{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:47:16Z","timestamp":1792788436367,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011591","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000}}],"reference-count":64,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001793","name":"Queensland University of Technology","doi-asserted-by":"publisher","award":["ECRIG2024"],"award-info":[{"award-number":["ECRIG2024"]}],"id":[{"id":"10.13039\/501100001793","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP210101317"],"award-info":[{"award-number":["DP210101317"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Max Planck Queensland Centre on the Materials Science"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Bacterial polysaccharide synthesis is catalysed on the universal lipid carrier, undecaprenol phosphate (UndP). The cellular UndP pool is shared by different polysaccharide synthesis pathways including peptidoglycan biogenesis. Disruptions in cytosolic polysaccharide synthesis steps are detrimental to bacterial survival due to effects on UndP recycling. In contrast, bacteria can survive disruptions in the periplasmic steps, suggesting a tolerance mechanism to mitigate UndP sequestration. Here we investigated tolerance mechanisms to disruptions of polymerases that are involved in UndP-releasing steps in two related polysaccharide synthesis pathways: that for enterobacterial common antigen (ECA) and that for O antigen (OAg), in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic>Shigella flexneri<\/jats:italic>\n                    . Our study reveals that polysaccharide polymerisation is crucial for efficient UndP recycling. In\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>coli<\/jats:italic>\n                    K-12, cell survival upon disruptions in OAg polymerase is dependent on a functional ECA synthesis pathway and vice versa. This is because disruptions in OAg synthesis lead to the redirection of the shared lipid-linked sugar substrate UndPP-GlcNAc towards increased ECA production. Conversely, in\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>flexneri<\/jats:italic>\n                    , the OAg polymerase is essential due to its limited ECA production, which inadequately redirects UndP flow to support cell survival. We propose a model whereby sharing the initial sugar intermediate UndPP-GlcNAc between the ECA and OAg synthesis pathways allows UndP to be redirected towards ECA production, mitigating sequestration issues caused by disruptions in the OAg pathway. These findings suggest an evolutionary buffering mechanism that enhances bacterial survival when UndP sequestration occurs due to stalled polysaccharide biosynthesis, which may allow polysaccharide diversity in the species to increase over time.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011591","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T13:49:51Z","timestamp":1738244991000},"page":"e1011591","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Tolerance mechanisms in polysaccharide biosynthesis: Implications for undecaprenol phosphate recycling in Escherichia coli and Shigella flexneri"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5867-6332","authenticated-orcid":true,"given":"Jilong","family":"Qin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4408-2648","authenticated-orcid":true,"given":"Yaoqin","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas T.","family":"Maczuga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Morona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makrina","family":"Totsika","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"issue":"8","key":"pgen.1011591.ref001","doi-asserted-by":"crossref","first-page":"e71097","DOI":"10.1371\/journal.pone.0071097","article-title":"O-antigen protects gram-negative bacteria from histone killing.","volume":"8","author":"C Chaput","year":"2013","journal-title":"PloS one"},{"issue":"10","key":"pgen.1011591.ref002","doi-asserted-by":"crossref","first-page":"e1003834","DOI":"10.1371\/journal.pgen.1003834","article-title":"The serum resistome of a globally disseminated multidrug resistant uropathogenic Escherichia coli clone.","volume":"9","author":"MD Phan","year":"2013","journal-title":"PLoS Genet."