{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T18:13:23Z","timestamp":1780596803255,"version":"3.54.1"},"update-to":[{"DOI":"10.1371\/journal.pgen.1012169","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000}}],"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K09330, JP21K06280"],"award-info":[{"award-number":["JP24K09330, JP21K06280"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    DNA double-strand breaks (DSBs) threaten genome stability and cell survival but can be faithfully repaired through homologous recombination (HR). RecN, a bacterial protein closely related to the structural maintenance of chromosomes family, cooperates with RecA in HR-dependent DSB repair, yet the molecular basis and physiological relevance of their interaction remain unclear. Here, we investigated the functional interplay between RecA and RecN during DSB repair by heterologously expressing\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    RecA (paRecA) and RecN (paRecN) in\n                    <jats:italic>Escherichia coli.<\/jats:italic>\n                    We found that in\n                    <jats:italic>E. coli<\/jats:italic>\n                    \u2206\n                    <jats:italic>recA<\/jats:italic>\n                    \u2206\n                    <jats:italic>recN<\/jats:italic>\n                    cells, co-expression of paRecA and paRecN fully restored MMC resistance, whereas co-expression of\n                    <jats:italic>E. coli<\/jats:italic>\n                    RecA (ecRecA) with paRecN conferred partial resistance to mitomycin C (MMC), demonstrating species-specific compatibility. Expression analysis revealed that paRecN was poorly expressed in\n                    <jats:italic>E. coli<\/jats:italic>\n                    , but codon optimization significantly enhanced its abundance and repair activity. We further identified gain-of-function paRecN mutants (I73T and R453H) that restored repair without increased expression. These mutants displayed species-specific adaptation, which improved compatibility with ecRecA but reduced functionality with paRecA. Fluorescence microscopy revealed that MMC-induced nucleoid localization was increased in paRecN\n                    <jats:sup>I73T<\/jats:sup>\n                    and paRecN\n                    <jats:sup>R453H<\/jats:sup>\n                    compared with paRecN. Collectively, these findings demonstrate that coevolution optimizes the RecA\u2013RecN interface to ensure efficient DSB repair.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1012169","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T17:39:52Z","timestamp":1779989992000},"page":"e1012169","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Species-specific coevolution of RecA\u2013RecN interfaces governs DNA double-strand break repair in Escherichia coli"],"prefix":"10.1371","volume":"22","author":[{"given":"Mizuki","family":"Inoue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genki","family":"Akanuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masafumi","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9752-6599","authenticated-orcid":true,"given":"Takashi","family":"Hishida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"issue":"6","key":"pgen.1012169.ref001","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1016\/S1097-2765(01)00419-1","article-title":"Recombination at double-strand breaks and DNA ends: conserved mechanisms from phage to humans","volume":"8","author":"GA 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