{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T18:05:27Z","timestamp":1784570727519,"version":"3.55.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1011986","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000}}],"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Independent Research Fund Denmark","award":["1026-00006B"],"award-info":[{"award-number":["1026-00006B"]}]},{"DOI":"10.13039\/501100006356","name":"University of Southern Denmark","doi-asserted-by":"crossref","award":["Start-up funding"],"award-info":[{"award-number":["Start-up funding"]}],"id":[{"id":"10.13039\/501100006356","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    The DNA damage (SOS) response in bacteria involves derepression of a set of genes in order to activate mechanisms to tolerate stress, repair DNA and slow down cell division. While some of these genes are well characterized, many genes exist which are clearly induced by DNA damage but for which the function is unclear. In\n                    <jats:italic>Caulobacter crescentus<\/jats:italic>\n                    , the toxin-antitoxin (TA) system\n                    <jats:italic>higBA<\/jats:italic>\n                    and a closely associated downstream transcription factor (\n                    <jats:italic>higX<\/jats:italic>\n                    ) are strongly induced as part of the SOS response, but the role of\n                    <jats:italic>higX<\/jats:italic>\n                    is unknown. We show that, unexpectedly, HigX functions independently of HigBA as a cell membrane-associated regulator and is toxic when overexpressed in filamentous cells. ChIP-Seq indicated that it binds to several promoters associated with cell envelope regulation. In cells with the SOS response constitutively activated, HigX was overproduced, but at the same time was unable to bind the majority of its target promoters.\n                    <jats:italic>higX<\/jats:italic>\n                    was conserved among many alpha-proteobacteria, while\n                    <jats:italic>higBA<\/jats:italic>\n                    was only found upstream of it in a small number of\n                    <jats:italic>Caulobacter<\/jats:italic>\n                    genomes, including the universal laboratory strain\n                    <jats:italic>C. crescentus<\/jats:italic>\n                    NA1000. Compositional analysis suggested that\n                    <jats:italic>higBA<\/jats:italic>\n                    originated from a foreign source, while\n                    <jats:italic>higX<\/jats:italic>\n                    is likely ancestral to the alpha-proteobacteria. Our data support a model where dysregulation of HigX production and activity in filamentous cells contributes to cell envelope instability and antibiotic sensitivity. Thus, the protective effect of inhibiting cell division during the SOS response in order to repair the DNA, carries the hidden cost of interference with HigX-mediated cell envelope maintenance.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1011986","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T17:45:57Z","timestamp":1784137557000},"page":"e1011986","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["An ancestral transmembrane transcription factor couples cell envelope regulation and the SOS response in Caulobacter crescentus"],"prefix":"10.1371","volume":"22","author":[{"given":"Kamilla Ank\u00e6r","family":"Brejndal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaj 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