},{"issue":"5508","key":"pgen.1011591.ref003","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1038\/255500a0","article-title":"Survival of orally administered E. coli K 12 in alimentary tract of man","volume":"255","author":"HW Smith","year":"1975","journal-title":"Nature"},{"issue":"52","key":"pgen.1011591.ref004","first-page":"E7266","article-title":"Glycan:glycan interactions: High affinity biomolecular interactions that can mediate binding of pathogenic bacteria to host cells","volume":"112","author":"CJ Day","year":"2015","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"5713","key":"pgen.1011591.ref005","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1126\/science.1108472","article-title":"Optimization of virulence functions through glucosylation of Shigella LPS","volume":"307","author":"NP West","year":"2005","journal-title":"Science"},{"issue":"6","key":"pgen.1011591.ref006","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1093\/femsre\/fuz028","article-title":"Structure and genetics of Escherichia coli O antigens","volume":"44","author":"B Liu","year":"2020","journal-title":"FEMS microbiology reviews"},{"issue":"1","key":"pgen.1011591.ref007","doi-asserted-by":"crossref","first-page":"71","DOI":"10.4049\/jimmunol.57.1.71","article-title":"The serology of the coli group","volume":"57","author":"F. Kauffmann","year":"1947","journal-title":"J Immunol"},{"issue":"3","key":"pgen.1011591.ref008","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.jchromb.2008.12.010","article-title":"Quantitative high-performance liquid chromatography analysis of the pool levels of undecaprenyl phosphate and its derivatives in bacterial membranes","volume":"877","author":"H Barreteau","year":"2009","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"issue":"3","key":"pgen.1011591.ref009","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1074\/jbc.M109.061630","article-title":"A novel epimerase that converts GlcNAc-P-P-undecaprenol to GalNAc-P-P-undecaprenol in Escherichia coli O157","volume":"285","author":"JS Rush","year":"2010","journal-title":"The Journal of biological chemistry"},{"key":"pgen.1011591.ref010","first-page":"140","article-title":"Escherichia coli K12 regains its O antigen","author":"D Liu","year":"1994","journal-title":"Microbiology (Reading)."},{"key":"pgen.1011591.ref011","article-title":"Repeat-Unit Elongations To Produce Bacterial Complex Long Polysaccharide Chains, an O-Antigen Perspective.","author":"Y Hong","year":"2023","journal-title":"EcoSal Plus."},{"issue":"4","key":"pgen.1011591.ref012","article-title":"Enterobacterial Common Antigen: Synthesis and Function of an Enigmatic Molecule.","volume":"11","author":"AK Rai","year":"2020","journal-title":"Mbio."},{"issue":"6","key":"pgen.1011591.ref013","doi-asserted-by":"crossref","first-page":"e0220323","DOI":"10.1128\/aem.02203-23","article-title":"Determining glycosyltransferase functional order via lethality due to accumulated O-antigen intermediates, exemplified with Shigella flexneri O-antigen biosynthesis","volume":"90","author":"J Qin","year":"2024","journal-title":"Appl Environ Microbiol"},{"issue":"4","key":"pgen.1011591.ref014","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1111\/j.1365-2958.2012.08048.x","article-title":"The Wzx translocases for Salmonella enterica O-antigen processing have unexpected serotype specificity","volume":"84","author":"Y Hong","year":"2012","journal-title":"Mol Microbiol"},{"issue":"8","key":"pgen.1011591.ref015","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1099\/mic.0.000113","article-title":"Three Wzy polymerases are specific for particular forms of an internal linkage in otherwise identical O units","volume":"161","author":"Y Hong","year":"2015","journal-title":"Microbiology (Reading)."},{"issue":"8","key":"pgen.1011591.ref016","doi-asserted-by":"crossref","first-page":"3545","DOI":"10.1128\/IAI.66.8.3545-3551.1998","article-title":"Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes","volume":"66","author":"L Wang","year":"1998","journal-title":"Infect Immun"},{"issue":"1","key":"pgen.1011591.ref017","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/mmi.13284","article-title":"Dead-end intermediates in the enterobacterial common antigen pathway induce morphological defects in Escherichia coli by competing for undecaprenyl phosphate","volume":"100","author":"MA Jorgenson","year":"2016","journal-title":"Mol Microbiol"},{"issue":"22","key":"pgen.1011591.ref018","doi-asserted-by":"crossref","first-page":"3070","DOI":"10.1128\/JB.00550-16","article-title":"Interrupting Biosynthesis of O Antigen or the Lipopolysaccharide Core Produces Morphological Defects in Escherichia coli by Sequestering Undecaprenyl Phosphate","volume":"198","author":"MA Jorgenson","year":"2016","journal-title":"J Bacteriol"},{"issue":"1","key":"pgen.1011591.ref019","doi-asserted-by":"crossref","first-page":"240310","DOI":"10.1098\/rsob.240310","article-title":"Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide","volume":"15","author":"A Le Bas","year":"2025","journal-title":"Open biology"},{"issue":"1","key":"pgen.1011591.ref020","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1128\/jb.100.1.433-444.1969","article-title":"Biosynthesis of cell wall