{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T10:32:22Z","timestamp":1767177142115,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011657","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000}}],"reference-count":75,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003042","name":"Else Kr\u00f6ner-Fresenius-Stiftung","doi-asserted-by":"publisher","award":["2011_A200"],"award-info":[{"award-number":["2011_A200"]}],"id":[{"id":"10.13039\/501100003042","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008349","name":"Boehringer Ingelheim","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["2544\/7-1"],"award-info":[{"award-number":["2544\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["LI 2044\/5-1 and LI 2044\/5-2"],"award-info":[{"award-number":["LI 2044\/5-1 and LI 2044\/5-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Baden-W\u00fcrttemberg Stiftung GmbH","award":["ISF-015 CrossIngPanC"],"award-info":[{"award-number":["ISF-015 CrossIngPanC"]}]},{"DOI":"10.13039\/501100013812","name":"Medizinische Fakult\u00e4t, Universit\u00e4t Ulm","doi-asserted-by":"publisher","award":["Bausteinprogram L.SBN.0207"],"award-info":[{"award-number":["Bausteinprogram L.SBN.0207"]}],"id":[{"id":"10.13039\/501100013812","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003042","name":"Else Kr\u00f6ner-Fresenius-Stiftung","doi-asserted-by":"publisher","award":["2022_EKEA.89"],"award-info":[{"award-number":["2022_EKEA.89"]}],"id":[{"id":"10.13039\/501100003042","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001656","name":"Helmholtz-Gemeinschaft","doi-asserted-by":"publisher","award":["iMed - the Helmholtz Initiative on Personalized Medicine"],"award-info":[{"award-number":["iMed - the Helmholtz Initiative on Personalized Medicine"]}],"id":[{"id":"10.13039\/501100001656","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Given their capability to differentiate into each cell type of the human body, human pluripotent stem cells (hPSCs) provide a unique platform for developmental studies. In the current study, we employed this cell system to understand the role of pancreatic progenitor differentiation and proliferation factor (PPDPF), a protein that has been little explored so far. While the zebrafish orthologue\n                    <jats:italic>exdpf<\/jats:italic>\n                    is essential for exocrine pancreas specification, its importance for mammalian and human development has not been studied yet. We implemented a four times CRISPR\/Cas9 nicking approach to knockout\n                    <jats:italic>PPDPF<\/jats:italic>\n                    in human embryonic stem cells (hESCs) and differentiated PPDPF\n                    <jats:sup>KO\/KO<\/jats:sup>\n                    and PPDPF\n                    <jats:sup>WT\/WT<\/jats:sup>\n                    cells towards the pancreatic lineage. In contrast to data obtained from zebrafish, a very modest effect of the knockout was observed in the development of pancreatic progenitors\n                    <jats:italic>in vitro<\/jats:italic>\n                    , not affecting lineage specification upon orthotopic transplantation\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The modest effect is in line with the finding that genetic variants near\n                    <jats:italic>PPDPF<\/jats:italic>\n                    are associated with random glucose levels in humans, but not with type 2 diabetes risk, supporting that dysregulation of this gene may only result in minor alterations of glycaemic balance in humans. In addition, PPDPF is less organ- and cell type specifically expressed in higher vertebrates and its so far reported functions appear highly context-dependent.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011657","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T17:34:24Z","timestamp":1744047264000},"page":"e1011657","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["PPDPF is not a key regulator of human pancreas development"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2817-1974","authenticated-orcid":true,"given":"Markus","family":"Breunig","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0640-0764","authenticated-orcid":true,"given":"Meike","family":"Hohwieler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasmin","family":"Haderspeck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"von Zweydorf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie","family":"Hauff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9051-3526","authenticated-orcid":true,"given":"Lino-Pascal","family":"Pasquini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Wiegreffe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2510-3140","authenticated-orcid":true,"given":"Eleni","family":"Zimmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Medhanie