{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T02:10:55Z","timestamp":1783476655525,"version":"3.55.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011617","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000}}],"reference-count":75,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["GA642934"],"award-info":[{"award-number":["GA642934"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>Friend of GATA1 (FOG-1) is an essential transcriptional co-factor of the master erythroid transcription factor GATA1. The knockout of the <jats:italic>Zfpm1<\/jats:italic> gene, coding for FOG-1, results in early embryonic lethality due to anemia in mice, similar to the embryonic lethal phenotype of the <jats:italic>Gata1<\/jats:italic> gene knockout. However, a detailed molecular analysis of the <jats:italic>Zfpm1<\/jats:italic> knockout phenotype in erythropoiesis is presently incomplete. To this end, we used CRISPR\/Cas9 to knockout <jats:italic>Zfpm1<\/jats:italic> in mouse erythroleukemic (MEL) cells. Phenotypic characterization of DMSO-induced terminal erythroid differentiation showed that the <jats:italic>Zfpm1<\/jats:italic> knockout MEL cells did not progress past the proerythroblast stage of differentiation. Expression profiling of the <jats:italic>Zfpm1<\/jats:italic> knockout MEL cells by RNAseq showed a lack of\u00a0up-regulation\u00a0of erythroid-related gene expression profiles. Bioinformatic analysis highlighted cholesterol transport as a pathway affected in the <jats:italic>Zfpm1<\/jats:italic> knockout cells. Moreover, we show that the cholesterol transporters <jats:italic>Abca1<\/jats:italic> and <jats:italic>Ldlr<\/jats:italic> fail to be repressed during erythroid differentiation in <jats:italic>Zfpm1<\/jats:italic> knockout cells, resulting in higher intracellular lipid levels and higher membrane fluidity. We also show that in FOG-1 knockout cells, the nuclear levels of SREBP2, a key transcriptional regulator of cholesterol biosynthesis and transport, are markedly increased. On the basis of these findings we propose that FOG-1 (and, potentially, GATA1) regulate cholesterol homeostasis during erythroid differentiation directly through the\u00a0down regulation\u00a0of cholesterol transport genes and indirectly, through the repression of the SREBP2 transcriptional activator of cholesterol homeostasis. Taken together, our work provides a molecular basis for understanding FOG-1 functions in\u00a0erythropoiesis\u00a0and reveals a novel role for FOG-1 in cholesterol transport.<\/jats:p>","DOI":"10.1371\/journal.pgen.1011617","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T18:22:37Z","timestamp":1741285357000},"page":"e1011617","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["A novel role for Friend of GATA1 (FOG-1) in regulating cholesterol transport in murine erythropoiesis"],"prefix":"10.1371","volume":"21","author":[{"given":"Ioannis-Marios","family":"Roussis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8747-7554","authenticated-orcid":true,"given":"David J.","family":"Pearton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7176-8883","authenticated-orcid":true,"given":"Umar","family":"Niazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grigorios","family":"Tsaknakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio L.","family":"Papadopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5212-6057","authenticated-orcid":true,"given":"Riley","family":"Cook","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansoor","family":"Saqi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8515-0934","authenticated-orcid":true,"given":"Jiannis","family":"Ragoussis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6133-2872","authenticated-orcid":true,"given":"John","family":"Strouboulis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,3,6]]},"reference":[{"issue":"21","key":"pgen.1011617.ref001","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.1242\/dev.080440","article-title":"Combinatorial regulation of tissue specification by GATA and FOG factors","volume":"139","author":"TM Chlon","year":"2012","journal-title":"Development"},{"issue":"1","key":"pgen.1011617.ref002","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0092-8674(00)80318-9","article-title":"FOG, a multitype zinc finger protein, acts as a cofactor for transcription factor GATA-1 in erythroid and megakaryocytic differentiation","volume":"90","author":"AP Tsang","year":"1997","journal-title":"Cell"},{"issue":"8","key":"pgen.1011617.ref003","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1101\/gad.12.8.1176","article-title":"Failure