lipopolysaccharide in mutants of Salmonella. V. A mutant of Salmonella typhimurium defective in the synthesis of cytidine diphosphoabequose","volume":"100","author":"R Yuasa","year":"1969","journal-title":"J Bacteriol"},{"issue":"9","key":"pgen.1011591.ref021","doi-asserted-by":"crossref","first-page":"3369","DOI":"10.1128\/JB.01938-06","article-title":"Mutations blocking side chain assembly, polymerization, or transport of a Wzy-dependent Streptococcus pneumoniae capsule are lethal in the absence of suppressor mutations and can affect polymer transfer to the cell wall","volume":"189","author":"B Xayarath","year":"2007","journal-title":"J Bacteriol"},{"issue":"7","key":"pgen.1011591.ref022","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.1128\/jb.178.7.2102-2107.1996","article-title":"An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase.","volume":"178","author":"D Liu","year":"1996","journal-title":"J Bacteriol"},{"issue":"10","key":"pgen.1011591.ref023","doi-asserted-by":"crossref","first-page":"e1010996","DOI":"10.1371\/journal.pgen.1010996","article-title":"O antigen biogenesis sensitises Escherichia coli K-12 to bile salts, providing a plausible explanation for its evolutionary loss.","volume":"19","author":"J Qin","year":"2023","journal-title":"PLoS Genet."},{"issue":"1","key":"pgen.1011591.ref024","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1186\/s12866-016-0818-0","article-title":"Combining Shigella Tn-seq data with gold-standard E. coli gene deletion data suggests rare transitions between essential and non-essential gene functionality","volume":"16","author":"NE Freed","year":"2016","journal-title":"BMC Microbiol"},{"issue":"19","key":"pgen.1011591.ref025","doi-asserted-by":"crossref","first-page":"16534","DOI":"10.1074\/jbc.M301750200","article-title":"Evidence that the wzxE gene of Escherichia coli K-12 encodes a protein involved in the transbilayer movement of a trisaccharide-lipid intermediate in the assembly of enterobacterial common antigen","volume":"278","author":"PD Rick","year":"2003","journal-title":"The Journal of biological chemistry"},{"key":"pgen.1011591.ref026","doi-asserted-by":"crossref","first-page":"e0179824","DOI":"10.1128\/mbio.01798-24","article-title":"High-throughput transposon mutagenesis in the family Enterobacteriaceae reveals core essential genes and rapid turnover of essentiality","volume":"2024","author":"FA Ghomi","year":"2024","journal-title":"Mbio"},{"issue":"4","key":"pgen.1011591.ref027","doi-asserted-by":"crossref","first-page":"e0054621","DOI":"10.1128\/jb.00546-21","article-title":"Interdependence of Shigella flexneri O Antigen and Enterobacterial Common Antigen Biosynthetic Pathways","volume":"204","author":"N Maczuga","year":"2022","journal-title":"J Bacteriol"},{"issue":"3","key":"pgen.1011591.ref028","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1128\/iai.18.3.563-567.1977","article-title":"Demonstration of enterobacterial common antigen by bacterial agglutination","volume":"18","author":"A Marx","year":"1977","journal-title":"Infect Immun"},{"issue":"2","key":"pgen.1011591.ref029","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1128\/jb.126.2.579-586.1976","article-title":"Role of a lipopolysaccharide gene for immunogenicity of the enterobacterial common antigen","volume":"126","author":"G Schmidt","year":"1976","journal-title":"J Bacteriol"},{"issue":"20","key":"pgen.1011591.ref030","doi-asserted-by":"crossref","first-page":"6917","DOI":"10.1128\/JB.187.20.6917-6927.2005","article-title":"Assembly of cyclic enterobacterial common antigen in Escherichia coli K-12","volume":"187","author":"J Kajimura","year":"2005","journal-title":"J Bacteriol"},{"issue":"3","key":"pgen.1011591.ref031","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1574-6968.1988.tb02743.x","article-title":"ECA, the enterobacterial common antigen","volume":"4","author":"HM Kuhn","year":"1988","journal-title":"FEMS microbiology reviews"},{"issue":"3","key":"pgen.1011591.ref032","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1128\/jb.176.3.733-747.1994","article-title":"Characterization of the rfc region of Shigella flexneri","volume":"176","author":"R Morona","year":"1994","journal-title":"J Bacteriol"},{"issue":"1","key":"pgen.1011591.ref033","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1128\/JB.01885-14","article-title":"Mutational analysis of the Shigella flexneri O-antigen polymerase Wzy: identification of Wzz-dependent Wzy mutants","volume":"197","author":"P Nath","year":"2015","journal-title":"J Bacteriol"},{"key":"pgen.1011591.ref034","first-page":"160","article-title":"Relationship between O-antigen chain length and resistance to colicin E2","author":"EN Tran","year":"2014","journal-title":"Shigella