A.","family":"Mulaw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1538-0240","authenticated-orcid":true,"given":"C\u00e9cile","family":"Julier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Simon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian Johannes","family":"Gloeckner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0721-8187","authenticated-orcid":true,"given":"Stefan","family":"Liebau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0592-5232","authenticated-orcid":true,"given":"Alexander","family":"Kleger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"issue":"11","key":"pgen.1011657.ref001","doi-asserted-by":"crossref","first-page":"e293","DOI":"10.1371\/journal.pbio.0060293","article-title":"Exdpf is a key regulator of exocrine pancreas development controlled by retinoic acid and ptf1a in zebrafish","volume":"6","author":"Z Jiang","year":"2008","journal-title":"PLoS Biol"},{"issue":"1","key":"pgen.1011657.ref002","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.bbrc.2019.03.213","article-title":"Circular RNA circ-FOXM1 facilitates cell progression as ceRNA to target PPDPF and MACC1 by sponging miR-1304-5p in non-small cell lung cancer","volume":"513","author":"G Liu","year":"2019","journal-title":"Biochem Biophys Res Commun"},{"issue":"1","key":"pgen.1011657.ref003","doi-asserted-by":"crossref","first-page":"214","DOI":"10.7150\/ijbs.65654","article-title":"PPDPF promotes the progression and acts as an antiapoptotic protein in non-small cell lung cancer","volume":"18","author":"M Yun","year":"2022","journal-title":"Int J Biol Sci"},{"issue":"36","key":"pgen.1011657.ref004","doi-asserted-by":"crossref","first-page":"4244","DOI":"10.1038\/s41388-022-02418-3","article-title":"PPDPF promotes lung adenocarcinoma progression via inhibiting apoptosis and NK cell-mediated cytotoxicity through STAT3","volume":"41","author":"Q-W Zheng","year":"2022","journal-title":"Oncogene"},{"key":"pgen.1011657.ref005","doi-asserted-by":"crossref","first-page":"669603","DOI":"10.3389\/fonc.2021.669603","article-title":"The exocrine differentiation and proliferation factor (EXDPF) gene promotes ovarian cancer tumorigenesis by up-regulating DNA replication pathway","volume":"11","author":"Y Xiao","year":"2021","journal-title":"Front Oncol"},{"issue":"9","key":"pgen.1011657.ref006","doi-asserted-by":"crossref","first-page":"e55060","DOI":"10.15252\/embr.202255060","article-title":"Phosphorylation of PPDPF via IL6-JAK2 activates the Wnt\/\u03b2-catenin pathway in colorectal cancer","volume":"24","author":"Y Zi","year":"2023","journal-title":"EMBO Rep"},{"issue":"2","key":"pgen.1011657.ref007","doi-asserted-by":"crossref","first-page":"e2202448","DOI":"10.1002\/advs.202202448","article-title":"PPDPF Promotes the development of mutant KRAS-driven pancreatic ductal adenocarcinoma by regulating the GEF activity of SOS1","volume":"10","author":"Q-Z Ni","year":"2023","journal-title":"Adv Sci (Weinh)"},{"issue":"4","key":"pgen.1011657.ref008","doi-asserted-by":"crossref","first-page":"112340","DOI":"10.1016\/j.celrep.2023.112340","article-title":"PPDPF suppresses the development of hepatocellular carcinoma through TRIM21-mediated ubiquitination of RIPK1","volume":"42","author":"Y-K Wang","year":"2023","journal-title":"Cell Rep"},{"issue":"9","key":"pgen.1011657.ref009","doi-asserted-by":"crossref","first-page":"e14552","DOI":"10.1097\/MD.0000000000014552","article-title":"Pancreatic progenitor cell differentiation and proliferation factor predicts poor prognosis in heptaocellular carcinoma","volume":"98","author":"Z Mao","year":"2019","journal-title":"Medicine (Baltimore)"},{"key":"pgen.1011657.ref010","doi-asserted-by":"crossref","first-page":"550894","DOI":"10.3389\/fgene.2020.550894","article-title":"Identification of a transcriptomic prognostic signature by machine learning using a combination of small cohorts of prostate cancer","volume":"11","author":"B Vittrant","year":"2020","journal-title":"Front Genet"},{"key":"pgen.1011657.ref011","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1146\/annurev.cellbio.042308.113344","article-title":"Vertebrate endoderm development and organ formation","volume":"25","author":"AM Zorn","year":"2009","journal-title":"Annu Rev Cell Dev Biol"},{"issue":"7502","key":"pgen.1011657.ref012","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/nature13302","article-title":"A draft map of the human proteome","volume":"509","author":"M-S Kim","year":"2014","journal-title":"Nature"},{"issue":"1","key":"pgen.1011657.ref013","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1038\/s42003-021-02818-3","article-title":"Transcriptional