{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T08:18:44Z","timestamp":1767773924444,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011980","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000}}],"reference-count":70,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GO2495\/9-1"],"award-info":[{"award-number":["GO2495\/9-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    The distinct textural properties of fruits in varying stages of ripening present unique ecological opportunities for several species of fruit flies, resulting, over evolutionary times, in specialized egg-laying behaviors. In this study we identified a TrpA channel-dependent mechanosensory pathway in the legs, through the gene\n                    <jats:italic>painless,<\/jats:italic>\n                    that modulates the discernment of softer patches for oviposition in gravid\n                    <jats:italic>D. melanogaster<\/jats:italic>\n                    females. We report that the stiffness-sensing role of tarsi is mediated through external sensory organs, namely ventral mechanosensory bristles and subsets of campaniform sensilla present primarily at the joints between tarsomeres. Our findings provide new evidence that campaniform sensilla function as indirect stiffness sensors of oviposition substrates, owing to their placement at joints that experience maximal cuticular distortion. We show that Painless is expressed in mechanosensory neurons innervating peripheral organs and is necessary for their functions in mediating oviposition substrate selection in gravid females. Furthermore, we observed that overexpression of\n                    <jats:italic>painless<\/jats:italic>\n                    in both campaniform sensilla and mechanosensory bristles partially rescues preference for the softer substrates in\n                    <jats:italic>painless<\/jats:italic>\n                    mutants, indicating that\n                    <jats:italic>painless<\/jats:italic>\n                    activity in these organs is necessary to mediate the preference. We propose that different interactions with a soft\n                    <jats:italic>vs.<\/jats:italic>\n                    a hard substrate (compression of the cuticle, distribution of contacts) results in differential mechanotransduction in\n                    <jats:italic>painless<\/jats:italic>\n                    -expressing neurons, determining oviposition preferences.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011980","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T18:50:31Z","timestamp":1767034231000},"page":"e1011980","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The TRP-channel painless mediates substrate stiffness sensing in the legs during Drosophila oviposition"],"prefix":"10.1371","volume":"21","author":[{"given":"Vijayaditya","family":"Ray","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lasse B.","family":"Br\u00e4cker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandros","family":"Kourtidis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3469-7051","authenticated-orcid":true,"given":"Charlie","family":"Rosher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gesa F.","family":"Dinges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Pierzchli\u0144ska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ansgar","family":"B\u00fcschges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin M.","family":"Cury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3358-7963","authenticated-orcid":true,"given":"Nicolas","family":"Gompel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,12,29]]},"reference":[{"issue":"24","key":"pgen.1011980.ref001","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.1016\/j.cub.2013.10.047","article-title":"Olfactory preference for egg laying on citrus substrates in Drosophila","volume":"23","author":"HKM Dweck","year":"2013","journal-title":"Curr Biol"},{"issue":"5870","key":"pgen.1011980.ref002","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1126\/science.1151842","article-title":"Drosophila egg-laying site selection as a system to study simple decision-making processes","volume":"319","author":"C-H Yang","year":"2008","journal-title":"Science"},{"issue":"6","key":"pgen.1011980.ref003","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1016\/j.celrep.2018.07.018","article-title":"Olfactory neurons and brain centers directing oviposition decisions in Drosophila","volume":"24","author":"SG Chin","year":"2018","journal-title":"Cell Rep"},{"issue":"2","key":"pgen.1011980.ref004","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.celrep.2014.09.033","article-title":"Mechanosensitive neurons on the internal reproductive tract contribute to egg-laying-induced acetic acid attraction in Drosophila","volume":"9","author":"B Gou","year":"2014","journal-title":"Cell Rep"},{"issue":"1","key":"pgen.1011980.ref005","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/BF01067322","article-title":"Behavior genetics of choice of oviposition sites in Drosophila melanogaster. 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