding variant in the\n                    <jats:italic>CCDC93<\/jats:italic>\n                    gene, c.535C&gt;T, p.Arg179Cys (MAF = 0.15%), and was associated with increased cSBP (\u03b2 = 29.3 mmHg,\n                    <jats:italic>P<\/jats:italic>\n                    = 1.23x10\n                    <jats:sup>-7<\/jats:sup>\n                    ). CRISPR\/Cas9 genome editing was used to model the effect of\n                    <jats:italic>Ccdc93<\/jats:italic>\n                    loss in mice. Homozygous\n                    <jats:italic>Ccdc93<\/jats:italic>\n                    deletion was lethal prior to day 10.5 of embryonic development.\n                    <jats:italic>Ccdc93<\/jats:italic>\n                    <jats:sup>+\/-<\/jats:sup>\n                    heterozygous mice were viable and morphologically normal, with 1.3-fold lower aortic Ccdc93 protein expression (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0041) and elevated SBP as compared to littermate\n                    <jats:italic>Ccdc93<\/jats:italic>\n                    <jats:sup>+\/+<\/jats:sup>\n                    controls (110\u00b18 mmHg vs 125\u00b110 mmHg,\n                    <jats:italic>P<\/jats:italic>\n                    = 0.016). Wire myography of\n                    <jats:italic>Ccdc93<\/jats:italic>\n                    <jats:sup>+\/-<\/jats:sup>\n                    aortae showed impaired acetylcholine-induced relaxation and enhanced phenylephrine-induced contraction. RNA-Seq transcriptome analysis of\n                    <jats:italic>Ccdc93<\/jats:italic>\n                    <jats:sup>+\/-<\/jats:sup>\n                    mouse thoracic aortae identified significantly enriched pathways altered in fatty acid metabolism and mitochondrial metabolism. Plasma free fatty acid levels were elevated in\n                    <jats:italic>Ccdc93<\/jats:italic>\n                    <jats:sup>+\/-<\/jats:sup>\n                    mice (96\u00b17mM vs 124\u00b113mM,\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0031) and aortic mitochondrial dysfunction was observed through aberrant Parkin and Nix protein expression. Together, our genetic and functional studies support a novel role of\n                    <jats:italic>CCDC93<\/jats:italic>\n                    in the regulation of BP through its effects on vascular mitochondrial function and endothelial function.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011151","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T13:30:51Z","timestamp":1725888651000},"page":"e1011151","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic variation in CCDC93 is associated with elevated central systolic blood pressure, impaired arterial relaxation, and mitochondrial dysfunction"],"prefix":"10.1371","volume":"20","author":[{"given":"Nitin","family":"Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Lee","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina L.","family":"Hunker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangfang","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinghua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianjia","family":"Ning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jifeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. Eugene","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Huo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3663-5249","authenticated-orcid":true,"given":"Santhi K.","family":"Ganesh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,9,9]]},"reference":[{"issue":"10100","key":"pgen.1011151.ref001","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/S0140-6736(17)32366-8","article-title":"Global, regional, and national comparative risk assessment of 84 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990\u20132016: a systematic analysis for the 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