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HIP14 and HIP14L ankyrin repeat-containing palmitoyl transferases were linked to the pathogenesis of Huntington\u2019s disease, however, how perturbation of these Golgi resident enzymes contributes to neurological disorders is yet to be understood. In this study, we investigated the function of Hip14 and Patsas - the Drosophila orthologs of HIP14 and HIP14L, respectively \u2013 to uncover their role in secretory and lysosomal membrane trafficking. Using larval salivary gland, a well-established model of the regulated secretory pathway, we found that these PAT enzymes equally contribute to the proper maturation and crinophagic degradation of glue secretory granules by mediating their fusion with the endo-lysosomal compartment. We also revealed that Patsas and Hip14 are both required for lysosomal acidification and biosynthetic transport of various lysosomal hydrolases, and we demonstrated that the rate of secretory granule-lysosome fusion and subsequent acidification positively correlates with the level of Hip14. Furthermore, Hip14 is also essential for proper lysosome morphology and neuronal function in adult brains. Finally, we found that the over-activation of lysosomal biosynthetic transport and lysosomal fusions by the expression of the constitutively active form of Rab2 could compensate for the lysosomal dysfunction caused by the loss of Patsas or Hip14 both in larval salivary glands and neurons. Therefore, we propose that ankyrin repeat palmitoyl transferases act as rate-limiting factors in lysosomal fusions and provide genetic evidence that defective protein palmitoylation and the subsequent lysosomal dysfunction can contribute to the onset of Huntington\u2019s disease-like symptoms.<\/jats:p>","DOI":"10.1371\/journal.pgen.1011607","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T18:50:31Z","timestamp":1767207031000},"page":"e1011607","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Huntington\u2019s disease-associated ankyrin repeat palmitoyl transferases are rate-limiting factors in lysosome formation and fusion"],"prefix":"10.1371","volume":"21","author":[{"given":"Gy\u0151z\u0151","family":"Szenci","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1811-8595","authenticated-orcid":true,"given":"Attila","family":"Boda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0979-1617","authenticated-orcid":true,"given":"Anik\u00f3","family":"Nagy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9675-8882","authenticated-orcid":true,"given":"Dorottya","family":"K\u00e1rolyi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7370-6220","authenticated-orcid":true,"given":"Andr\u00e1s","family":"Rubics","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2413-3627","authenticated-orcid":true,"given":"Zsombor","family":"Sz\u0151ke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0256-3275","authenticated-orcid":true,"given":"Gerg\u0151","family":"Falcsik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tibor","family":"Kov\u00e1cs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7374-667X","authenticated-orcid":true,"given":"P\u00e9ter","family":"L\u0151rincz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8548-8874","authenticated-orcid":true,"given":"G\u00e1bor","family":"Juh\u00e1sz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2139-7740","authenticated-orcid":true,"given":"Szabolcs","family":"Tak\u00e1ts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"issue":"5","key":"pgen.1011607.ref001","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.tibs.2011.01.003","article-title":"DHHC 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