{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T01:21:00Z","timestamp":1789348860684,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.pgen.1012106","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000}}],"reference-count":133,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009053","name":"The Wellcome Trust DBT India Alliance","doi-asserted-by":"publisher","award":["IA\/S\/22\/1\/506243"],"award-info":[{"award-number":["IA\/S\/22\/1\/506243"]}],"id":[{"id":"10.13039\/501100009053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001502","name":"Department of Atomic Energy","doi-asserted-by":"crossref","award":["1303\/2\/2019\/R&D-II\/DAE\/2079"],"award-info":[{"award-number":["1303\/2\/2019\/R&D-II\/DAE\/2079"]}],"id":[{"id":"10.13039\/501100001502","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    The polarized architecture of neurons is intricately associated with the modulation of microtubule dynamics. Over the years, several microtubule-associated factors that regulate neuronal polarity have been identified. However, the precise details of how microtubule arrangement and stability are established in axons and dendrites are not clearly understood. To uncover the relevant factors involved in the biological pathways governing microtubule regulation in neurons, we conducted a suppressor screen using the neuronal ectopic extension phenotype caused by the loss of the kinesin-13 family microtubule depolymerizing protein KLP-7 in\n                    <jats:italic>C. elegans<\/jats:italic>\n                    . Interestingly, apart from eleven variants of \u03b1 (\n                    <jats:italic>mec-12<\/jats:italic>\n                    ) and \u03b2 (\n                    <jats:italic>mec-7<\/jats:italic>\n                    ) tubulins, we isolated a variant of cytokinesis-associated protein,\n                    <jats:italic>W02B8.2\/citk-1,<\/jats:italic>\n                    the suggested kinase-less orthologue of mammalian citron-rho interacting kinase (CIT). Little is known about the role of CIT in microtubule regulation in post-mitotic neurons. In this study, we found that the kinase-less worm orthologues of CIT,\n                    <jats:italic>citk-1<\/jats:italic>\n                    and\n                    <jats:italic>citk-2,<\/jats:italic>\n                    redundantly modulate microtubule stability in the axon-like anterior process and maintain the population of plus-end-out microtubules in the dendrite-like posterior process of the PLM mechanosensory neurons in a cell-autonomous manner. In the absence of\n                    <jats:italic>citk-1<\/jats:italic>\n                    and\n                    <jats:italic>citk-2<\/jats:italic>\n                    , PLM neurons exhibit variable morphological defects, including neurite growth and synaptic branch defects. Moreover, we find that CITK-1\/2 work in the same genetic pathway as ASPM-1 (the worm homolog of mammalian ASPM (abnormal spindle-like microcephaly-associated protein)) to modulate plus-end dynamics of microtubules in PLM neurons. Our findings suggest that the cytokinesis-associated CITK-1\/2 and ASPM-1 have non-mitotic roles in regulation of microtubules in differentiated PLM neurons.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1012106","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T18:00:26Z","timestamp":1776276026000},"page":"e1012106","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Worm orthologues of cytokinesis-associated proteins CIT and ASPM regulate neuronal microtubule dynamics and polarity in C. elegans"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0870-3750","authenticated-orcid":true,"given":"Sunanda","family":"Sharma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4066-5002","authenticated-orcid":true,"given":"Keerthana","family":"Ponniah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4744-860X","authenticated-orcid":true,"given":"Ishanee","family":"Bandyopadhyay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0761-0795","authenticated-orcid":true,"given":"Devyani","family":"Vadawale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandhya P.","family":"Koushika","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3428-4588","authenticated-orcid":true,"given":"Anindya","family":"Ghosh-Roy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,4,15]]},"reference":[{"issue":"8","key":"pgen.1012106.ref001","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1038\/nrn2852","article-title":"The axon initial segment and the maintenance of neuronal polarity","volume":"11","author":"MN Rasband","year":"2010","journal-title":"Nat Rev