of megakaryopoiesis and arrested erythropoiesis in mice lacking the GATA-1 transcriptional cofactor FOG","volume":"12","author":"AP Tsang","year":"1998","journal-title":"Genes Dev"},{"issue":"13","key":"pgen.1011617.ref004","doi-asserted-by":"crossref","first-page":"2354","DOI":"10.1038\/sj.emboj.7600702","article-title":"GATA-1 forms distinct activating and repressive complexes in erythroid cells","volume":"24","author":"P Rodriguez","year":"2005","journal-title":"EMBO J"},{"issue":"13","key":"pgen.1011617.ref005","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1038\/sj.emboj.7600703","article-title":"FOG-1 recruits the NuRD repressor complex to mediate transcriptional repression by GATA-1","volume":"24","author":"W Hong","year":"2005","journal-title":"EMBO J"},{"issue":"9","key":"pgen.1011617.ref006","doi-asserted-by":"crossref","first-page":"3121","DOI":"10.1128\/MCB.22.9.3121-3128.2002","article-title":"Interaction between FOG-1 and the corepressor C-terminal binding protein is dispensable for normal erythropoiesis in vivo","volume":"22","author":"SG Katz","year":"2002","journal-title":"Mol Cell Biol"},{"issue":"8","key":"pgen.1011617.ref007","doi-asserted-by":"crossref","first-page":"e106011","DOI":"10.1371\/journal.pone.0106011","article-title":"The identification and structure of an N-terminal PR domain show that FOG1 is a member of the PRDM family of proteins","volume":"9","author":"MK Clifton","year":"2014","journal-title":"PLoS One"},{"issue":"43","key":"pgen.1011617.ref008","doi-asserted-by":"crossref","first-page":"29310","DOI":"10.1074\/jbc.M109.043497","article-title":"Translational isoforms of FOG1 regulate GATA1-interacting complexes","volume":"284","author":"J Snow","year":"2009","journal-title":"J Biol Chem"},{"issue":"1","key":"pgen.1011617.ref009","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1242\/dev.121.1.163","article-title":"Development of hematopoietic cells lacking transcription factor GATA-1","volume":"121","author":"L Pevny","year":"1995","journal-title":"Development"},{"issue":"6306","key":"pgen.1011617.ref010","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/349257a0","article-title":"Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1","volume":"349","author":"L Pevny","year":"1991","journal-title":"Nature"},{"issue":"9","key":"pgen.1011617.ref011","doi-asserted-by":"crossref","first-page":"2867","DOI":"10.1182\/blood.V93.9.2867.409k24_2867_2875","article-title":"Consequences of GATA-1 Deficiency in Megakaryocytes and Platelets","volume":"93","author":"P Vyas","year":"1999","journal-title":"Blood"},{"issue":"13","key":"pgen.1011617.ref012","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.1093\/emboj\/16.13.3965","article-title":"A lineage-selective knockout establishes the critical role of transcription factor GATA-1 in megakaryocyte growth and platelet development","volume":"16","author":"RA Shivdasani","year":"1997","journal-title":"EMBO J"},{"issue":"2","key":"pgen.1011617.ref013","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S1097-2765(00)80312-3","article-title":"Use of altered specificity mutants to probe a specific protein-protein interaction in differentiation: the GATA-1:FOG complex","volume":"3","author":"JD Crispino","year":"1999","journal-title":"Mol Cell"},{"issue":"14","key":"pgen.1011617.ref014","doi-asserted-by":"crossref","first-page":"9237","DOI":"10.1073\/pnas.142302099","article-title":"GATA-factor dependence of the multitype zinc-finger protein FOG-1 for its essential role in megakaryopoiesis","volume":"99","author":"AN Chang","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"pgen.1011617.ref015","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/73480","article-title":"Familial dyserythropoietic anaemia and thrombocytopenia due to an inherited mutation in GATA1","volume":"24","author":"KE Nichols","year":"2000","journal-title":"Nat Genet"},{"issue":"4","key":"pgen.1011617.ref016","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1073\/pnas.0307612100","article-title":"Coregulator-dependent facilitation of chromatin occupancy by GATA-1","volume":"101","author":"S Pal","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"pgen.1011617.ref017","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1073\/pnas.0306315101","article-title":"Context-dependent regulation of GATA-1 by friend of GATA-1","volume":"101","author":"DL