changes and the role of ONECUT1 in hPSC pancreatic differentiation","volume":"4","author":"S Heller","year":"2021","journal-title":"Communications Biology"},{"issue":"3","key":"pgen.1011657.ref014","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1136\/gutjnl-2016-312423","article-title":"Human pluripotent stem cell-derived acinar\/ductal organoids generate human pancreas upon orthotopic transplantation and allow disease modelling","volume":"66","author":"M Hohwieler","year":"2017","journal-title":"Gut"},{"issue":"3","key":"pgen.1011657.ref015","doi-asserted-by":"crossref","first-page":"582","DOI":"10.3390\/cells11030582","article-title":"Functional genomic screening in human pluripotent stem cells reveals new roadblocks in early pancreatic endoderm formation","volume":"11","author":"J Kr\u00fcger","year":"2022","journal-title":"Cells"},{"key":"pgen.1011657.ref016","article-title":"Mutations and variants of ONECUT1 in diabetes","author":"A Philippi","year":"2021;27(11):1928-40","journal-title":"Nature Medicine"},{"issue":"4","key":"pgen.1011657.ref017","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.stem.2015.02.013","article-title":"Epigenetic priming of enhancers predicts developmental competence of hESC-derived endodermal lineage intermediates","volume":"16","author":"A Wang","year":"2015","journal-title":"Cell Stem Cell"},{"key":"pgen.1011657.ref018","doi-asserted-by":"crossref","first-page":"e59067","DOI":"10.7554\/eLife.59067","article-title":"Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in development","volume":"10","author":"RJ Geusz","year":"2021","journal-title":"Elife"},{"issue":"1","key":"pgen.1011657.ref019","doi-asserted-by":"crossref","first-page":"6636","DOI":"10.1038\/s41467-021-26950-0","article-title":"Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors","volume":"12","author":"RJ Geusz","year":"2021","journal-title":"Nat Commun"},{"key":"pgen.1011657.ref020","year":"2024"},{"issue":"9","key":"pgen.1011657.ref021","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1038\/s41588-023-01462-3","article-title":"GWAS of random glucose in 476,326 individuals provide insights into diabetes pathophysiology, complications and treatment stratification","volume":"55","author":"V Lagou","year":"2023","journal-title":"Nat Genet"},{"issue":"9","key":"pgen.1011657.ref022","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1038\/s41588-021-00913-z","article-title":"Large-scale cis- and trans-eQTL analyses identify thousands of genetic loci and polygenic scores that regulate blood gene expression","volume":"53","author":"U V\u00f5sa","year":"2021","journal-title":"Nat Genet"},{"issue":"6","key":"pgen.1011657.ref023","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.stem.2021.03.005","article-title":"Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells","volume":"28","author":"M Breunig","year":"2021","journal-title":"Cell Stem Cell"},{"issue":"1","key":"pgen.1011657.ref024","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s13059-016-1012-2","article-title":"Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR","volume":"17","author":"M Haeussler","year":"2016","journal-title":"Genome Biol"},{"issue":"12","key":"pgen.1011657.ref025","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1016\/j.bulcan.2019.08.011","article-title":"Long non-coding RNA H19 and cancer: A competing endogenous RNA","volume":"106","author":"Y Ye","year":"2019","journal-title":"Bull Cancer"},{"issue":"3","key":"pgen.1011657.ref026","doi-asserted-by":"crossref","first-page":"105","DOI":"10.5808\/GI.2014.12.3.105","article-title":"Regulation and function of the peg3 imprinted domain","volume":"12","author":"H He","year":"2014","journal-title":"Genomics Inform"},{"issue":"2","key":"pgen.1011657.ref027","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.stem.2012.11.023","article-title":"Dynamic chromatin remodeling mediated by polycomb proteins orchestrates pancreatic differentiation of human embryonic stem cells","volume":"12","author":"R Xie","year":"2013","journal-title":"Cell Stem Cell"},{"key":"pgen.1011657.ref028","doi-asserted-by":"crossref","first-page":"e15657","DOI":"10.7554\/eLife.15657","article-title":"An integrative transcriptomic atlas of organogenesis in human embryos","volume":"5","author":"DT Gerrard","year":"2016","journal-title":"Elife"},{"issue":"5","key":"pgen.1011657.ref029","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1016\/j.stemcr.2017.09.018","article-title":"Laser capture and deep sequencing reveals the transcriptomic programmes regulating the onset of pancreas and liver differentiation in human embryos","volume":"9","author":"RE Jennings","year":"2017","journal-title":"Stem Cell