flexneri. Microbiology"},{"issue":"9","key":"pgen.1011591.ref035","doi-asserted-by":"crossref","first-page":"e0022422","DOI":"10.1128\/jb.00224-22","article-title":"Identification of the Shigella flexneri Wzy Domain Modulating Wzz(pHS-2) Interaction and Detection of the Wzy\/Wzz\/Oag Complex.","volume":"204","author":"A Ascari","year":"2022","journal-title":"J Bacteriol"},{"issue":"5","key":"pgen.1011591.ref036","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1111\/j.1365-2958.1993.tb01163.x","article-title":"Repeat unit polysaccharides of bacteria: a model for polymerization resembling that of ribosomes and fatty acid synthetase, with a novel mechanism for determining chain length","volume":"7","author":"DA Bastin","year":"1993","journal-title":"Mol Microbiol"},{"issue":"9","key":"pgen.1011591.ref037","doi-asserted-by":"crossref","first-page":"4008","DOI":"10.1128\/jb.170.9.4008-4014.1988","article-title":"Accumulation of a lipid-linked intermediate involved in enterobacterial common antigen synthesis in Salmonella typhimurium mutants lacking dTDP-glucose pyrophosphorylase","volume":"170","author":"PD Rick","year":"1988","journal-title":"J Bacteriol"},{"issue":"22","key":"pgen.1011591.ref038","doi-asserted-by":"crossref","first-page":"5875","DOI":"10.1128\/JB.180.22.5875-5884.1998","article-title":"Accumulation of the enterobacterial common antigen lipid II biosynthetic intermediate stimulates degP transcription in Escherichia coli","volume":"180","author":"PN Danese","year":"1998","journal-title":"J Bacteriol"},{"issue":"4","key":"pgen.1011591.ref039","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1021\/acschembio.0c00983","article-title":"Making the Enterobacterial Common Antigen Glycan and Measuring Its Substrate Sequestration.","volume":"16","author":"CR Eade","year":"2021","journal-title":"ACS Chem Biol"},{"key":"pgen.1011591.ref040","article-title":"Sequestration of dead-end undecaprenyl phosphate-linked oligosaccharide intermediate.","author":"Y Hong","year":"2024","journal-title":"bioRxiv"},{"issue":"1","key":"pgen.1011591.ref041","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00074-15","article-title":"Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase.","volume":"1","author":"Y Hong","year":"2016","journal-title":"Msphere."},{"issue":"6","key":"pgen.1011591.ref042","doi-asserted-by":"crossref","first-page":"e0324621","DOI":"10.1128\/mbio.03246-21","article-title":"A New Sugar for an Old Phage: a c-di-GMP-Dependent Polysaccharide Pathway Sensitizes Escherichia coli for Bacteriophage Infection.","volume":"12","author":"B Sellner","year":"2021","journal-title":"Mbio"},{"issue":"17","key":"pgen.1011591.ref043","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21176038","article-title":"Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA(LPS)) Occurs in Rough Strains of Escherichia coli R1, R2, and R4.","volume":"21","author":"A Maciejewska","year":"2020","journal-title":"Int J Mol Sci."},{"issue":"1","key":"pgen.1011591.ref044","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0003-2697(82)90673-X","article-title":"A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels","volume":"119","author":"CM Tsai","year":"1982","journal-title":"Anal Biochem"},{"issue":"19","key":"pgen.1011591.ref045","doi-asserted-by":"crossref","first-page":"5673","DOI":"10.1128\/JB.185.19.5673-5684.2003","article-title":"Experimental determination and system level analysis of essential genes in Escherichia coli MG1655","volume":"185","author":"SY Gerdes","year":"2003","journal-title":"J Bacteriol"},{"issue":"6","key":"pgen.1011591.ref046","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1128\/JB.184.6.1669-1677.2002","article-title":"Extensive variation in the O-antigen gene cluster within one Salmonella enterica serogroup reveals an unexpected complex history","volume":"184","author":"L Wang","year":"2002","journal-title":"J Bacteriol"},{"key":"pgen.1011591.ref047","doi-asserted-by":"crossref","DOI":"10.1038\/msb4100050","article-title":"Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection","volume":"2","author":"T Baba","year":"2006","journal-title":"Mol Syst Biol"},{"issue":"1","key":"pgen.1011591.ref048","article-title":"The Essential Genome of Escherichia coli K-12.","volume":"9","author":"ECA Goodall","year":"2018","journal-title":"Mbio"},{"key":"pgen.1011591.ref049","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.carbpol.2016.08.062","article-title":"Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus","volume":"156","author":"RR Sardari","year":"2017","journal-title":"Carbohydr Polym"},{"issue":"2","key":"pgen.1011591.ref050","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0092-8674(93)90418-P","article-title":"Biosynthesis of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti","volume":"74","author":"TL Reuber","year":"1993","journal-title":"Cell"},{"key":"pgen.1011591.ref051","article-title":"The Late-Stage Steps of Burkholderia cenocepacia Protein O-Linked Glycan Biosynthesis Are Conditionally Essential.","author":"L Jebeli","year":"2024","journal-title":"bioRxiv."