{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:40:00Z","timestamp":1781541600381,"version":"3.54.5"},"reference-count":81,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS-155790"],"award-info":[{"award-number":["IOS-155790"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Health","award":["GM072562"],"award-info":[{"award-number":["GM072562"]}]},{"name":"National Institute of Health","award":["CA224381"],"award-info":[{"award-number":["CA224381"]}]},{"name":"National Institute of Health","award":["CA227789"],"award-info":[{"award-number":["CA227789"]}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    The differentiation of epithelial cells into Non-Professional Phagocytes (NPPs) is essential for maintaining tissue homeostasis and clearing apoptotic debris. In the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    ovary, epithelial follicle cells transform into NPPs following germline cell death, but the genetic mechanisms controlling this transition are not well defined. To investigate these mechanisms, we used a model in which overexpression of the active form of Notch, the Notch Intracellular Domain (NICD), induces a robust epithelial-to-NPP transition. Using single-cell RNA sequencing and trajectory analysis, we identified three transcriptional phases of NPP maturation: an early stage of metabolic activation, an intermediate stage enriched in genes related to migration and cytoskeletal remodeling, and a late stage marked by autophagy-related gene expression. These transcriptomic patterns were validated by immunostaining. SCENIC and ChiP-seq analyses identified the JNK effector Jun-related antigen (Jra) and its predicted targets,\n                    <jats:italic>Arp2<\/jats:italic>\n                    and\n                    <jats:italic>Arp3<\/jats:italic>\n                    , which encode components of the Arp2\/3 complex, as regulators of cytoskeletal remodeling. Functional assays confirmed that the JNK\u2013Jra\u2013Arp2\/3 axis is required for cytoplasmic expansion and debris clearance during NPP differentiation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011953","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T18:33:56Z","timestamp":1764873236000},"page":"e1011953","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Single-cell transcriptomics reveals stepwise transformation of epithelial cells into Non-Professional Phagocytes"],"prefix":"10.1371","volume":"21","author":[{"given":"Caique","family":"Almeida Machado Costa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0848-8169","authenticated-orcid":true,"given":"Cristian Alejandro","family":"Santiago-Santiago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Chun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9098-9769","authenticated-orcid":true,"given":"Wu-Min","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"issue":"9","key":"pgen.1011953.ref001","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.3390\/cells10092222","article-title":"Modeling Notch-Induced Tumor Cell Survival in the Drosophila Ovary Identifies Cellular and Transcriptional Response to Nuclear NICD Accumulation","volume":"10","author":"A 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