{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T10:59:04Z","timestamp":1767178744025,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011792","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000}}],"reference-count":70,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM145352"],"award-info":[{"award-number":["R35GM145352"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R03AI178064"],"award-info":[{"award-number":["R03AI178064"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    The self-renewal and differentiation of germline stem cells (GSCs) are tightly regulated during oogenesis. The\n                    <jats:italic>Drosophila<\/jats:italic>\n                    female germline provides a powerful model to study these regulatory mechanisms. We previously identified Sakura (also known as Bourbon\/CG14545) as a crucial factor for maintenance and differentiation of GSCs and oogenesis, and demonstrated that Sakura binds to Ovarian Tumor (Otu), another essential regulator of these processes. Here, we identify MYCBP (c-Myc binding protein) as an additional essential component of this regulatory network. We show that MYCBP physically associates with itself, Sakura, and Otu, forming binary and ternary complexes including a MYCBP\u2022Sakura\u2022Otu complex. MYCBP is highly expressed in the ovary, and\n                    <jats:italic>mycbp<\/jats:italic>\n                    null mutant females exhibit rudimentary ovaries with germline-less and tumorous ovarioles, fail to produce eggs, and are completely sterile. Germline-specific depletion of\n                    <jats:italic>mycbp<\/jats:italic>\n                    disrupts Dpp\/BMP signaling, causing aberrant expression of\n                    <jats:italic>bag-of-marbles<\/jats:italic>\n                    (\n                    <jats:italic>bam<\/jats:italic>\n                    ) and leading to defective differentiation and GSC loss. In addition,\n                    <jats:italic>mycbp<\/jats:italic>\n                    is required for female-specific splicing of\n                    <jats:italic>sex-lethal<\/jats:italic>\n                    (\n                    <jats:italic>sxl<\/jats:italic>\n                    ), a master regulator of sex identity determination. These phenotypes closely resemble those observed in\n                    <jats:italic>sakura<\/jats:italic>\n                    and\n                    <jats:italic>otu<\/jats:italic>\n                    mutants. Together, our findings reveal that MYCBP functions in concert with Sakura and Otu to coordinate self-renewal and differentiation of GSCs and oogenesis in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011792","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T18:59:58Z","timestamp":1764615598000},"page":"e1011792","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["MYCBP interacts with Sakura and Otu and is essential for germline stem cell renewal and differentiation and oogenesis"],"prefix":"10.1371","volume":"21","author":[{"given":"Azali","family":"Azlan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5814-8206","authenticated-orcid":true,"given":"Ryuya","family":"Fukunaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,12,1]]},"reference":[{"issue":"1","key":"pgen.1011792.ref001","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1387\/ijdb.8735924","article-title":"Drosophila differentiation genes instrumental in tumor suppression","volume":"40","author":"E Gateff","year":"1996","journal-title":"Int J Dev Biol"},{"key":"pgen.1011792.ref002","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1146\/annurev.genet.31.1.455","article-title":"The tao of stem cells in the germline","volume":"31","author":"H Lin","year":"1997","journal-title":"Annu Rev 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