Letting","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"pgen.1011617.ref018","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.molcel.2012.05.051","article-title":"Cofactor-mediated restriction of GATA-1 chromatin occupancy coordinates lineage-specific gene expression","volume":"47","author":"TM Chlon","year":"2012","journal-title":"Mol Cell"},{"issue":"3","key":"pgen.1011617.ref019","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.molcel.2004.12.028","article-title":"Proximity among distant regulatory elements at the beta-globin locus requires GATA-1 and FOG-1","volume":"17","author":"CR Vakoc","year":"2005","journal-title":"Mol Cell"},{"issue":"2","key":"pgen.1011617.ref020","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.molcel.2007.11.020","article-title":"Exchange of GATA factors mediates transitions in looped chromatin organization at a developmentally regulated gene locus","volume":"29","author":"H Jing","year":"2008","journal-title":"Mol Cell"},{"issue":"2","key":"pgen.1011617.ref021","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1038\/emboj.2009.336","article-title":"NuRD mediates activating and repressive functions of GATA-1 and FOG-1 during blood development","volume":"29","author":"A Miccio","year":"2010","journal-title":"EMBO J"},{"issue":"22","key":"pgen.1011617.ref022","doi-asserted-by":"crossref","first-page":"23597","DOI":"10.1074\/jbc.M313987200","article-title":"Transforming acidic coiled-coil protein 3 (TACC3) controls friend of GATA-1 (FOG-1) subcellular localization and regulates the association between GATA-1 and FOG-1 during hematopoiesis","volume":"279","author":"M Garriga-Canut","year":"2004","journal-title":"J Biol Chem"},{"issue":"41","key":"pgen.1011617.ref023","doi-asserted-by":"crossref","first-page":"17413","DOI":"10.1073\/pnas.0909296106","article-title":"Resolving the distinct stages in erythroid differentiation based on dynamic changes in membrane protein expression during erythropoiesis","volume":"106","author":"K Chen","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"pgen.1011617.ref024","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1182\/blood.V94.1.87.413k41_87_96","article-title":"GATA-1 and erythropoietin cooperate to promote erythroid cell survival by regulating bcl-xL expression","volume":"94","author":"T Gregory","year":"1999","journal-title":"Blood"},{"issue":"3","key":"pgen.1011617.ref025","first-page":"655","article-title":"GATA-binding protein 1 is a novel transcription regulator of peroxiredoxin 5 in human breast cancer cells","volume":"40","author":"MJ Seo","year":"2012","journal-title":"Int J Oncol"},{"issue":"8","key":"pgen.1011617.ref026","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: a graphical gene-set enrichment tool for animals and plants","volume":"36","author":"SX Ge","year":"2020","journal-title":"Bioinformatics"},{"issue":"19","key":"pgen.1011617.ref027","doi-asserted-by":"crossref","first-page":"2438","DOI":"10.1093\/bioinformatics\/btq466","article-title":"ChEA: transcription factor regulation inferred from integrating genome-wide ChIP-X experiments","volume":"26","author":"A Lachmann","year":"2010","journal-title":"Bioinformatics"},{"issue":"43","key":"pgen.1011617.ref028","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"A Subramanian","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"pgen.1011617.ref029","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/ng1180","article-title":"PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes","volume":"34","author":"VK Mootha","year":"2003","journal-title":"Nat Genet"},{"issue":"14","key":"pgen.1011617.ref030","doi-asserted-by":"crossref","first-page":"5031","DOI":"10.1128\/MCB.23.14.5031-5042.2003","article-title":"GATA-1-mediated proliferation arrest during erythroid maturation","volume":"23","author":"M Rylski","year":"2003","journal-title":"Mol Cell Biol"},{"issue":"10","key":"pgen.1011617.ref031","doi-asserted-by":"crossref","first-page":"13194","DOI":"10.1096\/fj.202000941R","article-title":"miR-144\/451 inhibits c-Myc to promote erythroid differentiation","volume":"34","author":"L Xu","year":"2020","journal-title":"FASEB Journal"},{"issue":"11","key":"pgen.1011617.ref032","doi-asserted-by":"crossref","first-page":"2492","DOI":"10.1093\/emboj\/19.11.2492","article-title":"ABC-me: a novel mitochondrial transporter induced by GATA-1 during erythroid differentiation","volume":"19","author":"OS Shirihai","year":"2000","journal-title":"EMBO J"},{"issue":"6","key":"pgen.1011617.ref033","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1128\/MCB.00865-13","article-title":"Abcb10 role in heme