{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:39:20Z","timestamp":1790145560967,"version":"4.0.1"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011353","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000}}],"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["CRG\/2018\/001988"],"award-info":[{"award-number":["CRG\/2018\/001988"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"name":"DBT-IISC","award":["BT\/PR27952\/IN\/22\/21\/2018"],"award-info":[{"award-number":["BT\/PR27952\/IN\/22\/21\/2018"]}]},{"name":"DST-FIST","award":["SR\/FST\/LSII-045\/2016(G)"],"award-info":[{"award-number":["SR\/FST\/LSII-045\/2016(G)"]}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>Mitochondrial integrity is a crucial determinant of overall cellular health. Mitochondrial dysfunction and impediments in regulating organellar homeostasis contribute majorly to the pathophysiological manifestation of several neurological disorders. Mutations in human DJ-1 (<jats:italic>PARK7<\/jats:italic>) have been implicated in the deregulation of mitochondrial homeostasis, a critical cellular etiology observed in Parkinson\u2019s disease progression. DJ-1 is a multifunctional protein belonging to the DJ-1\/ThiJ\/PfpI superfamily, conserved across the phylogeny. Although the pathophysiological significance of DJ-1 has been well-established, the underlying molecular mechanism(s) by which DJ-1 paralogs modulate mitochondrial maintenance and other cellular processes remains elusive. Using <jats:italic>Saccharomyces cerevisiae<\/jats:italic> as the model organism, we unravel the intricate mechanism by which yeast DJ-1 paralogs (collectively called Hsp31 paralogs) modulate mitochondrial homeostasis. Our study establishes a genetic synthetic interaction between Ubp2, a cysteine-dependent deubiquitinase, and DJ-1 paralogs. In the absence of DJ-1 paralogs, mitochondria adapt to a highly tubular network due to enhanced expression of Fzo1. Intriguingly, the loss of Ubp2 restores the mitochondrial integrity in the DJ-1 deletion background by modulating the ubiquitination status of Fzo1. Besides, the loss of Ubp2 in the absence of DJ-1 restores mitochondrial respiration and functionality by regulating the mitophagic flux. Further, Ubp2 deletion makes cells resistant to oxidative stress without DJ-1 paralogs. For the first time, our study deciphers functional crosstalk between Ubp2 and DJ-1 in regulating mitochondrial homeostasis and cellular health.<\/jats:p>","DOI":"10.1371\/journal.pgen.1011353","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T13:47:24Z","timestamp":1751550444000},"page":"e1011353","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ubp2 modulates DJ-1-mediated redox-dependent mitochondrial dynamics in Saccharomyces 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