{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T20:47:31Z","timestamp":1770756451755,"version":"3.50.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1012033","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000}}],"reference-count":110,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008109","name":"University of Notre Dame","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008109","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Elizabeth and Michael Gallagher Family"},{"name":"Fitzgerald Family"},{"name":"the Centers for Zebrafish Research and Stem Cells and Regenerative Medicine at the University of Notre Dame"},{"name":"Indiana Spinal Cord and Brain Injury Research Trust through the Indiana State Board of Health"},{"name":"SMART Foundation"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DP2NS117177"],"award-info":[{"award-number":["DP2NS117177"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Tissues and organs have periods of plasticity that close with age. While period closures can lock in tissue architecture and prevent aberrant cellular interactions, they also limit regenerative capacity. These regenerative periods \u2013 a timeframe with regeneration capacity \u2013 are defined, but the underlying genetic mechanisms that close specific regenerative periods remains critical knowledge that needs expanding. Here, we established zebrafish larvae as a model to study the genetic basis of regenerative period closure. We demonstrated that laser axotomy of the centrally-projecting axons of dorsal root ganglia (DRG) neurons exhibit a robust regenerative period that is closed by 3 days post fertilization (dpf). The closure of the regenerative period corresponds with the rearrangement of glia that express\n                    <jats:italic>netrin<\/jats:italic>\n                    , introducing the idea that changes in the DCC-mediated signaling axis could be a genetic and molecular basis closing the regenerative period. To test this hypothesis, we manipulated\n                    <jats:italic>dcc<\/jats:italic>\n                    , cAMP, and Rac1 in transgenic animals that label axons and the actin cytoskeleton. Combined with genetic epistasis analysis, we show that altering DCC signaling can re-open the regenerative period, allowing severed axons to regrow into the spinal cord. We show that this increased capacity to reinvade the spinal cord is mediated by growth cone invadopodia. Using calcium reporters and behavioral analysis, we demonstrate that re-opening the regenerative period by manipulating the DCC signaling axis restores the sensory circuit and sensory-specific behaviors. By introducing this genetic basis for regenerative period closure, these results reveal an active suppression process that keeps regenerative periods closed and establishes a new model for future dissection of such periods.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1012033","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T18:54:36Z","timestamp":1770058476000},"page":"e1012033","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The regenerative period of somatosensory nerves is closed by a DCC signaling axis"],"prefix":"10.1371","volume":"22","author":[{"given":"Jacob","family":"Hammer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9831-1514","authenticated-orcid":true,"given":"Cody J.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"issue":"12","key":"pgen.1012033.ref001","article-title":"Decoding aging-dependent regenerative decline across tissues at single-cell resolution","volume":"30","author":"Y Cai","year":"2023","journal-title":"Cell Stem Cell"},{"key":"pgen.1012033.ref002","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1101\/gad.351728.124","article-title":"A cellular identity crisis? 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