{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T04:51:08Z","timestamp":1784868668994,"version":"3.55.0"},"update-to":[{"DOI":"10.1371\/journal.pgen.1012173","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000}}],"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/S009256\/1, BB\/G009325\/1, BBS\/E\/JI\/230002C, BBS\/E\/J\/000PR9773"],"award-info":[{"award-number":["BB\/S009256\/1, BB\/G009325\/1, BBS\/E\/JI\/230002C, BBS\/E\/J\/000PR9773"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Norwich Research Park Biosciences Doctoral Training Partnership","award":["BB\/M011216\/1"],"award-info":[{"award-number":["BB\/M011216\/1"]}]},{"name":"European Research Council Advanced Grant","award":["101055327"],"award-info":[{"award-number":["101055327"]}]}],"content-domain":{"domain":["www.plosgenetics.org"],"crossmark-restriction":false},"short-container-title":["PLoS Genet"],"abstract":"<jats:p>\n                    Identification of the genomic regions that contribute to reproductive isolation and how they interact is a major goal of evolutionary genetics. Much effort has focused on locating candidate genes and potential barrier loci by scanning genomes for regions of excess differentiation (\n                    <jats:italic>F<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>ST<\/jats:italic>\n                    <\/jats:sub>\n                    ). An alternative, and perhaps more robust approach, is to scan for genomic regions exhibiting steep clines in allele frequency across a hybrid zone. We develop a computationally efficient method for approximating cline parameters for large number of loci, and apply it to genomic data from across a hybrid zone between flower colour varieties of\n                    <jats:italic>Antirrhinum majus<\/jats:italic>\n                    (\n                    <jats:italic>A. m. m<\/jats:italic>\n                    var.\n                    <jats:italic>pseudomajus<\/jats:italic>\n                    and\n                    <jats:italic>A. m. m<\/jats:italic>\n                    var.\n                    <jats:italic>striatum<\/jats:italic>\n                    ). Most steep clines are clustered in seven genomic regions, only four of which were present from\n                    <jats:italic>F<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>ST<\/jats:italic>\n                    <\/jats:sub>\n                    scans between all pair-wise comparisons. Six of these regions carry previously identified loci that influence flower colour in the hybrid zone. The seventh region harbours a novel locus\n                    <jats:italic>, RUBIA<\/jats:italic>\n                    , modifying magenta intensity. Clines at\n                    <jats:italic>RUBIA<\/jats:italic>\n                    approached fixation on the magenta side of the hybrid zone, whilst remaining polymorphic on the yellow side. This polymorphism on the yellow side may reflect a smaller phenotypic effect of\n                    <jats:italic>RUBIA<\/jats:italic>\n                    in yellow compared to magenta genetic backgrounds. Our findings illustrate how whole-genome cline scans in hybrid zones can robustly detect genomic regions contributing to phenotypic differences and highlight how different reproductive barrier loci interact across the genome.\n                  <\/jats:p>","DOI":"10.1371\/journal.pgen.1012173","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T17:42:16Z","timestamp":1783964536000},"page":"e1012173","update-policy":"https:\/\/doi.org\/10.1371\/journal.pgen.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genome-wide cline analysis identifies new locus contributing to a barrier to gene flow across an Antirrhinum hybrid zone"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4014-8478","authenticated-orcid":true,"given":"David 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