Reports"},{"issue":"6518","key":"pgen.1011657.ref030","doi-asserted-by":"crossref","first-page":"eaba7721","DOI":"10.1126\/science.aba7721","article-title":"A human cell atlas of fetal gene expression","volume":"370","author":"J Cao","year":"2020","journal-title":"Science"},{"issue":"6","key":"pgen.1011657.ref031","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1128\/MCB.06318-11","article-title":"RNA profiling and chromatin immunoprecipitation-sequencing reveal that PTF1a stabilizes pancreas progenitor identity via the control of MNX1\/HLXB9 and a network of other transcription factors","volume":"32","author":"N Thompson","year":"2012","journal-title":"Mol Cell Biol"},{"issue":"1","key":"pgen.1011657.ref032","doi-asserted-by":"crossref","first-page":"5354","DOI":"10.1038\/s41467-023-40893-8","article-title":"Deciphering early human pancreas development at the single-cell level","volume":"14","author":"Z Ma","year":"2023","journal-title":"Nat Commun"},{"issue":"4","key":"pgen.1011657.ref033","first-page":"346-360.e4","article-title":"A Single-cell transcriptomic map of the human and mouse pancreas reveals inter- and intra-cell population structure","volume":"3","author":"M Baron","year":"2016","journal-title":"Cell Syst"},{"issue":"6","key":"pgen.1011657.ref034","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.7150\/thno.78323","article-title":"Single-cell profiling of GP2-enriched pancreatic progenitors to simultaneously create acinar, ductal, and endocrine organoids","volume":"13","author":"S Merz","year":"2023","journal-title":"Theranostics"},{"issue":"2","key":"pgen.1011657.ref035","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1002\/path.6156","article-title":"DNA methylation-associated allelic inactivation regulates Keratin 19 gene expression during pancreatic development and carcinogenesis","volume":"261","author":"J Kr\u00fcger","year":"2023","journal-title":"J Pathol"},{"issue":"11","key":"pgen.1011657.ref036","doi-asserted-by":"crossref","first-page":"114853","DOI":"10.1016\/j.celrep.2024.114853","article-title":"A ONECUT1 regulatory, non-coding region in pancreatic development and diabetes","volume":"43","author":"S Merz","year":"2024","journal-title":"Cell Rep"},{"issue":"7","key":"pgen.1011657.ref037","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1038\/s41587-022-01219-z","article-title":"Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells","volume":"40","author":"D Balboa","year":"2022","journal-title":"Nature Biotechnology"},{"issue":"19\u201320","key":"pgen.1011657.ref038","doi-asserted-by":"crossref","first-page":"2317","DOI":"10.1007\/s00018-006-6095-6","article-title":"The Foxa family of transcription factors in development and metabolism","volume":"63","author":"JR Friedman","year":"2006","journal-title":"Cell Mol Life Sci"},{"issue":"1","key":"pgen.1011657.ref039","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.ydbio.2020.06.009","article-title":"Foxa1 and Foxa2 orchestrate development of the urethral tube and division of the embryonic cloaca through an autoregulatory loop with Shh","volume":"465","author":"ML Gredler","year":"2020","journal-title":"Developmental Biology"},{"issue":"1","key":"pgen.1011657.ref040","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1053\/j.gastro.2010.04.003","article-title":"Replacement of Rbpj with Rbpjl in the PTF1 complex controls the final maturation of pancreatic acinar cells","volume":"139","author":"T Masui","year":"2010","journal-title":"Gastroenterology"},{"issue":"18","key":"pgen.1011657.ref041","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1242\/dev.120063","article-title":"Human pancreas development","volume":"142","author":"RE Jennings","year":"2015","journal-title":"Development"},{"issue":"17","key":"pgen.1011657.ref042","doi-asserted-by":"crossref","first-page":"5121","DOI":"10.1111\/febs.16075","article-title":"Ptf1a function and transcriptional cis-regulation, a cornerstone in vertebrate pancreas development","volume":"289","author":"M Duque","year":"2022","journal-title":"FEBS J"},{"issue":"1","key":"pgen.1011657.ref043","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0012-1606(03)00308-7","article-title":"Formation of the digestive system in zebrafish. II. Pancreas morphogenesis","volume":"261","author":"HA Field","year":"2003","journal-title":"Dev Biol"},{"issue":"6","key":"pgen.1011657.ref044","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1111\/dgd.12565","article-title":"Zebrafish as a model for studying functional pancreatic \u03b2 cells development and regeneration","volume":"60","author":"H Matsuda","year":"2018","journal-title":"Dev Growth