Neurosci"},{"issue":"9","key":"pgen.1012106.ref002","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.202005080","article-title":"Wnt signaling establishes the microtubule polarity in neurons through regulation of Kinesin-13","volume":"220","author":"D Puri","year":"2021","journal-title":"J Cell Biol"},{"issue":"3","key":"pgen.1012106.ref003","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1083\/jcb.200707042","article-title":"Microtubule stabilization specifies initial neuronal polarization","volume":"180","author":"H Witte","year":"2008","journal-title":"J Cell Biol"},{"issue":"2","key":"pgen.1012106.ref004","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0092-8674(03)00522-1","article-title":"Kinesin superfamily protein 2A (KIF2A) functions in suppression of collateral branch extension","volume":"114","author":"N Homma","year":"2003","journal-title":"Cell"},{"key":"pgen.1012106.ref005","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.30935","article-title":"KIF2A regulates the development of dentate granule cells and postnatal hippocampal wiring","volume":"7","author":"N Homma","year":"2018","journal-title":"Elife"},{"issue":"3","key":"pgen.1012106.ref006","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.neuron.2017.10.021","article-title":"Establishing neuronal polarity with environmental and intrinsic mechanisms","volume":"96","author":"S Yogev","year":"2017","journal-title":"Neuron"},{"key":"pgen.1012106.ref007","doi-asserted-by":"crossref","unstructured":"Desai A, Mitchison TJ. 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The role of the autosomal recessive primary microcephaly protein ASPM: a protein involved in mitosis. 2021."},{"issue":"13","key":"pgen.1012106.ref081","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.4161\/cc.6.13.4356","article-title":"ASPM and citron kinase co-localize to the midbody ring during cytokinesis","volume":"6","author":"M Paramasivam","year":"2007","journal-title":"Cell Cycle"},{"key":"pgen.1012106.ref082","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: protein-protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"D Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res"},{"issue":"22","key":"pgen.1012106.ref083","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1016\/S0960-9822(02)01301-5","article-title":"Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans","volume":"12","author":"F Piano","year":"2002","journal-title":"Curr Biol"},{"issue":"8","key":"pgen.1012106.ref084","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1091\/mbc.e13-11-0687","article-title":"Caenorhabditis elegans oocyte meiotic spindle pole assembly requires microtubule severing and the calponin homology domain protein ASPM-1","volume":"25","author":"AA Connolly","year":"2014","journal-title":"Mol Biol Cell"},{"issue":"5","key":"pgen.1012106.ref085","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/ncb3511","article-title":"Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex","volume":"19","author":"K Jiang","year":"2017","journal-title":"Nat Cell Biol"},{"issue":"11","key":"pgen.1012106.ref086","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.227850","article-title":"Microtubule minus-end regulation at a glance","volume":"132","author":"A Akhmanova","year":"2019","journal-title":"J Cell Sci"},{"issue":"4","key":"pgen.1012106.ref087","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1523\/JNEUROSCI.2341-13.2014","article-title":"p140Cap regulates memory and synaptic plasticity through Src-mediated and citron-N-mediated actin reorganization","volume":"34","author":"D Repetto","year":"2014","journal-title":"J Neurosci"},{"issue":"17","key":"pgen.1012106.ref088","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1091\/mbc.E19-04-0194","article-title":"Rho-dependent control of the Citron kinase, Sticky, drives midbody ring maturation","volume":"30","author":"N El-Amine","year":"2019","journal-title":"Mol Biol Cell"},{"issue":"20","key":"pgen.1012106.ref089","doi-asserted-by":"crossref","first-page":"3768","DOI":"10.1091\/mbc.e10-12-0952","article-title":"Citron kinase controls abscission through RhoA and anillin","volume":"22","author":"M Gai","year":"2011","journal-title":"Mol Biol Cell"},{"issue":"4","key":"pgen.1012106.ref090","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1083\/jcb.201105136","article-title":"Sticky\/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis","volume":"195","author":"ZI Bassi","year":"2011","journal-title":"J Cell Biol"},{"key":"pgen.1012106.ref091","doi-asserted-by":"crossref","first-page":"4819","DOI":"10.1242\/jcs.00163","article-title":"Essential role of citron kinase in cytokinesis of spermatogenic precursors","volume":"115","author":"FD Cunto","year":"2002","journal-title":"J