{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:56:29Z","timestamp":1787021789801,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011616","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000}}],"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T00:00:00Z","timestamp":1743379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"NIGMS","doi-asserted-by":"crossref","award":["R01GM084376"],"award-info":[{"award-number":["R01GM084376"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    The Drosophila Pumilio (Pum) and Nanos (Nos) RNA-binding proteins govern abdominal segmentation in the early embryo, as well as a variety of other events during development. They bind together to a compound Nanos Response Element (NRE) present in thousands of maternal mRNAs in the ovary and embryo, including\n                    <jats:italic>hunchback<\/jats:italic>\n                    (\n                    <jats:italic>hb<\/jats:italic>\n                    ) mRNA, thereby regulating poly-adenylation, translation, and stability. Many studies support a model in which mRNA recognition and effector recruitment are carried out by distinct regions of each protein. The well-ordered Pum and Nos RNA-binding domains (RBDs) are sufficient to specifically recognize NREs; the larger intrinsically disordered N-terminal regions (NTRs) of each protein have been thought to act by recruiting mRNA regulators. Here we use yeast interaction assays and experiments testing the regulation of\n                    <jats:italic>hb<\/jats:italic>\n                    mRNA in vivo to show that the NTRs play a significant role in recognition of the NRE, acting via two mechanisms. First, the Pum and Nos NTRs interact in trans to promote assembly of the Pum\/Nos\/NRE ternary complex. Second, the Pum NTR acts via an unknown mechanism in cis, modifying NRE recognition by its RBD. The ability of the NTR to alter binding to the NRE is conserved in human Pum2.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011616","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T16:40:22Z","timestamp":1743439222000},"page":"e1011616","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Significant roles in RNA-binding for the amino-terminal regions of Drosophila Pumilio and Nanos"],"prefix":"10.1371","volume":"21","author":[{"given":"Tammy H.","family":"Wharton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Marhabaie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8506-1379","authenticated-orcid":true,"given":"Robin P.","family":"Wharton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,3,31]]},"reference":[{"issue":"4","key":"pgen.1011616.ref001","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1242\/dev.125.4.679","article-title":"Nanos and Pumilio have critical roles in the development and function of Drosophila germline stem cells","volume":"125","author":"A Forbes","year":"1998","journal-title":"Development"},{"issue":"12","key":"pgen.1011616.ref002","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1242\/dev.124.12.2463","article-title":"A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary","volume":"124","author":"H Lin","year":"1997","journal-title":"Development"},{"issue":"10","key":"pgen.1011616.ref003","doi-asserted-by":"crossref","first-page":"e3002840","DOI":"10.1371\/journal.pbio.3002840","article-title":"Widespread regulation of the maternal transcriptome by Nanos in Drosophila","volume":"22","author":"M Marhabaie","year":"2024","journal-title":"PLoS Biol"},{"issue":"20","key":"pgen.1011616.ref004","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.1101\/gad.13.20.2704","article-title":"Recruitment of nanos to hunchback mRNA by Pumilio","volume":"13","author":"J Sonoda","year":"1999","journal-title":"Genes Dev"},{"key":"pgen.1011616.ref005","doi-asserted-by":"crossref","first-page":"e17096","DOI":"10.7554\/eLife.17096","article-title":"Drosophila Nanos acts as a molecular clamp that modulates the RNA-binding and repression activities of Pumilio","volume":"5","author":"CA Weidmann","year":"2016","journal-title":"Elife"},{"issue":"5","key":"pgen.1011616.ref006","doi-asserted-by":"crossref","first-page":"e1008090","DOI":"10.1371\/journal.pgen.1008090","article-title":"Maternal Nanos inhibits Importin-\u03b12\/Pendulin-dependent nuclear import to prevent somatic gene expression in the Drosophila germline","volume":"15","author":"M