Differ"},{"issue":"2","key":"pgen.1011657.ref045","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.ydbio.2004.07.001","article-title":"Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas","volume":"274","author":"JW Lin","year":"2004","journal-title":"Dev Biol"},{"issue":"9","key":"pgen.1011657.ref046","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1038\/s41588-021-00909-9","article-title":"Mapping the genetic architecture of human traits to cell types in the kidney identifies mechanisms of disease and potential treatments","volume":"53","author":"X Sheng","year":"2021","journal-title":"Nat Genet"},{"issue":"1","key":"pgen.1011657.ref047","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1038\/s41467-021-23285-8","article-title":"PPDPF alleviates hepatic steatosis through inhibition of mTOR signaling","volume":"12","author":"N Ma","year":"2021","journal-title":"Nat Commun"},{"issue":"2","key":"pgen.1011657.ref048","article-title":"USP15 in cancer and other diseases: From diverse functions to therapeutic targets","volume":"10","author":"YC Li","year":"2022","journal-title":"Biomedicines"},{"key":"pgen.1011657.ref049","doi-asserted-by":"crossref","first-page":"603932","DOI":"10.3389\/fonc.2021.603932","article-title":"RBM10 regulates tumor apoptosis, proliferation, and metastasis","volume":"11","author":"Y Cao","year":"2021","journal-title":"Front Oncol"},{"key":"pgen.1011657.ref050","doi-asserted-by":"crossref","first-page":"145463","DOI":"10.1016\/j.gene.2021.145463","article-title":"RBM10: Structure, functions, and associated diseases","volume":"783","author":"A Inoue","year":"2021","journal-title":"Gene"},{"issue":"17","key":"pgen.1011657.ref051","doi-asserted-by":"crossref","first-page":"3481-3495.e7","DOI":"10.1016\/j.molcel.2021.07.019","article-title":"Molecular basis for substrate recruitment to the PRMT5 methylosome","volume":"81","author":"KM Mulvaney","year":"2021","journal-title":"Mol Cell"},{"issue":"3","key":"pgen.1011657.ref052","doi-asserted-by":"crossref","first-page":"58","DOI":"10.36401\/JIPO-22-1","article-title":"Protein arginine methyltransferase 5 (PRMT5) inhibitors in oncology clinical trials: a review","volume":"5","author":"K Feustel","year":"2022","journal-title":"J Immunother Precis Oncol"},{"issue":"13","key":"pgen.1011657.ref053","doi-asserted-by":"crossref","first-page":"8407","DOI":"10.1021\/acs.jmedchem.3c00250","article-title":"Targeting arginine methyltransferase PRMT5 for cancer therapy: updated progress and novel strategies","volume":"66","author":"J Zheng","year":"2023","journal-title":"J Med Chem"},{"issue":"2","key":"pgen.1011657.ref054","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1093\/bib\/bbs017","article-title":"Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration","volume":"14","author":"H Thorvaldsd\u00f3ttir","year":"2012","journal-title":"Brief Bioinform"},{"issue":"1","key":"pgen.1011657.ref055","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s13059-023-03126-1","article-title":"FORGEdb: a tool for identifying candidate functional variants and uncovering target genes and mechanisms for complex diseases","volume":"25","author":"CE Breeze","year":"2024","journal-title":"Genome Biol"},{"issue":"W1","key":"pgen.1011657.ref056","doi-asserted-by":"crossref","first-page":"W191","DOI":"10.1093\/nar\/gkz369","article-title":"g:Profiler: a web server for functional enrichment analysis and conversions of gene lists (2019 update)","volume":"47","author":"U Raudvere","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"pgen.1011657.ref057","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.gde.2015.03.004","article-title":"Islet formation in mice and men: lessons for the generation of functional insulin-producing \u03b2-cells from human pluripotent stem cells","volume":"32","author":"G Nair","year":"2015","journal-title":"Curr Opin Genet Dev"},{"issue":"3","key":"pgen.1011657.ref058","doi-asserted-by":"crossref","first-page":"757","DOI":"10.2337\/db07-1441","article-title":"Reversal of streptozotocin-induced diabetes in mice by cellular transduction with recombinant pancreatic transcription factor pancreatic duodenal homeobox-1: a novel protein transduction domain-based therapy","volume":"57","author":"V Koya","year":"2008","journal-title":"Diabetes"},{"issue":"6","key":"pgen.1011657.ref059","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1007\/BF00267823","article-title":"A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes","volume":"100","author":"N Schaeren-Wiemers","year":"1993","journal-title":"Histochemistry"},{"issue":"7","key":"pgen.1011657.ref060","doi-asserted-by":"crossref","first-page":"e41958","DOI":"10.1371\/journal.pone.0041958","article-title":"Small molecule-assisted, line-independent maintenance of human pluripotent stem cells in defined conditions","volume":"7","author":"S