{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:55:15Z","timestamp":1793318115025,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011632","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000}}],"reference-count":108,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM145317"],"award-info":[{"award-number":["R35GM145317"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI126222"],"award-info":[{"award-number":["R21AI126222"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32GM133353"],"award-info":[{"award-number":["T32GM133353"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Hyphal growth is strongly associated with virulence in the human fungal pathogen\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    . While hyphal transcriptional networks have been the subject of intense study, relatively little is known about post-transcriptional regulation. Previous work reported that P-Body (PB) factors Dhh1 and Edc3 were required for\n                    <jats:italic>C. albicans<\/jats:italic>\n                    virulence and filamentation, suggesting an essential role for post-transcriptional regulation of these processes. However, the molecular roles of these factors have not been determined. To further study the function of PB factors in filamentation, we generated homozygous deletions of\n                    <jats:italic>DHH1<\/jats:italic>\n                    and\n                    <jats:italic>EDC3<\/jats:italic>\n                    in diverse prototrophic clinical strains using transient CRISPR-Cas9. Homozygous\n                    <jats:italic>DHH1<\/jats:italic>\n                    deletion strongly impaired growth, altered filamentation, and exhibited unusual colony morphology in response to heat stress in five strain backgrounds. Using RNA-seq, we found\n                    <jats:italic>DHH1<\/jats:italic>\n                    deletion disrupts the regulation of thousands of genes under both yeast and hyphal growth conditions in SC5314 and P57055. This included upregulation of many stress response genes in the absence of external stress, similar to deletion of the\n                    <jats:italic>S. cerevisiae DHH1<\/jats:italic>\n                    homolog. In contrast, we found\n                    <jats:italic>EDC3<\/jats:italic>\n                    was not required for heat tolerance or filamentation in diverse strains. These results support a model in which\n                    <jats:italic>DHH1<\/jats:italic>\n                    , but not\n                    <jats:italic>EDC3<\/jats:italic>\n                    , represses hyphal stress response transcripts in yeast and remodels the transcriptome during filamentation. Our work supports distinct requirements for specific mRNA decay factors, bolstering evidence for post-transcriptional regulation of filamentation in\n                    <jats:italic>C. albicans<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011632","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T13:35:02Z","timestamp":1742218502000},"page":"e1011632","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Roles of P-body factors in Candida albicans filamentation and stress response"],"prefix":"10.1371","volume":"21","author":[{"given":"Melissa A.","family":"Tosiano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick","family":"Lanni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron P.","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6605-2642","authenticated-orcid":true,"given":"C. Joel","family":"McManus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"pgen.1011632.ref001","doi-asserted-by":"crossref","first-page":"165rv13","DOI":"10.1126\/scitranslmed.3004404","article-title":"Hidden killers: human fungal infections","volume":"4","author":"GD Brown","year":"2012","journal-title":"Sci Transl Med"},{"issue":"20","key":"pgen.1011632.ref002","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1056\/NEJMra1315399","article-title":"Invasive candidiasis","volume":"373","author":"BJ Kullberg","year":"2015","journal-title":"N Engl J Med"},{"issue":"5","key":"pgen.1011632.ref003","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.chom.2013.10.016","article-title":"Candida albicans: adapting to succeed","volume":"14","author":"D Kadosh","year":"2013","journal-title":"Cell Host Microbe"},{"key":"pgen.1011632.ref004","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1242\/jeb.088930","article-title":"Stress adaptation in a pathogenic fungus","volume":"217","author":"A Brown","year":"2014","journal-title":"J Exp Biol"},{"issue":"7597","key":"pgen.1011632.ref005","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1038\/nature17625","article-title":"Candidalysin is a fungal peptide toxin critical for mucosal infection","volume":"532","author":"DL Moyes","year":"2016","journal-title":"Nature"},{"issue":"3","key":"pgen.1011632.ref006","doi-asserted-by":"crossref","first-page":"10.1128\/microbiolspec.MB-0005","DOI":"10.1128\/microbiolspec.MB-0005-2014","article-title":"Candida albicans biofilm development and its genetic control","volume":"3","author":"JV Desai","year":"2015","journal-title":"Microbiol Spectr"},{"issue":"2","key":"pgen.1011632.ref007","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/nrmicro2475","article-title":"Genetic control of Candida albicans biofilm development","volume":"9","author":"JS Finkel","year":"2011","journal-title":"Nat Rev Microbiol"},{"issue":"18","key":"pgen.1011632.ref008","doi-asserted-by":"crossref","first-page":"5385","DOI":"10.1128\/JB.183.18.5385-5394.2001","article-title":"Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance","volume":"183","author":"J Chandra","year":"2001","journal-title":"J Bacteriol"},{"issue":"1","key":"pgen.1011632.ref009","doi-asserted-by":"crossref","first-page":"foaa005","DOI":"10.1093\/femsyr\/foaa005","article-title":"Transcriptional control of hyphal morphogenesis in Candida albicans","volume":"20","author":"S Villa","year":"2020","journal-title":"FEMS Yeast Res"},{"issue":"1","key":"pgen.1011632.ref010","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1080\/21505594.2020.1748930","article-title":"The regulation of hyphae growth in Candida albicans","volume":"11","author":"H Chen","year":"2020","journal-title":"Virulence"},{"issue":"3","key":"pgen.1011632.ref011","doi-asserted-by":"crossref","first-page":"e58613","DOI":"10.1371\/journal.pone.0058613","article-title":"A core filamentation response network in Candida albicans is restricted to eight genes","volume":"8","author":"R Martin","year":"2013","journal-title":"PLoS One"},{"key":"pgen.1011632.ref012","doi-asserted-by":"crossref","first-page":"855229","DOI":"10.3389\/fcimb.2022.855229","article-title":"EFG1, Everyone\u2019s favorite gene in Candida albicans: a comprehensive literature review","volume":"12","author":"VE Glazier","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"pgen.1011632.ref013","unstructured":"Azadmanesh J, Gowen AM, Creger PE, et al. 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