{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:13:03Z","timestamp":1791612783490,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011468","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T00:00:00Z","timestamp":1733097600000}}],"reference-count":107,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019180","name":"HORIZON EUROPE European Research Council","doi-asserted-by":"publisher","award":["818696"],"award-info":[{"award-number":["818696"]}],"id":[{"id":"10.13039\/100019180","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["CRSII5_209412"],"award-info":[{"award-number":["CRSII5_209412"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","award":["55008733"],"award-info":[{"award-number":["55008733"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["390939984"],"award-info":[{"award-number":["390939984"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>Inositol pyrophosphates (PP-InsPs) are nutrient messengers whose cellular levels are precisely regulated. Diphosphoinositol pentakisphosphate kinases (PPIP5Ks) generate the active signaling molecule 1,5-InsP<jats:sub>8<\/jats:sub>. PPIP5Ks harbor phosphatase domains that hydrolyze PP-InsPs. Plant and Fungi Atypical Dual Specificity Phosphatases (PFA-DSPs) and NUDIX phosphatases (NUDTs) are also involved in PP-InsP degradation. Here, we analyze the relative contributions of the three different phosphatase families to plant PP-InsP catabolism. We report the biochemical characterization of inositol pyrophosphate phosphatases from Arabidopsis and <jats:italic>Marchantia polymorpha<\/jats:italic>. Overexpression of different PFA-DSP and NUDT enzymes affects PP-InsP levels and leads to stunted growth phenotypes in Arabidopsis. <jats:italic>nudt17\/18\/21<\/jats:italic> knock-out mutants have altered PP-InsP pools and gene expression patterns, but no apparent growth defects. In contrast, <jats:italic>Marchantia polymorpha<\/jats:italic> Mp<jats:italic>pfa-dsp1<\/jats:italic><jats:sup><jats:italic>ge<\/jats:italic><\/jats:sup>, Mp<jats:italic>nudt1<\/jats:italic><jats:sup><jats:italic>ge<\/jats:italic><\/jats:sup> and Mp<jats:italic>vip1<\/jats:italic><jats:sup><jats:italic>ge<\/jats:italic><\/jats:sup> mutants display severe growth and developmental phenotypes and associated changes in cellular PP-InsP levels. Analysis of Mp<jats:italic>pfa-dsp1<\/jats:italic><jats:sup><jats:italic>ge<\/jats:italic><\/jats:sup>and Mp<jats:italic>vip1<\/jats:italic><jats:sup><jats:italic>ge<\/jats:italic><\/jats:sup> mutants supports a role for PP-InsPs in Marchantia phosphate signaling, and additional functions in nitrate homeostasis and cell wall biogenesis. Simultaneous elimination of two phosphatase activities enhanced the observed growth phenotypes. Taken together, PPIP5K, PFA-DSP and NUDT inositol pyrophosphate phosphatases regulate growth and development by collectively shaping plant PP-InsP pools.<\/jats:p>","DOI":"10.1371\/journal.pgen.1011468","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T18:37:47Z","timestamp":1731436667000},"page":"e1011468","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Inositol pyrophosphate catabolism by three families of phosphatases regulates plant growth and development"],"prefix":"10.1371","volume":"20","author":[{"given":"Florian","family":"Laurent","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2215-7901","authenticated-orcid":true,"given":"Simon 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