{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T07:15:35Z","timestamp":1772176535179,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011626","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T00:00:00Z","timestamp":1757980800000}}],"reference-count":95,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T00:00:00Z","timestamp":1757030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Peptidoglycan hydrolases facilitate bacterial cell wall growth by creating space for insertion of new material and allowing physical separation of daughter cells. In\n                    <jats:italic>\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:italic>\n                    , three peptidoglycan amidases, AmiA, AmiB and AmiC, cleave septal peptidoglycan during cell division. The LytM-domain proteins EnvC, NlpD and ActS activate these amidases either from inside the cell or the outer membrane: EnvC binds to the cytoplasmic membrane-anchored divisome components FtsEX, while NlpD and ActS are outer membrane-anchored lipoproteins. Here we report the identification of a novel periplasmic deacetylase called SddA that removes acetyl groups from denuded peptidoglycan glycan strands, the products of amidases. The\n                    <jats:italic>\n                      <jats:italic>sddA<\/jats:italic>\n                    <\/jats:italic>\n                    gene is co-expressed with the gene encoding EnvC, linking SddA function to amidase activation. Consistent with this link, the deletion of\n                    <jats:italic>\n                      <jats:italic>sddA<\/jats:italic>\n                    <\/jats:italic>\n                    alleviates phenotypes associated with lack of amidase activation, while overexpression of\n                    <jats:italic>\n                      <jats:italic>sddA<\/jats:italic>\n                    <\/jats:italic>\n                    alleviates phenotypes related to a defective Tol-Pal system and causes cell chaining due to reduced septum peptidoglycan cleavage. We present a model according to which SddA modulates the activation of the septum-splitting amidases during cell division.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011626","type":"journal-article","created":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T17:44:47Z","timestamp":1757094287000},"page":"e1011626","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["A novel peptidoglycan deacetylase modulates daughter cell separation in E. coli"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2517-5707","authenticated-orcid":true,"given":"V\u00edctor M.","family":"Hern\u00e1ndez-Rocamora","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3996-4141","authenticated-orcid":true,"given":"Alessandra M.","family":"Martorana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7265-2549","authenticated-orcid":true,"given":"Aitana","family":"Belloso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0799-2141","authenticated-orcid":true,"given":"Daniel","family":"Ballesteros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7171-6706","authenticated-orcid":true,"given":"Marta","family":"Zaccaria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6729-7564","authenticated-orcid":true,"given":"Amilcar 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