{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T23:58:17Z","timestamp":1778803097681,"version":"3.51.4"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R01AI148788"],"award-info":[{"award-number":["1R01AI148788"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["1R01DE026600"],"award-info":[{"award-number":["1R01DE026600"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2046863"],"award-info":[{"award-number":["2046863"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006928","name":"Ohio State University","doi-asserted-by":"publisher","award":["President\u2019s Postdoctoral Scholars Program Award"],"award-info":[{"award-number":["President\u2019s Postdoctoral Scholars Program Award"]}],"id":[{"id":"10.13039\/100006928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM135119"],"award-info":[{"award-number":["T32GM135119"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    The human fungal pathobiont\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    displays extensive genomic plasticity, including large-scale chromosomal changes such as aneuploidy. Chromosome trisomy appears frequently in natural and laboratory strains of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    . Trisomy of specific chromosomes has been linked to large phenotypic effects, such as increased murine gut colonization by strains trisomic for chromosome 7 (Chr7). However, studies of whole-chromosome aneuploidy are generally limited to the SC5314 genome reference strain, making it unclear whether the imparted phenotypes are conserved across\n                    <jats:italic>C. albicans<\/jats:italic>\n                    genetic backgrounds. Here, we report the presence of a Chr7 trisomy in the \u201dcommensal-like\u201d oral candidiasis strain, 529L, and dissect the contribution of Chr7 trisomy to colonization and virulence in 529L and SC5314. These experiments show that strain background and homolog identity (i.e., AAB vs ABB) interact with Chr7 trisomy to alter commensal and virulence phenotypes in multiple host niches.\n                    <jats:italic>In vitro<\/jats:italic>\n                    filamentation was consistently reduced by Chr7 trisomy in SC5314, but this result was not consistent for 529L. Oral colonization of mice was increased by the presence of a Chr7 trisomy in 529L but not SC5314; conversely, virulence during systemic infection was reduced by Chr7 trisomy in SC5314 but not 529L. Strikingly, the AAB Chr7 trisomy in the SC5314 background rendered this strain avirulent in murine systemic infection. Increased dosage of\n                    <jats:italic>NRG1<\/jats:italic>\n                    failed to reproduce most of the Chr7 trisomy phenotypes. Our results demonstrate that aneuploidy interacts with background genetic variation to produce complex phenotypic patterns that deviate from our current understanding in the genome reference strain.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011650","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T13:30:17Z","timestamp":1751031017000},"page":"e1011650","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Strain background interacts with chromosome 7 aneuploidy to determine commensal and virulence phenotypes in Candida albicans"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8540-5055","authenticated-orcid":true,"given":"Abhishek","family":"Mishra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norma V.","family":"Solis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siobhan M.","family":"Dietz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audra L.","family":"Crouch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott G.","family":"Filler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1683-2170","authenticated-orcid":true,"given":"Matthew Z.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"issue":"5","key":"pgen.1011650.ref001","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3001822","article-title":"Candida albicans selection for human commensalism results in substantial 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Selmecki","year":"2009","journal-title":"PLoS Genet"},{"issue":"2","key":"pgen.1011650.ref008","doi-asserted-by":"crossref","DOI":"10.1093\/genetics\/iyab056","article-title":"The fitness costs and benefits of trisomy of each Candida albicans chromosome","volume":"218","author":"F Yang","year":"2021","journal-title":"Genetics"},{"issue":"5840","key":"pgen.1011650.ref009","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1126\/science.1142210","article-title":"Effects of aneuploidy on cellular physiology and cell division in haploid yeast","volume":"317","author":"EM Torres","year":"2007","journal-title":"Science"},{"issue":"2","key":"pgen.1011650.ref010","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.ccell.2016.12.004","article-title":"Single-chromosome Gains Commonly Function as Tumor Suppressors","volume":"31","author":"JM Sheltzer","year":"2017","journal-title":"Cancer 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