},{"issue":"1","key":"pgen.1011591.ref052","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00154-17","article-title":"Progress in Our Understanding of Wzx Flippase for Translocation of Bacterial Membrane Lipid-Linked Oligosaccharide","volume":"200","author":"Y Hong","year":"2018","journal-title":"J Bacteriol"},{"issue":"4","key":"pgen.1011591.ref053","doi-asserted-by":"crossref","first-page":"e522","DOI":"10.5001\/omj.2023.65","article-title":"Shigella Flexneri Serotypes: O-antigen Structure, Serotype Conversion, and Serotyping Methods.","volume":"38","author":"M Sadredinamin","year":"2023","journal-title":"Oman Med J."},{"issue":"4","key":"pgen.1011591.ref054","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1139\/w04-010","article-title":"Deletion of the Escherichia coli O14:K7 O antigen gene cluster","volume":"50","author":"SO Jensen","year":"2004","journal-title":"Can J Microbiol"},{"issue":"2","key":"pgen.1011591.ref055","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/0042-6822(55)90016-7","article-title":"Transduction of linked genetic characters of the host by bacteriophage P1","volume":"1","author":"ES Lennox","year":"1955","journal-title":"Virology"},{"issue":"1","key":"pgen.1011591.ref056","doi-asserted-by":"crossref","first-page":"e0227425","DOI":"10.1371\/journal.pone.0227425","article-title":"The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function","volume":"15","author":"J Qin","year":"2020","journal-title":"PloS one"},{"issue":"1","key":"pgen.1011591.ref057","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0882-4010(03)00064-0","article-title":"The actin-based motility defect of a Shigella flexneri rmlD rough LPS mutant is not due to loss of IcsA polarity","volume":"35","author":"L Van den Bosch","year":"2003","journal-title":"Microb Pathog"},{"issue":"2","key":"pgen.1011591.ref058","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.plasmid.2005.07.001","article-title":"A family of arabinose-inducible Escherichia coli expression vectors having pBR322 copy control","volume":"55","author":"JE Cronan","year":"2006","journal-title":"Plasmid"},{"issue":"12","key":"pgen.1011591.ref059","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"KA Datsenko","year":"2000","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"19","key":"pgen.1011591.ref060","doi-asserted-by":"crossref","first-page":"16658","DOI":"10.1074\/jbc.M111.228221","article-title":"Functional and structural characterization of polysaccharide co-polymerase proteins required for polymer export in ATP-binding cassette transporter-dependent capsule biosynthesis pathways","volume":"286","author":"K Larue","year":"2011","journal-title":"The Journal of biological chemistry"},{"issue":"9","key":"pgen.1011591.ref061","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1128\/JB.01323-13","article-title":"Diversity of o-antigen repeat unit structures can account for the substantial sequence variation of wzx translocases","volume":"196","author":"Y Hong","year":"2014","journal-title":"J Bacteriol"},{"key":"pgen.1011591.ref062","author":"NT Maczuga","year":"2022","journal-title":"Enterobacterial Common Antigen Biosynthesis in Shigella flexneri"},{"issue":"4","key":"pgen.1011591.ref063","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1111\/mmi.14973","article-title":"Subcellular localization of the enterobacterial common antigen GT-E-like glycosyltransferase","volume":"118","author":"N Maczuga","year":"2022","journal-title":"WecG. Mol Microbiol"},{"issue":"1","key":"pgen.1011591.ref064","doi-asserted-by":"crossref","first-page":"11629","DOI":"10.1038\/s41598-022-15997-8","article-title":"A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide.","volume":"12","author":"J Qin","year":"2022","journal-title":"Scientific reports."}],"updated-by":[{"DOI":"10.1371\/journal.pgen.1011591","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000}}],"container-title":["PLOS Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1011591","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T13:48:41Z","timestamp":1739281721000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1011591"}},"subtitle":[],"editor":[{"given":"Jue D.","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,1,30]]},"references-count":64,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pgen.1011591","relation":{},"ISSN":["1553-7404"],"issn-type":[{"value":"1553-7404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,30]]}}}