{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T18:35:36Z","timestamp":1786732536160,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011985","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000}}],"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006734","name":"Princeton University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006734","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100024037","name":"Princeton Neuroscience Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100024037","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Princeton University, Center for Health and Wellbeing"}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Previous research suggests that social isolation induces changes in gene expression that encode a starvation-like brain state and reduce sleep. However, the extent to which social isolation alters behaviors via sex-specific brain changes is unclear. Here, we use\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    to explore sex differences in isolation-induced behavioral and transcriptomic changes. Male and female adult flies were isolated for seven days, and multiple behavioral sex-based differences were identified through tests of activity, motivation, aggression, and sugar preference. RNA-seq analysis also identified several candidate genes that were associated with sex differences in isolation-induced behavioral changes.\n                    <jats:italic>Takeout<\/jats:italic>\n                    (\n                    <jats:italic>to<\/jats:italic>\n                    ) expression and sucrose selection were upregulated exclusively in females following social isolation. Following\n                    <jats:italic>to<\/jats:italic>\n                    knockdown in\n                    <jats:italic>to<\/jats:italic>\n                    -expressing cells, sucrose preference decreased in socially isolated females but increased in males. Overall, our results suggest that manipulating\n                    <jats:italic>to<\/jats:italic>\n                    expression influences sucrose choice in opposite directions between females and males following social isolation. It is possible that isolation-induced\n                    <jats:italic>to<\/jats:italic>\n                    overexpression in non-neuronal cells in the female head could contribute to sex differences in this behavior.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011985","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T17:39:49Z","timestamp":1786383589000},"page":"e1011985","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Social isolation upregulates takeout expression in female Drosophila melanogaster to promote sucrose preference"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9226-4450","authenticated-orcid":true,"given":"Elisabeth A.","family":"Tawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1365-1000","authenticated-orcid":true,"given":"Lisa","family":"Schneper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine I.","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2475-4200","authenticated-orcid":true,"given":"Mindy","family":"Sim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9409-8739","authenticated-orcid":true,"given":"Eve","family":"Schoeffler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8809-8297","authenticated-orcid":true,"given":"Daniel A.","family":"Notterman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"pgen.1011985.ref001","unstructured":"WHO Commission on Social Connection. 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