biosynthesis in vivo: Abcb10 knockout in mice causes anemia with protoporphyrin IX and iron accumulation","volume":"34","author":"M Yamamoto","year":"2014","journal-title":"Mol Cell Biol"},{"issue":"7111","key":"pgen.1011617.ref034","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1038\/nature05125","article-title":"Identification of a mammalian mitochondrial porphyrin transporter","volume":"443","author":"PC Krishnamurthy","year":"2006","journal-title":"Nature"},{"issue":"25","key":"pgen.1011617.ref035","doi-asserted-by":"crossref","first-page":"17083","DOI":"10.1074\/jbc.M801794200","article-title":"Peptide specificity and lipid activation of the lysosomal transport complex ABCB9 (TAPL)","volume":"283","author":"C Zhao","year":"2008","journal-title":"J Biol Chem"},{"key":"pgen.1011617.ref036","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/978-981-15-6082-8_6","article-title":"ABC Transporters, Cholesterol Efflux, and Implications for Cardiovascular Diseases","author":"N Wang","year":"2020","journal-title":"Adv Exp Med Biol"},{"issue":"4","key":"pgen.1011617.ref037","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1016\/j.molcel.2009.11.002","article-title":"Insights into GATA-1-mediated gene activation versus repression via genome-wide chromatin occupancy analysis","volume":"36","author":"M Yu","year":"2009","journal-title":"Mol Cell"},{"issue":"29","key":"pgen.1011617.ref038","doi-asserted-by":"crossref","first-page":"21090","DOI":"10.1074\/jbc.M701228200","article-title":"Sterol regulatory element-binding protein-2- and liver X receptor-driven dual promoter regulation of hepatic ABC transporter A1 gene expression: mechanism underlying the unique response to cellular cholesterol status","volume":"282","author":"N Tamehiro","year":"2007","journal-title":"J Biol Chem"},{"issue":"4","key":"pgen.1011617.ref039","doi-asserted-by":"crossref","first-page":"2306","DOI":"10.1016\/S0021-9258(19)39976-4","article-title":"Identification of nucleotides responsible for enhancer activity of sterol regulatory element in low density lipoprotein receptor gene","volume":"265","author":"JR Smith","year":"1990","journal-title":"J Biol Chem"},{"issue":"21","key":"pgen.1011617.ref040","doi-asserted-by":"crossref","first-page":"3081","DOI":"10.3748\/wjg.v10.i21.3081","article-title":"Maintaining cholesterol homeostasis: sterol regulatory element-binding proteins","volume":"10","author":"L-W Weber","year":"2004","journal-title":"World J Gastroenterol"},{"issue":"2","key":"pgen.1011617.ref041","doi-asserted-by":"crossref","first-page":"e2102669","DOI":"10.1002\/advs.202102669","article-title":"Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation","volume":"9","author":"Z Lu","year":"2022","journal-title":"Adv Sci (Weinh)"},{"issue":"10","key":"pgen.1011617.ref042","doi-asserted-by":"crossref","first-page":"4811","DOI":"10.1210\/en.2005-1647","article-title":"Calpain system regulates the differentiation of adult primitive mesenchymal ST-13 adipocytes","volume":"147","author":"Y Yajima","year":"2006","journal-title":"Endocrinology"},{"issue":"1","key":"pgen.1011617.ref043","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/jcp.1041100109","article-title":"Lipid composition of Friend leukemia cells following induction of erythroid differentiation by dimethyl sulfoxide","volume":"110","author":"LS Rittmann","year":"1982","journal-title":"J Cell Physiol"},{"issue":"3","key":"pgen.1011617.ref044","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1128\/MCB.17.3.1642","article-title":"Erythroid-cell-specific properties of transcription factor GATA-1 revealed by phenotypic rescue of a gene-targeted cell line","volume":"17","author":"M Weiss","year":"1997","journal-title":"Mol Cell Biol"},{"issue":"1","key":"pgen.1011617.ref045","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.bbadis.2010.12.024","article-title":"Mast cell transcription factors--regulators of cell fate and phenotype","author":"S Tshori","year":"2012","journal-title":"Biochim Biophys Acta"},{"issue":"3","key":"pgen.1011617.ref046","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1084\/jem.20070544","article-title":"Antagonism of FOG-1 and GATA factors in fate choice for the mast cell lineage","volume":"205","author":"AB Cantor","year":"2008","journal-title":"J Exp Med"},{"issue":"4","key":"pgen.1011617.ref047","doi-asserted-by":"crossref","first-page":"1924","DOI":"10.1182\/blood-2007-08-104489","article-title":"Differential context-dependent effects of friend of GATA-1 (FOG-1) on mast-cell development and