Asaoka","year":"2019","journal-title":"PLoS Genet"},{"issue":"7","key":"pgen.1011616.ref007","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/15666","article-title":"Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos","volume":"1","author":"M Asaoka-Taguchi","year":"1999","journal-title":"Nat Cell Biol"},{"issue":"18","key":"pgen.1011616.ref008","doi-asserted-by":"crossref","first-page":"7455","DOI":"10.1073\/pnas.0610052104","article-title":"Maternal Nanos represses hid\/skl-dependent apoptosis to maintain the germ line in Drosophila embryos","volume":"104","author":"K Sato","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"10","key":"pgen.1011616.ref009","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.cub.2008.04.033","article-title":"Spatial regulation of nanos is required for its function in dendrite morphogenesis","volume":"18","author":"JL Brechbiel","year":"2008","journal-title":"Curr Biol"},{"issue":"4","key":"pgen.1011616.ref010","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.cub.2004.01.052","article-title":"Nanos and Pumilio are essential for dendrite morphogenesis in Drosophila peripheral neurons","volume":"14","author":"B Ye","year":"2004","journal-title":"Curr Biol"},{"issue":"17","key":"pgen.1011616.ref011","doi-asserted-by":"crossref","first-page":"5558","DOI":"10.1523\/JNEUROSCI.0520-09.2009","article-title":"The translational repressors Nanos and Pumilio have divergent effects on presynaptic terminal growth and postsynaptic glutamate receptor subunit composition","volume":"29","author":"KP Menon","year":"2009","journal-title":"J Neurosci"},{"issue":"4","key":"pgen.1011616.ref012","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.neuron.2004.10.028","article-title":"The translational repressor Pumilio regulates presynaptic morphology and controls postsynaptic accumulation of translation factor eIF-4E","volume":"44","author":"KP Menon","year":"2004","journal-title":"Neuron"},{"issue":"6","key":"pgen.1011616.ref013","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/S1097-2765(00)80085-4","article-title":"The Pumilio RNA-binding domain is also a translational regulator","volume":"1","author":"RP Wharton","year":"1998","journal-title":"Mol Cell"},{"issue":"12","key":"pgen.1011616.ref014","first-page":"1421","article-title":"The Pumilio protein binds RNA through a conserved domain that defines a new class of RNA-binding proteins","volume":"3","author":"PD Zamore","year":"1997","journal-title":"RNA"},{"issue":"4","key":"pgen.1011616.ref015","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0092-8674(02)00873-5","article-title":"Modular recognition of RNA by a human pumilio-homology domain","volume":"110","author":"X Wang","year":"2002","journal-title":"Cell"},{"issue":"4","key":"pgen.1011616.ref016","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1093\/emboj\/16.4.834","article-title":"A CCHC metal-binding domain in Nanos is essential for translational regulation","volume":"16","author":"D Curtis","year":"1997","journal-title":"EMBO J"},{"issue":"4","key":"pgen.1011616.ref017","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1093\/nar\/gkz1187","article-title":"Unique repression domains of Pumilio utilize deadenylation and decapping factors to accelerate destruction of target mRNAs","volume":"48","author":"RM Arvola","year":"2020","journal-title":"Nucleic Acids Res"},{"issue":"16","key":"pgen.1011616.ref018","doi-asserted-by":"crossref","first-page":"8496","DOI":"10.1073\/pnas.93.16.8496","article-title":"A three-hybrid system to detect RNA-protein interactions in vivo","volume":"93","author":"DJ SenGupta","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"pgen.1011616.ref019","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1016\/j.molcel.2019.04.012","article-title":"A quantitative and predictive model for RNA binding by human pumilio proteins","volume":"74","author":"I Jarmoskaite","year":"2019","journal-title":"Mol Cell"},{"issue":"8","key":"pgen.1011616.ref020","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1242\/dev.002212","article-title":"Translational control of maternal Cyclin B mRNA by Nanos in the Drosophila germline","volume":"134","author":"LY Kadyrova","year":"2007","journal-title":"Development"},{"issue":"9","key":"pgen.1011616.ref021","doi-asserted-by":"crossref","first-page":"974","DOI":"10.15252\/embj.201593634","article-title":"Distinct modes