Frank","year":"2012","journal-title":"PLoS One"},{"issue":"1","key":"pgen.1011657.ref061","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/s41467-017-00561-0","article-title":"Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors","volume":"8","author":"KF Cogger","year":"2017","journal-title":"Nat Commun"},{"issue":"4","key":"pgen.1011657.ref062","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1016\/j.stemcr.2022.03.004","article-title":"GP2-enriched pancreatic progenitors give rise to functional beta cells in vivo and eliminate the risk of teratoma formation","volume":"17","author":"Y Aghazadeh","year":"2022","journal-title":"Stem Cell Reports"},{"key":"pgen.1011657.ref063","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.scr.2017.03.015","article-title":"Generation and cardiac subtype-specific differentiation of PITX2-deficient human iPS cell lines for exploring familial atrial fibrillation","volume":"21","author":"M Marczenke","year":"2017","journal-title":"Stem Cell Res"},{"issue":"6121","key":"pgen.1011657.ref064","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex genome engineering using CRISPR\/Cas systems","volume":"339","author":"L Cong","year":"2013","journal-title":"Science"},{"key":"pgen.1011657.ref065","doi-asserted-by":"crossref","first-page":"170185","DOI":"10.1038\/sdata.2017.185","article-title":"An RNA-Seq atlas of gene expression in mouse and rat normal tissues","volume":"4","author":"JF S\u00f6llner","year":"2017","journal-title":"Sci Data"},{"issue":"1","key":"pgen.1011657.ref066","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"A Dobin","year":"2013","journal-title":"Bioinformatics"},{"issue":"11","key":"pgen.1011657.ref067","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/bioinformatics\/bts196","article-title":"RNA-SeQC: RNA-seq metrics for quality control and process optimization","volume":"28","author":"DS DeLuca","year":"2012","journal-title":"Bioinformatics"},{"issue":"1","key":"pgen.1011657.ref068","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1186\/s12859-016-1276-2","article-title":"dupRadar: a Bioconductor package for the assessment of PCR artifacts in RNA-Seq data","volume":"17","author":"S Sayols","year":"2016","journal-title":"BMC Bioinformatics"},{"issue":"7","key":"pgen.1011657.ref069","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Y Liao","year":"2014","journal-title":"Bioinformatics"},{"issue":"1","key":"pgen.1011657.ref070","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/nbt.2450","article-title":"Differential analysis of gene regulation at transcript resolution with RNA-seq","volume":"31","author":"C Trapnell","year":"2013","journal-title":"Nat Biotechnol"},{"issue":"12","key":"pgen.1011657.ref071","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"MI Love","year":"2014","journal-title":"Genome Biol"},{"issue":"43","key":"pgen.1011657.ref072","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"A Subramanian","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"pgen.1011657.ref073","doi-asserted-by":"crossref","first-page":"3144","DOI":"10.1038\/s41467-021-23295-6","article-title":"A 3D system to model human pancreas development and its reference single-cell transcriptome atlas identify signaling pathways required for progenitor expansion","volume":"12","author":"CA Gon\u00e7alves","year":"2021","journal-title":"Nat Commun"},{"issue":"2","key":"pgen.1011657.ref074","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/s41587-023-01767-y","article-title":"Dictionary learning for integrative, multimodal and scalable single-cell analysis","volume":"42","author":"Y Hao","year":"2024","journal-title":"Nature Biotechnology"},{"issue":"16","key":"pgen.1011657.ref075","doi-asserted-by":"crossref","first-page":"3166","DOI":"10.1128\/MCB.00364-13","article-title":"Program specificity for Ptf1a in pancreas versus neural tube development correlates with distinct collaborating cofactors and chromatin accessibility","volume":"33","author":"DM Meredith","year":"2013","journal-title":"Mol Cell Biol"}],"updated-by":[{"DOI":"10.1371\/journal.pgen.1011657","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000}}],"container-title":["PLOS Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1011657","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T13:57:41Z","timestamp":1745848661000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pgen.1011657"}},"subtitle":[],"editor":[{"given":"Fengwei","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,4,7]]},"references-count":75,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pgen.1011657","relation":{},"ISSN":["1553-7404"],"issn-type":[{"value":"1553-7404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,7]]}}}