differentiation","volume":"111","author":"D Sugiyama","year":"2008","journal-title":"Blood"},{"issue":"19","key":"pgen.1011617.ref048","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1101\/gad.177200","article-title":"Antagonism between C\/EBPbeta and FOG in eosinophil lineage commitment of multipotent hematopoietic progenitors","volume":"14","author":"E Querfurth","year":"2000","journal-title":"Genes Dev"},{"issue":"4","key":"pgen.1011617.ref049","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1002\/jss.400080404","article-title":"Influence of increased membrane cholesterol on membrane fluidity and cell function in human red blood cells","volume":"8","author":"RA Cooper","year":"1978","journal-title":"J Supramol Struct"},{"issue":"4","key":"pgen.1011617.ref050","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/s41580-019-0190-7","article-title":"Mechanisms and regulation of cholesterol homeostasis","volume":"21","author":"J Luo","year":"2020","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"Suppl","key":"pgen.1011617.ref051","first-page":"S120-5","article-title":"Fifty years of advances in bile acid synthesis and metabolism","author":"DW Russell","year":"2009","journal-title":"J Lipid Res"},{"issue":"2","key":"pgen.1011617.ref052","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.stem.2012.04.024","article-title":"Regulation of hematopoietic stem and progenitor cell mobilization by cholesterol efflux pathways","volume":"11","author":"M Westerterp","year":"2012","journal-title":"Cell Stem Cell"},{"issue":"11","key":"pgen.1011617.ref053","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1161\/ATVBAHA.112.300134","article-title":"Cholesterol efflux: a novel regulator of myelopoiesis and atherogenesis","volume":"32","author":"AR Tall","year":"2012","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"5","key":"pgen.1011617.ref054","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1016\/S0021-9258(19)69789-9","article-title":"Sequential cycles of cholesterol and dolichol synthesis in mouse spleens during phenylhydrazine-induced erythropoiesis","volume":"256","author":"JE Potter","year":"1981","journal-title":"J Biol Chem"},{"issue":"2","key":"pgen.1011617.ref055","first-page":"265","article-title":"Zebrafish ETV7 regulates red blood cell development through the cholesterol synthesis pathway","volume":"7","author":"AM Quintana","year":"2014","journal-title":"Dis Models Mech"},{"issue":"8","key":"pgen.1011617.ref056","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1182\/bloodadvances.2018024539","article-title":"Mutations in the zebrafish hmgcs1 gene reveal a novel function for isoprenoids during red blood cell development","volume":"3","author":"JA Hernandez","year":"2019","journal-title":"Blood Adv"},{"issue":"9","key":"pgen.1011617.ref057","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1194\/jlr.M500511-JLR200","article-title":"The mechanism of ABCG5\/ABCG8 in biliary cholesterol secretion in mice","volume":"47","author":"A Kosters","year":"2006","journal-title":"J Lipid Res"},{"issue":"6","key":"pgen.1011617.ref058","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1161\/ATVBAHA.112.248971","article-title":"Red blood cells play a role in reverse cholesterol transport","volume":"32","author":"KT Hung","year":"2012","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"12","key":"pgen.1011617.ref059","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1515\/hsz-2019-0244","article-title":"Red blood cells participate in reverse cholesterol transport by mediating cholesterol efflux of high-density lipoprotein and apolipoprotein A-I from THP-1 macrophages","volume":"400","author":"S-J Lai","year":"2019","journal-title":"Biol Chem"},{"issue":"5","key":"pgen.1011617.ref060","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1194\/jlr.TR119000267","article-title":"Hematopoiesis is regulated by cholesterol efflux pathways and lipid rafts: connections with cardiovascular diseases","volume":"61","author":"PK Morgan","year":"2020","journal-title":"J Lipid Res"},{"issue":"2","key":"pgen.1011617.ref061","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0167-4889(86)90111-4","article-title":"The inhibition of commitment of mouse erythroleukemia cells by steroids involves a glucocorticoid-receptor mediated process(es) acting at the nuclear level","volume":"889","author":"A Tsiftsoglou","year":"1986","journal-title":"Biochim Biophys Acta"},{"issue":"5","key":"pgen.1011617.ref062","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/j.1365-2184.2011.00771.x","article-title":"Cholesterol