Cell Sci"},{"issue":"13","key":"pgen.1012106.ref092","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.260520","article-title":"Delayed abscission in animal cells - from development to defects","volume":"136","author":"S Kodba","year":"2023","journal-title":"J Cell Sci"},{"issue":"3","key":"pgen.1012106.ref093","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1083\/jcb.200511061","article-title":"KIF14 and citron kinase act together to promote efficient cytokinesis","volume":"172","author":"U Gruneberg","year":"2006","journal-title":"J Cell Biol"},{"issue":"40","key":"pgen.1012106.ref094","article-title":"Mechanistic insights into central spindle assembly mediated by the centralspindlin complex","volume":"118","author":"H Pan","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pgen.1012106.ref095","doi-asserted-by":"crossref","unstructured":"Sarkisian MR, Li W, Cunto FD, D\u2019Mello SR, Loturco JJ. Citron-Kinase, a protein essential to cytokinesis in neuronal progenitors, is deleted in the flathead mutant rat. 2002.","DOI":"10.1523\/JNEUROSCI.22-08-j0001.2002"},{"issue":"3","key":"pgen.1012106.ref096","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/ncb1834","article-title":"NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5\/GPR\/Galpha","volume":"11","author":"M van der Voet","year":"2009","journal-title":"Nat Cell Biol"},{"key":"pgen.1012106.ref097","article-title":"The multiple mitotic roles of the ASPM orthologous proteins: insight into the etiology of ASPM-dependent microcephaly","author":"AV Razuvaeva","year":"2023","journal-title":"Cells"},{"issue":"12","key":"pgen.1012106.ref098","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1212\/WNL.0b013e3181b8799a","article-title":"Expanding the clinical and neuroradiologic phenotype of primary microcephaly due to ASPM 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Biol"},{"issue":"2","key":"pgen.1012106.ref102","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.202311147","article-title":"The kinetochore protein KNL-1 regulates the actin cytoskeleton to control dendrite branching","volume":"224","author":"H Alves Domingos","year":"2025","journal-title":"J Cell Biol"},{"issue":"1","key":"pgen.1012106.ref103","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","article-title":"The genetics of Caenorhabditis elegans","volume":"77","author":"S Brenner","year":"1974","journal-title":"Genetics"},{"key":"pgen.1012106.ref104","first-page":"451","article-title":"Chapter 19 DNA transformation.","volume-title":"Methods in cell biology. 48","author":"C Mello","year":"1995"},{"issue":"3","key":"pgen.1012106.ref105","doi-asserted-by":"crossref","DOI":"10.4161\/worm.25081","article-title":"Deep sequencing strategies for mapping and identifying mutations from genetic screens","volume":"2","author":"S Zuryn","year":"2013","journal-title":"Worm"},{"issue":"1","key":"pgen.1012106.ref106","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1534\/genetics.110.119230","article-title":"A strategy for direct mapping and identification of mutations by whole-genome sequencing","volume":"186","author":"S Zuryn","year":"2010","journal-title":"Genetics"},{"issue":"3","key":"pgen.1012106.ref107","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1002\/bmb.2005.494033032452","article-title":"Isolation of Caenorhabditis elegans genomic DNA and detection of deletions in the unc-93 gene using PCR","volume":"33","author":"JL Lissemore","year":"2005","journal-title":"Biochem Mol Biol Educ"},{"key":"pgen.1012106.ref108","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkac247","article-title":"The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2022 update","volume":"50","author":"Galaxy Community","year":"2022","journal-title":"Nucleic Acids 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Monzon","year":"2022","journal-title":"Bioinform Adv"},{"issue":"6","key":"pgen.1012106.ref121","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1093\/nar\/gkl091","article-title":"M-Coffee: combining multiple sequence alignment methods with T-Coffee","volume":"34","author":"IM Wallace","year":"2006","journal-title":"Nucleic Acids Res"},{"key":"pgen.1012106.ref122","doi-asserted-by":"crossref","DOI":"10.1002\/0471142727.mb2603s109","article-title":"RNA Interference in Caenorhabditis elegans","volume":"109","author":"D Conte","year":"2015","journal-title":"Curr Protoc Mol Biol"},{"issue":"1","key":"pgen.1012106.ref123","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1083\/jcb.147.1.135","article-title":"Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo","volume":"147","author":"P G\u00f6nczy","year":"1999","journal-title":"J Cell 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