of recruitment of the CCR4-NOT complex by Drosophila and vertebrate Nanos","volume":"35","author":"T Raisch","year":"2016","journal-title":"EMBO J"},{"issue":"9","key":"pgen.1011616.ref022","doi-asserted-by":"crossref","first-page":"102270","DOI":"10.1016\/j.jbc.2022.102270","article-title":"A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs","volume":"298","author":"RJ Haugen","year":"2022","journal-title":"J Biol Chem"},{"issue":"8","key":"pgen.1011616.ref023","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1261\/rna.046029.114","article-title":"The RNA binding domain of Pumilio antagonizes poly-adenosine binding protein and accelerates deadenylation","volume":"20","author":"CA Weidmann","year":"2014","journal-title":"RNA"},{"issue":"2","key":"pgen.1011616.ref024","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0092-8674(01)00318-X","article-title":"Structure of Pumilio reveals similarity between RNA and peptide binding motifs","volume":"105","author":"TA Edwards","year":"2001","journal-title":"Cell"},{"key":"pgen.1011616.ref025","doi-asserted-by":"crossref","first-page":"e43788","DOI":"10.7554\/eLife.43788","article-title":"Engineering a conserved RNA regulatory protein repurposes its biological function in vivo","volume":"8","author":"VD Bhat","year":"2019","journal-title":"Elife"},{"issue":"2","key":"pgen.1011616.ref026","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1261\/rna.7202705","article-title":"RNA-protein interactions in the yeast three-hybrid system: affinity, sensitivity, and enhanced library screening","volume":"11","author":"B Hook","year":"2005","journal-title":"RNA"},{"issue":"1","key":"pgen.1011616.ref027","doi-asserted-by":"crossref","first-page":"5107","DOI":"10.1038\/s41467-021-25433-6","article-title":"Expanding the binding specificity for RNA recognition by a PUF domain","volume":"12","author":"W Zhou","year":"2021","journal-title":"Nat Commun"},{"issue":"4","key":"pgen.1011616.ref028","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1261\/rna.7255805","article-title":"Binding specificity and mRNA targets of a C. elegans PUF protein, FBF-1","volume":"11","author":"D Bernstein","year":"2005","journal-title":"RNA"},{"issue":"23","key":"pgen.1011616.ref029","doi-asserted-by":"crossref","first-page":"10510","DOI":"10.1073\/pnas.88.23.10510","article-title":"GAL4 is phosphorylated as a consequence of transcriptional activation","volume":"88","author":"I Sadowski","year":"1991","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"9","key":"pgen.1011616.ref030","doi-asserted-by":"crossref","first-page":"4879","DOI":"10.1128\/MCB.16.9.4879","article-title":"Phosphorylation of Ga14p at a single C-terminal residue is necessary for galactose-inducible transcription","volume":"16","author":"I Sadowski","year":"1996","journal-title":"Mol Cell Biol"},{"issue":"1","key":"pgen.1011616.ref031","doi-asserted-by":"crossref","first-page":"7323","DOI":"10.1038\/s41467-023-43098-1","article-title":"Intra- and inter-molecular regulation by intrinsically-disordered regions governs PUF protein RNA binding","volume":"14","author":"C Qiu","year":"2023","journal-title":"Nat Commun"},{"issue":"12","key":"pgen.1011616.ref032","doi-asserted-by":"crossref","first-page":"4487","DOI":"10.1073\/pnas.0509260103","article-title":"Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster","volume":"103","author":"AP Gerber","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"pgen.1011616.ref033","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1038\/s41580-020-00326-6","article-title":"Biomolecular condensates at the nexus of cellular stress, protein aggregation disease and ageing","volume":"22","author":"S Alberti","year":"2021","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"2","key":"pgen.1011616.ref034","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0092-8674(92)90358-J","article-title":"Localization of nanos RNA controls embryonic polarity","volume":"71","author":"ER Gavis","year":"1992","journal-title":"Cell"},{"issue":"5","key":"pgen.1011616.ref035","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/0092-8674(89)90611-9","article-title":"Structure of the Drosophila BicaudalD protein and its role in localizing the posterior determinant nanos","volume":"59","author":"RP Wharton","year":"1989","journal-title":"Cell"},{"issue":"5","key":"pgen.1011616.ref036","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/0092-8674(91)90368-9","article-title":"RNA