synthesis-related enzyme oxidosqualene cyclase is required to maintain self-renewal in primary erythroid progenitors","volume":"44","author":"C Mejia-Pous","year":"2011","journal-title":"Cell Prolif"},{"issue":"12","key":"pgen.1011617.ref063","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1194\/jlr.RA120000635","article-title":"Cholesterol transport between red blood cells and lipoproteins contributes to cholesterol metabolism in blood","volume":"61","author":"R Ohkawa","year":"2020","journal-title":"J Lipid Res"},{"issue":"39","key":"pgen.1011617.ref064","doi-asserted-by":"crossref","first-page":"23464","DOI":"10.1074\/jbc.M115.662668","article-title":"Deficiency in the lipid exporter ABCA1 impairs retrograde sterol movement and disrupts sterol sensing at the endoplasmic reticulum","volume":"290","author":"Y Yamauchi","year":"2015","journal-title":"J Biol Chem"},{"key":"pgen.1011617.ref065","first-page":"421","article-title":"Induction of Erythroid-Specific Expression in Murine Erythroleukemia (MEL) Cell Lines","volume":"7","author":"M Antoniou","year":"1991","journal-title":"Methods Mol Biol"},{"issue":"10","key":"pgen.1011617.ref066","doi-asserted-by":"crossref","first-page":"3136","DOI":"10.1182\/blood-2004-04-1603","article-title":"Global regulation of erythroid gene expression by transcription factor GATA-1","volume":"104","author":"JJ Welch","year":"2004","journal-title":"Blood"},{"issue":"11","key":"pgen.1011617.ref067","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1038\/nprot.2013.143","article-title":"Genome engineering using the CRISPR-Cas9 system","volume":"8","author":"FA Ran","year":"2013","journal-title":"Nat Protoc"},{"issue":"15","key":"pgen.1011617.ref068","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"AM Bolger","year":"2014","journal-title":"Bioinformatics"},{"issue":"4","key":"pgen.1011617.ref069","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon provides fast and bias-aware quantification of transcript expression","volume":"14","author":"R Patro","year":"2017","journal-title":"Nat Methods"},{"issue":"2","key":"pgen.1011617.ref070","doi-asserted-by":"crossref","first-page":"e1007664","DOI":"10.1371\/journal.pcbi.1007664","article-title":"Tximeta: Reference sequence checksums for provenance identification in RNA-seq","volume":"16","author":"MI Love","year":"2020","journal-title":"PLoS Comput Biol"},{"issue":"1","key":"pgen.1011617.ref071","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12864-018-5362-x","article-title":"DEBrowser: interactive differential expression analysis and visualization tool for count data","volume":"20","author":"A Kucukural","year":"2019","journal-title":"BMC Genomics"},{"issue":"5","key":"pgen.1011617.ref072","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"clusterProfiler: an R package for comparing biological themes among gene clusters","volume":"16","author":"G Yu","year":"2012","journal-title":"OMICS"},{"issue":"10","key":"pgen.1011617.ref073","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1101\/gad.7.10.1871","article-title":"Circadian transcription of the cholesterol 7 alpha hydroxylase gene may involve the liver-enriched bZIP protein DBP","volume":"7","author":"DJ Lavery","year":"1993","journal-title":"Genes Dev"},{"key":"pgen.1011617.ref074","first-page":"305","article-title":"Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads","volume":"338","author":"P Rodriguez","year":"2006","journal-title":"Methods Mol Biol"},{"issue":"8","key":"pgen.1011617.ref075","first-page":"e43-9","article-title":"Quantitative analysis of murine terminal erythroid differentiation in vivo: novel method to study normal and disordered erythropoiesis","volume":"121","author":"J Liu","year":"2013","journal-title":"Blood"}],"updated-by":[{"DOI":"10.1371\/journal.pgen.1011617","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000}}],"container-title":["PLOS Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1011617","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T17:46:18Z","timestamp":1742233578000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1011617"}},"subtitle":[],"editor":[{"given":"Lolitika","family":"Mandal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,6]]},"references-count":75,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pgen.1011617","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pgen.1011617","asserted-by":"object"}]},"ISSN":["1553-7404"],"issn-type":[{"value":"1553-7404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,6]]}}}