{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:56:30Z","timestamp":1787021790801,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011809","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000}}],"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00c3\u00b3n","doi-asserted-by":"publisher","award":["PID2021-125480 NB-I00"],"award-info":[{"award-number":["PID2021-125480 NB-I00"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00c3\u00b3n","doi-asserted-by":"publisher","award":["MDM-2016-0687"],"award-info":[{"award-number":["MDM-2016-0687"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00c3\u00b3n","doi-asserted-by":"publisher","award":["CEX2020-001088-M"],"award-info":[{"award-number":["CEX2020-001088-M"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00c3a","doi-asserted-by":"publisher","award":["P20_00888"],"award-info":[{"award-number":["P20_00888"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Core funding to the CABD from Junta de Andaluc\u00c3a"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Proper gonad development is a pre-requisite for gametogenesis and reproduction. During female gonad formation in\n                    <jats:italic>\n                      <jats:italic>Drosophila<\/jats:italic>\n                    <\/jats:italic>\n                    , the EGF receptor (EGFR) signalling pathway ensures the correct number of primordial germ cells (PGCs) populate the larval gonad. We study the gene\n                    <jats:italic>\n                      <jats:italic>pointed (pnt)<\/jats:italic>\n                    <\/jats:italic>\n                    , which acts downstream of the EGFR receptor and belongs to the ETS transcription factor family, with a previously unknown function in gonadogenesis. We report that\n                    <jats:italic>\n                      <jats:italic>pnt<\/jats:italic>\n                    <\/jats:italic>\n                    is expressed in female larval gonads and later in the adult ovarian germline niche and that it is required to sustain proper gametogenesis. Loss of\n                    <jats:italic>\n                      <jats:italic>pnt<\/jats:italic>\n                    <\/jats:italic>\n                    function in female larval gonads, similar to the EGFR, induced PGC overproliferation. Conversely, we isolated a novel mutant allele gene, termed\n                    <jats:italic>\n                      <jats:italic>pnt<\/jats:italic>\n                    <\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>aga<\/jats:italic>\n                    <\/jats:sup>\n                    , which resulted in agametic gonads and ovaries. While\n                    <jats:italic>\n                      <jats:italic>pnt<\/jats:italic>\n                    <\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>aga<\/jats:italic>\n                    <\/jats:sup>\n                    embryos developed gonads containing a normal complement of PGCs, these are subsequently lost by apoptosis during late larval and pupal stages. Molecular characterization of\n                    <jats:italic>\n                      <jats:italic>pnt<\/jats:italic>\n                    <\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>aga<\/jats:italic>\n                    <\/jats:sup>\n                    revealed reduced expression levels of the different\n                    <jats:italic>\n                      <jats:italic>pnt<\/jats:italic>\n                    <\/jats:italic>\n                    isoforms, unveiling a complex autoregulatory network involving the three Pnt proteins. We propose that germline survival in\n                    <jats:italic>\n                      <jats:italic>Drosophila<\/jats:italic>\n                    <\/jats:italic>\n                    gonads requires a precise tuning of EGFR signalling to ensure the appropriate transcriptional activation of its target\n                    <jats:italic>\n                      <jats:italic>pnt<\/jats:italic>\n                    <\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011809","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T17:35:02Z","timestamp":1756143302000},"page":"e1011809","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["ETS transcription factor pointed controls germline survival in Drosophila"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9119-1604","authenticated-orcid":true,"given":"Alicia E.","family":"Rosales-Nieves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Mar\u00edn-Menguiano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5509-3804","authenticated-orcid":true,"given":"Lourdes","family":"L\u00f3pez-Onieva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4534-4109","authenticated-orcid":true,"given":"Juan","family":"Garrido-Maraver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3048-0968","authenticated-orcid":true,"given":"Acaimo","family":"Gonz\u00e1lez-Reyes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"key":"pgen.1011809.ref001","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbcan.2023.188872","article-title":"ETS transcription factors: multifaceted players from cancer progression to tumor immunity","volume":"1878","author":"Y Wang","year":"2023","journal-title":"Biochimica et Biophysica Acta - Reviews on Cancer"},{"issue":"2","key":"pgen.1011809.ref002","article-title":"Ets transcription factors and human disease","volume":"1377","author":"J Dittmer","year":"1998","journal-title":"Biochim Biophys Acta"},{"issue":"11","key":"pgen.1011809.ref003","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/35099076","article-title":"The ETS-domain transcription factor family","volume":"2","author":"AD Sharrocks","year":"2001","journal-title":"Nat Rev Mol Cell Biol"},{"key":"pgen.1011809.ref004","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1002\/bies.10068","article-title":"When Ets transcription factors meet their partners","volume":"24","author":"A Verger","year":"2002","journal-title":"BioEssays"},{"key":"pgen.1011809.ref005","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0378-1119(02)01156-3","article-title":"Molecular biology of the Ets family of transcription factors","volume":"303","author":"T Oikawa","year":"2003","journal-title":"Gene"},{"issue":"55","key":"pgen.1011809.ref006","doi-asserted-by":"crossref","first-page":"6409","DOI":"10.1038\/sj.onc.1204033","article-title":"Sequence and functional properties of Ets genes in the model organism Drosophila","volume":"19","author":"T Hsu","year":"2000","journal-title":"Oncogene"},{"issue":"9","key":"pgen.1011809.ref007","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1101\/gad.4.9.1451","article-title":"The ETS-domain: a new DNA-binding motif that recognizes a purine-rich core DNA sequence","volume":"4","author":"FD Karim","year":"1990","journal-title":"Genes Dev"},{"issue":"1","key":"pgen.1011809.ref008","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1242\/dev.117.1.163","article-title":"The Drosophila gene pointed encodes two ETS-like proteins which are involved in the development of the midline glial cells","volume":"117","author":"C Kl\u00e4mbt","year":"1993","journal-title":"Development"},{"issue":"1","key":"pgen.1011809.ref009","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0092-8674(94)90580-0","article-title":"The activities of two Ets-related transcription factors required for Drosophila eye development are modulated by the Ras\/MAPK pathway","volume":"78","author":"EM O\u2019Neill","year":"1994","journal-title":"Cell"},{"issue":"12","key":"pgen.1011809.ref010","doi-asserted-by":"crossref","first-page":"3745","DOI":"10.1242\/dev.122.12.3745","article-title":"Pointed, an ETS domain transcription factor, negatively regulates the EGF receptor pathway in Drosophila oogenesis","volume":"122","author":"AM Morimoto","year":"1996","journal-title":"Development"},{"key":"pgen.1011809.ref011","doi-asserted-by":"crossref","unstructured":"Scholz H, Deatrick J, Klaes A, Klambt C. 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