regulatory elements mediate control of Drosophila body pattern by the posterior morphogen nanos","volume":"67","author":"RP Wharton","year":"1991","journal-title":"Cell"},{"issue":"6","key":"pgen.1011616.ref037","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1101\/gad.870801","article-title":"Drosophila Brain Tumor is a translational repressor","volume":"15","author":"J Sonoda","year":"2001","journal-title":"Genes Dev"},{"issue":"7","key":"pgen.1011616.ref038","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1101\/gad.236513.113","article-title":"The NHL domain of BRAT is an RNA-binding domain that directly contacts the hunchback mRNA for regulation","volume":"28","author":"I Loedige","year":"2014","journal-title":"Genes Dev"},{"issue":"5","key":"pgen.1011616.ref039","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/0092-8674(95)90353-4","article-title":"Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos","volume":"80","author":"Y Murata","year":"1995","journal-title":"Cell"},{"issue":"3","key":"pgen.1011616.ref040","doi-asserted-by":"crossref","first-page":"e0194865","DOI":"10.1371\/journal.pone.0194865","article-title":"No significant regulation of bicoid mRNA by Pumilio or Nanos in the early Drosophila embryo","volume":"13","author":"TH Wharton","year":"2018","journal-title":"PLoS One"},{"issue":"9","key":"pgen.1011616.ref041","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1074\/mcp.RA119.001912","article-title":"An Interaction Network of RNA-Binding Proteins Involved in Drosophila Oogenesis","volume":"19","author":"P Bansal","year":"2020","journal-title":"Mol Cell Proteomics"},{"issue":"6217","key":"pgen.1011616.ref042","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/338629a0","article-title":"Posterior segmentation of the Drosophila embryo in the absence of a maternal posterior organizer gene","volume":"338","author":"M H\u00fclskamp","year":"1989","journal-title":"Nature"},{"issue":"6217","key":"pgen.1011616.ref043","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1038\/338646a0","article-title":"The Drosophila posterior-group gene nanos functions by repressing hunchback activity","volume":"338","author":"V Irish","year":"1989","journal-title":"Nature"},{"issue":"6218","key":"pgen.1011616.ref044","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1038\/338741a0","article-title":"Differing strategies for organizing anterior and posterior body pattern in Drosophila embryos","volume":"338","author":"G Struhl","year":"1989","journal-title":"Nature"},{"issue":"6","key":"pgen.1011616.ref045","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1016\/j.celrep.2015.09.068","article-title":"The crystal structure of the NHL domain in complex with RNA reveals the molecular basis of drosophila brain-tumor-mediated gene regulation","volume":"13","author":"I Loedige","year":"2015","journal-title":"Cell Rep"},{"issue":"11","key":"pgen.1011616.ref046","doi-asserted-by":"crossref","first-page":"3699","DOI":"10.1002\/j.1460-2075.1990.tb07582.x","article-title":"The Xenopus laevis poly(A) binding protein is composed of multiple functionally independent RNA binding domains","volume":"9","author":"W Nietfeld","year":"1990","journal-title":"EMBO J"},{"issue":"6","key":"pgen.1011616.ref047","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/S0092-8674(00)81517-2","article-title":"Recognition of polyadenylate RNA by the poly(A)-binding protein","volume":"98","author":"RC Deo","year":"1999","journal-title":"Cell"},{"issue":"3","key":"pgen.1011616.ref048","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.molcel.2018.11.012","article-title":"The solution structure of FUS bound to RNA reveals a bipartite mode of RNA recognition with both sequence and shape specificity","volume":"73","author":"FE Loughlin","year":"2019","journal-title":"Mol Cell"},{"key":"pgen.1011616.ref049","doi-asserted-by":"crossref","first-page":"e48968","DOI":"10.7554\/eLife.48968","article-title":"A crystal structure of a collaborative RNA regulatory complex reveals mechanisms to refine target specificity","volume":"8","author":"C Qiu","year":"2019","journal-title":"Elife"},{"issue":"7873","key":"pgen.1011616.ref050","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"J Jumper","year":"2021","journal-title":"Nature"},{"issue":"D1","key":"pgen.1011616.ref051","doi-asserted-by":"crossref","first-page":"D439","DOI":"10.1093\/nar\/gkab1061","article-title":"AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models","volume":"50","author":"M Varadi","year":"2022","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"pgen.1011616.ref052","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1261\/rna.079008.121","article-title":"Using quantitative reconstitution to investigate multicomponent condensates","volume":"28","author":"SL Currie","year":"2022","journal-title":"RNA"},{"issue":"2","key":"pgen.1011616.ref053","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1523\/JNEUROSCI.2523-09.2010","article-title":"Regulation of synaptic Pumilio function by an aggregation-prone domain","volume":"30","author":"AM Salazar","year":"2010","journal-title":"J Neurosci"},{"issue":"24","key":"pgen.1011616.ref054","doi-asserted-by":"crossref","first-page":"6496","DOI":"10.1523\/JNEUROSCI.0649-06.2006","article-title":"Dendritic localization of the translational repressor Pumilio 2 and its contribution to dendritic stress granules","volume":"26","author":"JP Vessey","year":"2006","journal-title":"J Neurosci"},{"issue":"11","key":"pgen.1011616.ref055","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1038\/embor.2010.155","article-title":"Crystal structure of zinc-finger domain of Nanos and its functional implications","volume":"11","author":"H Hashimoto","year":"2010","journal-title":"EMBO Rep"},{"issue":"1","key":"pgen.1011616.ref056","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s00018-018-2926-5","article-title":"PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues","volume":"76","author":"M Sajek","year":"2019","journal-title":"Cell Mol Life Sci"},{"issue":"1","key":"pgen.1011616.ref057","doi-asserted-by":"crossref","first-page":"37","DOI":"10.15252\/embr.201540828","article-title":"Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development","volume":"17","author":"A Suzuki","year":"2016","journal-title":"EMBO Rep"},{"issue":"1","key":"pgen.1011616.ref058","doi-asserted-by":"crossref","first-page":"9625","DOI":"10.1038\/s41598-022-13089-1","article-title":"Design of typical genes for heterologous gene expression","volume":"12","author":"D Simm","year":"2022","journal-title":"Sci Rep"},{"issue":"13","key":"pgen.1011616.ref059","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1093\/nar\/gkg595","article-title":"Mfold web server for nucleic acid folding and hybridization prediction","volume":"31","author":"M Zuker","year":"2003","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"pgen.1011616.ref060","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1093\/genetics\/144.4.1425","article-title":"Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast","volume":"144","author":"P James","year":"1996","journal-title":"Genetics"},{"issue":"10","key":"pgen.1011616.ref061","doi-asserted-by":"crossref","first-page":"e1078","DOI":"10.1371\/journal.pone.0001078","article-title":"Optimized protein extraction for quantitative proteomics of yeasts","volume":"2","author":"T von der Haar","year":"2007","journal-title":"PLoS One"},{"issue":"W1","key":"pgen.1011616.ref062","doi-asserted-by":"crossref","first-page":"W329","DOI":"10.1093\/nar\/gky384","article-title":"IUPred2A: context-dependent prediction of protein disorder as a function of redox state and protein binding","volume":"46","author":"B M\u00e9sz\u00e1ros","year":"2018","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"pgen.1011616.ref063","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1002\/aja.1001990204","article-title":"Genetics of nanos localization in Drosophila","volume":"199","author":"C Wang","year":"1994","journal-title":"Dev Dyn"}],"updated-by":[{"DOI":"10.1371\/journal.pgen.1011616","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000}}],"container-title":["PLOS Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1011616","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T13:48:56Z","timestamp":1744206536000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1011616"}},"subtitle":[],"editor":[{"given":"Elizabeth R.","family":"Gavis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,31]]},"references-count":63,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pgen.1011616","relation":{},"ISSN":["1553-7404"],"issn-type":[{"value":"1553-7404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,31]]}}}